LOCUS       sequence-364850-        1632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CapChR1-mScarlet in mammalian neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Fernandez Lahore RG, Pampaloni NP, Peter E, Heim MM, Tillert L, 
            Vierock J, Oppermann J, Walther J, Schmitz D, Owald D, Plested AJR, 
            Rost BR, Hegemann P
  TITLE     Calcium-permeable channelrhodopsins for the photocontrol of calcium 
            signalling.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4.
  PUBMED    36543773
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7844. doi"; pages: " 
            10.1038/s41467-022-35373-4"
COMMENT     Sequence Label: pAAV-Syn_CapChR1_mScarlet
FEATURES             Location/Qualifiers
     source          1..1632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..927
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFDILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKECWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             940..1632
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(1070..1088)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1338..1357
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggactatg gcggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt tactaatcct
       61 gtggtggtga acgggtccgt cctggtccct gaggatcaat gttactgtgc cggatggatt
      121 gaatctcgcg gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg gcttgcagca
      181 ggattcgaca ttttgctgct gatgttctat gcctaccaaa cctggaaatc tacatgcggc
      241 tgggaggaga tctatgtgtg cgccattgaa atggttaagg tgattctcga gttctttttt
      301 gagtttaaga atccctctat gctctacctt gccacaggac accgggtgca gtggctgcgc
      361 tatgcagagt ggctgctcac ttgtcctgtc atctgtatcc acctgagcaa cctcaccggc
      421 ctgagcaacg actacagcag gagaaccatg ggactccttg tctcagacat cgggtgtatc
      481 gtgtgggggg ctaccagcgc catggcaacc ggctatgtta aagtcatctt cttttgtctt
      541 ggattgtgct atggcgcgaa cacatttttt cacgccgcca aagcatatat cgagggttat
      601 catactgtgc caaagggtcg gtgccgccag gtcgtgaccg gcatggcatg gctgtttttc
      661 gtgagctggg gtatgttccc aattctcttc attttggggc ccgaaggttt tggcgtcctg
      721 agcgtctatg gctccaccgt aggtcacacg attattgatc tgatgagtaa agagtgttgg
      781 gggttgttgg gacactacct gcgcgtcctg atccacgagc acatattgat tcacggagat
      841 atccgcaaaa ccaccaaact gaacatcggc ggaacggaga tcgaggtcga gactctcgtc
      901 gaagacgaag ccgaggccgg agccgtgcca gcggccgccg tgagcaaggg cgaggcagtg
      961 atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg ccacgagttc
     1021 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1081 gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca gttcatgtac
     1141 ggctccaggg ccttcaccaa gcaccccgcc gacatccccg actactataa gcagtccttc
     1201 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc cgtgaccgtg
     1261 acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct ccgcggcacc
     1321 aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga agcgtccacc
     1381 gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc cctgcgcctg
     1441 aaggacggcg gccgctacct ggcggacttc aagaccacct acaaggccaa gaagcccgtg
     1501 cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca caacgaggac
     1561 tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg cggcatggac
     1621 gagctgtaca ag
//
