LOCUS       sequence-36489-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Arnold CP, Tan R, Zhou B, Yue SB, Schaffert S, Biggs JR, Doyonnas R,
            Lo MC, Perry JM, Renault VM, Sacco A, Somervaille T, Viatour P, 
            Brunet A, Cleary ML, Li L, Sage J, Zhang DE, Blau HM, Chen C, Chen 
            CZ
  TITLE     MicroRNA programs in normal and aberrant stem and progenitor cells.
  JOURNAL   Genome Res. 2011 May . 21(5):798-810.
  PUBMED    21451113
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2011 May . 21(5):798-810."
COMMENT     Sequence Label: MDH1-miR-324SM-PGK-GFP-Blast
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    102..563
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(158..175)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     385..404
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ctggcnggng agctgtacag taatgaggat caccgggcaa ttgaagatct agtcgaccaa
       61 ttccggttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
      121 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
      181 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
      241 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
      301 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
      361 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
      421 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
      481 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
      541 tcctttgaaa aacacgatga taataccatg aaaacattta acatttctca acaggatcta
      601 gaattagtag aagtagcgac agagaagatt acaatgcttt atgaggataa taaacatcat
      661 gtgggagcgg caattcgtac gaaaacagga gaaatcattt cggcagtaca tattgaagcg
      721 tatataggac gagtaactgt ttgtgcagaa gccattgcga ttggtagtgc agtttcgaat
      781 ggacaaaagg attttgacac gattgtagct gttagacacc cttattctga cgaagtagat
      841 agaagtattc gagtggtaag tccttgtggt atgtgtaggg agttgatttc agactatgca
      901 ccagattgtt ttgtgttaat agaaatgaat ggcagtanca atcatgaatc tctcanccat
      961 a
//
