LOCUS       sequence-364841-        1578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CapChR2-mScarlet in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Fernandez Lahore RG, Pampaloni NP, Peter E, Heim MM, Tillert L, 
            Vierock J, Oppermann J, Walther J, Schmitz D, Owald D, Plested AJR, 
            Rost BR, Hegemann P
  TITLE     Calcium-permeable channelrhodopsins for the photocontrol of calcium 
            signalling.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4.
  PUBMED    36543773
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7844. doi"; pages: " 
            10.1038/s41467-022-35373-4"
COMMENT     Sequence Label: CapChR2_pmScarlet-N1
FEATURES             Location/Qualifiers
     source          1..1578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..864
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFDIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVICIHLSNLTGLKDDYSKRTMRLLVSDVGCIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSKECWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     CDS             883..1575
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(1013..1031)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1281..1300
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgctgggaa acggcagcgc cattgtgcct atcgaccagt gcttttgcct ggcttggacc
       61 gacagcctgg gaagcgatac agagcagctg gtggccaaca tcctccagtg gttcgccttc
      121 ggcttcgaca tcctgatcct gatgttctac gcctaccaga cttggagagc cacttgcggt
      181 tgggaggagg tctacgtctg ttgcgtcgag ctgaccaagg tcatcatcga gttcttccac
      241 gagttcgacg accccagcat gctgtacctg gctaacggac accgagtcca gtggctgaga
      301 tacgcagagt ggctgctgac ttgtcccgtc atctgcatcc acctgagcaa cctgaccggc
      361 ctgaaggacg actacagcaa gcggaccatg aggctgctgg tgtcagacgt gggatgcatc
      421 gtgtggggag ctacaagcgc catgagcaca ggctacgtca aggtcatctt cttcgtgctg
      481 ggttgcatct acggcgccaa caccttcttc cacgccgcca aggtgtatat cgagagctac
      541 cacgtggtgc caaagggcag acctagaacc gtcgtgcgga tcatggcttg gctgttcttc
      601 ctgtcttggg gcatgttccc cgtgctgttc gtcgtgggac cagaaggatt cgacgccatc
      661 agcgtgtacg gctctaccat tggccacacc atcatcgacc tcatgagcaa ggagtgttgg
      721 ggcctgctgg gacactatct gagagtgctg atccaccagc acatcatcat ctacggcgac
      781 atccggaaga agaccaagat caacgtggcc ggcgaggaga tggaagtgga gaccatggtg
      841 gaccaggagg acgaggagac agtggcggca ccggtagtag cagtgagcaa gggcgaggca
      901 gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag
      961 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     1021 aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg
     1081 tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta taagcagtcc
     1141 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc
     1201 gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc
     1261 accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc
     1321 accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat ggccctgcgc
     1381 ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc caagaagccc
     1441 gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc ccacaacgag
     1501 gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac cggcggcatg
     1561 gacgagctgt acaagtaa
//
