LOCUS       sequence-36482-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Arnold CP, Tan R, Zhou B, Yue SB, Schaffert S, Biggs JR, Doyonnas R,
            Lo MC, Perry JM, Renault VM, Sacco A, Somervaille T, Viatour P, 
            Brunet A, Cleary ML, Li L, Sage J, Zhang DE, Blau HM, Chen C, Chen 
            CZ
  TITLE     MicroRNA programs in normal and aberrant stem and progenitor cells.
  JOURNAL   Genome Res. 2011 May . 21(5):798-810.
  PUBMED    21451113
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2011 May . 21(5):798-810."
COMMENT     Sequence Label: MDH1-miR-196a-2-PGK-GFP-Blast
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    108..569
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(164..181)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     391..410
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ggcanctggc aggngagctg tacaagtaat gaggatcacc gggcaattga agatctagtc
       61 gaccaattcc ggttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
      121 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
      181 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
      241 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
      301 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
      361 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
      421 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
      481 tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg
      541 tggttttcct ttgaaaaaca cgatgataat accatgaaaa catttaacat ttctcaacag
      601 gatctagaat tagtagaagt agcgacagag aagattacaa tgctttatga ggataataaa
      661 catcatgtgg gagcggcaat tcgtacgaaa acaggagaaa tcatttcggc agtacatatt
      721 gaagcgtata taggacgagt aactgtttgt gcagaagcca ttgcgattgg tagtgcagtt
      781 tcgaatggac aaaaggattt tgacacgatt gtagctgtta gacaccctta ttctgacgaa
      841 gtagatagaa gtattcgagt ggtaagtcct tgtggtatgt gtagggagtt gatttcagac
      901 tatgcaccag attgttttgt gttaatagaa atgaatggca agttagtcaa atnnatgaga
      961 tctcccaaac na
//
