LOCUS       sequence-364876-        7709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) 
            in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y
  TITLE     Establishment of strigolactone-producing bacterium-yeast consortium.
  JOURNAL   Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. 
            Epub 2021 Sep 17.
  PUBMED    34533983
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-09-17"; volume: "7(38):eabh4048. doi"; pages: " 
            10.1126/sciadv.abh4048. Epub 2021 Sep 17"
COMMENT     Sequence Label: p413TEF1-ATR1(pYL573)
FEATURES             Location/Qualifiers
     source          1..7709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        623..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2622..2643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2755..2775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2806..2830
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2836..3234
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      5336..5581
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5351..5369)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    complement(5582..5606)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5642..5661)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5643..5661)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5671..5688)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5671..5687)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5680..5702)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5828..6283
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5915..5934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6125..6146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6543..7205)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(7206..7393)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(7540..7559)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7659..7681
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgccwattwk krtwksykmr yrymwtkmym mkaawrgtky
       61 cwymkgryss wtykcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 attwaaatwm karkyryktk tawrkyacrk gmwrrmraks msycwrrkrr mmcwttwykr
      601 ksawrwswky rmssaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctcccgcggc ccatcacaag tttgtacaaa
     2821 aaagcaggct atatgcatag cttcaaaatg tctctactcc ttttttactc ttccagattt
     2881 tctcggactc cgcgcatcgc cgtaccactt caaaacaccc aagcacagca tactaaattt
     2941 cccctctttc ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa
     3001 aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt
     3061 tctttttctt gaaaattttt tttttgattt ttttctcttt cgatgacctc ccattgatat
     3121 ttaagttaat aaacggtctt caatttctca agtttcagtt tcatttttct tgttctatta
     3181 caactttttt tacttcttgc tcattagaaa gaaagcatag caatctaatc taagttttcc
     3241 taggtataca atgacttctg ccttgtatgc ctctgatttg ttcaagcaat tgaagtccat
     3301 tatgggcacc gattctttgt ctgatgatgt tgttttggtt atcgctacta cctctttggc
     3361 tttggttgct ggttttgttg ttctgttgtg gaaaaggact accgctgata gatctggtga
     3421 attgaaacca ttgatgatcc ccaaatcttt gatggccaaa gatgaagatg atgacttgga
     3481 cttaggttct ggtaagacta gagtttccat tttcttcggt actcaaactg gtactgctga
     3541 aggttttgct aaagctttgt ccgaagaaat caaggccaga tacgaaaaag ctgccgttaa
     3601 ggttattgat ttggatgatt atgctgccga tgacgaccaa tacgaagaaa agttgaagaa
     3661 agaaaccttg gccttcttct gtgttgctac ttatggtgat ggtgaaccta ctgataatgc
     3721 tgctagattt tacaagtggt tcaccgaaga gaacgaaaga gatatcaagt tgcaacaatt
     3781 ggcctacggt gtttttgctt tgggtaatag acaatacgag cacttcaaca agatcggtat
     3841 cgttttggat gaagagttgt gtaaaaaggg tgccaagaga ttgattgaag ttggtttggg
     3901 tgatgatgac cagtctatcg aagatgattt taacgcctgg aaagaatcct tgtggtctga
     3961 attggataag ttgttgaagg acgaagatga caaatctgtt gctacaccat acactgctgt
     4021 tattccagag tatagagttg ttactcacga tccaagattc acgactcaaa agtctatgga
     4081 atctaacgtt gctaacggta acaccaccat cgatattcat catccatgta gagttgatgt
     4141 cgccgtccaa aaagaattgc atactcatga atccgacaga tcctgcattc atttggaatt
     4201 cgatatttcc agaaccggta ttacttacga aaccggtgat catgttggtg tttacgctga
     4261 aaatcacgtt gaaatcgttg aagaagccgg taagttgtta ggtcattcat tggatttggt
     4321 gttctccatt catgccgaca aagaagatgg ttctcctttg gaatctgctg ttccaccacc
     4381 atttccaggt ccatgtactt taggtactgg tttggctaga tatgctgact tgttgaatcc
     4441 accaagaaag tctgctttag ttgctttggc tgcttatgct actgaaccat ctgaagccga
     4501 aaaattgaaa catttgactt ccccagatgg taaggacgaa tattctcaat ggatagttgc
     4561 ctctcagagg tctttgttgg aagttatggc tgcttttcca tctgctaaac caccattggg
     4621 tgtttttttt gctgctattg ctccaagatt gcaacctagg tattactcca tttcttcatc
     4681 accaagattg gccccatcta gagttcatgt tacatctgct ttggtttatg gtccaactcc
     4741 aactggtaga attcataagg gtgtttgttc tacctggatg aagaacgctg ttccagctga
     4801 aaaatctcat gaatgttctg gtgccccaat tttcattaga gcttctaatt tcaagctgcc
     4861 aagcaatcca tctactccaa tagttatggt tggtccaggt acaggtttag ctccttttag
     4921 aggtttccta caagaaagga tggccttgaa agaggatggc gaagaattgg gttcttcctt
     4981 gttgtttttt ggttgcagaa acagacagat ggatttcatc tatgaggacg agttgaacaa
     5041 cttcgttgat caaggtgtta tctccgaatt gattatggcc ttttctagag aaggtgccca
     5101 gaaagaatat gtccaacata agatgatgga aaaagccgct caagtttggg acctaatcaa
     5161 agaagaagga tacttgtacg tttgcggtga tgctaaaggt atggctagag atgttcatag
     5221 aacattgcat accatcgtcc aagaacaaga aggtgtttca tcttctgaag ctgaagctat
     5281 cgttaagaag ttgcaaactg aaggtagata cttgagagat gtctggtgac tcgagtcatg
     5341 tattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg
     5401 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
     5461 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
     5521 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
     5581 gacccagctt tcttgtacaa agtggtgatg gggcggccgc gtttaaacgg tacccaattc
     5641 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     5701 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5761 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5821 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5881 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5941 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6001 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6061 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6121 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6181 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6241 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     6301 tccttacgca tctgtgcggt atttcacacc gcatagatcc gtcgagttca agagaaaaaa
     6361 aaagaaaaag caaaaagaaa aaaggaaagc gcgcctcgtt cagaatgaca cgtatagaat
     6421 gatgcattac cttgtcatct tcagtatcat actgttcgta tacatactta ctgacattca
     6481 taggtataca tatatacaca tgtatatata tcgtatgctg cagctttaaa taatcggtgt
     6541 cactacataa gaacaccttt ggtggaggga acatcgttgg taccattggg cgaggtggct
     6601 tctcttatgg caaccgcaag agccttgaac gcactctcac tacggtgatg atcattcttg
     6661 cctcgcagac aatcaacgtg gagggtaatt ctgctagcct ctgcaaagct ttcaagaaaa
     6721 tgcgggatca tctcgcaaga gagatctcct actttctccc tttgcaaacc aagttcgaca
     6781 actgcgtacg gcctgttcga aagatctacc accgctctgg aaagtgcctc atccaaaggc
     6841 gcaaatcctg atccaaacct ttttactcca cgcacggccc ctagggcctc tttaaaagct
     6901 tgaccgagag caatcccgca gtcttcagtg gtgtgatggt cgtctatgtg taagtcacca
     6961 atgcactcaa cgattagcga ccagccggaa tgcttggcca gagcatgtat catatggtcc
     7021 agaaacccta tacctgtgtg gacgttaatc acttgcgatt gtgtggcctg ttctgctact
     7081 gcttctgcct ctttttctgg gaagatcgag tgctctatcg ctaggggacc accctttaaa
     7141 gagatcgcaa tctgaatctt ggtttcattt gtaatacgct ttactagggc tttctgctct
     7201 gtcatctttg ccttcgttta tcttgcctgc tcatttttta gtatattctt cgaagaaatc
     7261 acattacttt atataatgta taattcatta tgtgataatg ccaatcgcta agaaaaaaaa
     7321 agagtcatcc gctaggtgga aaaaaaaaaa tgaaaatcat taccgaggca taaaaaaata
     7381 tagagtgtac tagaggaggc caagagtaat agaaaaagaa aattgcggga aaggactgtg
     7441 ttatgacttc cctgactaat gccgtgttca aacgatacct ggcagtgact cctagcgctc
     7501 accaagctct taaaacggga atttatggtg cactctcagt acaatctgct ctgatgccgc
     7561 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7621 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     7681 gttttcaccg tcatcaccga aacgcgcga
//
