LOCUS       sequence-364838-        1647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CapChR1-mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Fernandez Lahore RG, Pampaloni NP, Peter E, Heim MM, Tillert L, 
            Vierock J, Oppermann J, Walther J, Schmitz D, Owald D, Plested AJR, 
            Rost BR, Hegemann P
  TITLE     Calcium-permeable channelrhodopsins for the photocontrol of calcium 
            signalling.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4.
  PUBMED    36543773
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7844. doi"; pages: " 
            10.1038/s41467-022-35373-4"
COMMENT     Sequence Label: CapChR1_pmCherry-N1
FEATURES             Location/Qualifiers
     source          1..1647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..927
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFDILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKECWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             940..1647
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1082..1100)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1350..1369
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggactatg gcggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt tactaatcct
       61 gtggtggtga acgggtccgt cctggtccct gaggatcaat gttactgtgc cggatggatt
      121 gaatctcgcg gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg gcttgcagca
      181 ggattcgaca ttttgctgct gatgttctat gcctaccaaa cctggaaatc tacatgcggc
      241 tgggaggaga tctatgtgtg cgccattgaa atggttaagg tgattctcga gttctttttt
      301 gagtttaaga atccctctat gctctacctt gccacaggac accgggtgca gtggctgcgc
      361 tatgcagagt ggctgctcac ttgtcctgtc atctgtatcc acctgagcaa cctcaccggc
      421 ctgagcaacg actacagcag gagaaccatg ggactccttg tctcagacat cgggtgtatc
      481 gtgtgggggg ctaccagcgc catggcaacc ggctatgtta aagtcatctt cttttgtctt
      541 ggattgtgct atggcgcgaa cacatttttt cacgccgcca aagcatatat cgagggttat
      601 catactgtgc caaagggtcg gtgccgccag gtcgtgaccg gcatggcatg gctgtttttc
      661 gtgagctggg gtatgttccc aattctcttc attttggggc ccgaaggttt tggcgtcctg
      721 agcgtctatg gctccaccgt aggtcacacg attattgatc tgatgagtaa agagtgttgg
      781 gggttgttgg gacactacct gcgcgtcctg atccacgagc acatattgat tcacggagat
      841 atccgcaaaa ccaccaaact gaacatcggc ggaacggaga tcgaggtcga gactctcgtc
      901 gaagacgaag ccgaggccgg agccgtgcca gcggccgccg tgagcaaggg cgaggaggat
      961 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     1021 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1081 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     1141 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     1201 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     1261 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     1321 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     1381 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     1441 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     1501 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     1561 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     1621 ggcggcatgg acgagctgta caagtaa
//
