LOCUS       sequence-364880-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Mukherjee S, Manna P, Hung ST, Vietmeyer F, Friis P, Palmer AE, 
            Jimenez R
  TITLE     Directed Evolution of a Bright Variant of mCherry: Suppression of 
            Nonradiative Decay by Fluorescence Lifetime Selections.
  JOURNAL   J Phys Chem B. 2022 Jun 16. doi: 10.1021/acs.jpcb.2c01956.
  PUBMED    35709514
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Phys Chem
            B. 2022 Jun 16. doi: 10.1021/acs.jpcb.2c01956."
COMMENT     Sequence Label: pBad-mCherry-XL (R125K)
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             77..99
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             119..136
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             140..172
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(176..184,185..199)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(357..375)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     625..644
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatgcgggtc tacctgacgc tttttatcgc actctctact gtttctccat acccgttttt
       61 ttgggctaga aataattttg tttaacttta agaaggagat atacatatgc ggggttctca
      121 tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct
      181 gtacgacgat gacgataagg atcccaaaaa aatggtgagc aagggcgagg aggataacat
      241 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      301 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      361 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      421 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      481 gtcattcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      541 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgaa
      601 gggcaccaac tttccctccg acggccccgt aatgcagaag aagaccatgg gcagtgaggc
      661 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgaggtta agtataggct
      721 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
      781 gcccgtgcag ctgcccggcg cctacaacgt caaccgtaag ttggacatca cctcccacaa
      841 cgaggactac accatcgtgg aacagtacga acgcgccgag ggcgcactcc accggcggca
      901 tggacgagct gtacagtaga tcgagcttgc tgttttgcga tgaagaagat ttcagctgat
      961 acagataatt cgacgcagag ccgtctgata aacgaatttg tctgg
//
