LOCUS       sequence-364896-        6190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 
            from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from 
            Arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y
  TITLE     Establishment of strigolactone-producing bacterium-yeast consortium.
  JOURNAL   Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. 
            Epub 2021 Sep 17.
  PUBMED    34533983
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-09-17"; volume: "7(38):eabh4048. doi"; pages: " 
            10.1126/sciadv.abh4048. Epub 2021 Sep 17"
COMMENT     Sequence Label: pCDFDuet-trAtCCD7-OsD27(pYL726 )
FEATURES             Location/Qualifiers
     source          1..6190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(210..1292)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTXLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1254..1273
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1293..1370)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        1416..1434
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1435..1459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        3350..3368
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3369..3393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4288..4306)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4302..4349
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4520..5311)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(5312..5403)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5451..6190)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag
      181 tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      301 gccagggkgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc
      361 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
      421 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc
      481 actaccgaga tgtccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
      541 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt
      601 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc
      661 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca
      721 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc
      781 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca
      841 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc
      901 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
      961 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
     1021 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc
     1081 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg
     1141 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca
     1201 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac
     1261 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc
     1321 gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg
     1381 acgctctccc ttatgcgact cctgcattag gaaattaata cgactcacta taggggaatt
     1441 gtgagcggat aacaattccc ctgtagaaat aattttgttt aactttaata aggagatata
     1501 ccatgggcgc tccaccaaga gctgctattt ctatttcaat tccagatacc ggtttgggta
     1561 gaaccggtac tattttggat gaatctactt cctctgcctt cagagattac caatctttgt
     1621 tcgtgtctca gagatccgaa actattgaac cagttgttat caagccaatc gaaggttcta
     1681 ttccagttaa ttttccatct ggcacttact atttggctgg tccaggtttg tttactgatg
     1741 atcatggttc tactgttcac ccattggatg gtcatggtta tttgagagct ttccatatcg
     1801 atggtaacaa gagaaaggct actttcactg ctaagtacgt taagaccgaa gccaaaaaag
     1861 aagaacacga tccagttact gatacttgga gattcactca tagaggtcca ttctctgttt
     1921 tgaaaggtgg taagagattc ggtaacacca aggttatgaa gaacgttgct aacacttccg
     1981 ttttgaaatg ggctggtaga ttattgtgtt tgtgggaagg tggtgaacca tacgaaattg
     2041 aatctggttc tttggatacc gtcggtagat tcaatgttga aaacaacggt tgcgaatctt
     2101 gcgacgatga tgattcttct gatagagatt tgtccggtca tgatatttgg gatactgctg
     2161 ctgatttgtt gaagccaatt ctacaaggtg ttttcaagat gccaccaaag agattcttgt
     2221 cccattacaa agttgacggt agaagaaaga ggttgttgac tgttacttgt aacgccgaag
     2281 atatgttgtt gccaagatct aacttcacct tctgcgaata cgattccgaa ttcaagttga
     2341 ttcagaccaa agagttcaag atcgacgatc acatgatgat ccatgattgg gcttttaccg
     2401 atactcacta cattttgttt gccaacagag tcaagttgaa cccaattggt tctattgctg
     2461 ctatgtgtgg tatgtctcca atggtttctg ctttgtcttt gaacccatct aacgaatctt
     2521 ccccaatcta tattttgcca aggttctccg ataagtactc tagaggtggc agagattgga
     2581 gagttccagt tgaagtttct tctcaattgt ggttgatcca ttctggtaac gcttacgaaa
     2641 ctagagaaga taacggtgac ttgaagattc aaattcaagc ttctgcttgc tcctacagat
     2701 ggtttgattt tcaaaagatg ttcggttacg actggcagtc taacaaattg gatccatctg
     2761 ttatgaactt gaacagaggt gatgacaagt tgttaccaca cttggttaag gtttctatga
     2821 ccttggattc taccggtaac tgtaactctt gtgatgttga acctttgaac ggttggaaca
     2881 agccatctga ttttccagtt attaactcct cttggtccgg caaaaaaaac aagtatatgt
     2941 actctgctgc ctcctctggt actagatctg aattgccaca ttttccattc gatatggttg
     3001 tcaagttcga cttggactct aacttggtta gaacttggtc tactggtgct agaagatttg
     3061 ttggtgaacc tatgttcgtc ccaaagaact ctgttgaaga gggtgaagag gaagatgacg
     3121 gttatatcgt tgttgttgaa tacgccgttt ctgtcgaaag atgctacttg gttattttgg
     3181 acgccaaaaa gatcggtgaa tctgatgctg ttgtttccag attagaagtc ccaagaaatc
     3241 tgactttccc aatgggtttt catggtttgt gggcttcaga ttgagcggcc gcataatgct
     3301 taagtcgaac agaaagtaat cgtattgtac acggccgcat aatcgaaatt aatacgactc
     3361 actatagggg aattgtgagc ggataacaat tccccatctt agtatattag ttaagtataa
     3421 gaaggagata tacatatgga aactaccacc ttggttttgt tgttgccaca tggtggtgct
     3481 ggtggtgtta gaccagctgc tgctgctact gctaaaagat cttatgttat gagaagatgc
     3541 tgctccactg ttagagctgt tatggctaga cctcaagaag ctccagcttc tgctccagct
     3601 aaaaaaactg aaactgctgc tatgatgtct accgttcaaa cagaaactgc agctgcccca
     3661 ccagctactg tttacagaga ttcttggttt gataagttgg ccatcggtta cttgtctagg
     3721 aacttgcaag aagcttcagg tttgaagaac gaaaaggatg gttacgaatc cttgattgat
     3781 gctgctttgg ccatctccag aattttctca ttggataagc aatccgaaat cgttacccaa
     3841 gctttggaaa gagctttgcc atcttacatt ttgaccatga tcaaggttat gatgccacca
     3901 tctagattct ccagagaata ctttgctgct ttcaccacta ttttcttccc atggttggtt
     3961 ggtccatgtg aagttatgga atctgaagtc gaaggcagaa aagaaaagaa cgttgtttac
     4021 atcccaaagt gcaggttctt ggaatctact aattgtgttg gtatgtgcac caacttgtgt
     4081 aagattccat gccaaaagtt catccaggat tctttgggta tgaaggttta catgtctcca
     4141 aacttcgaag atatgtcctg cgaaatgatt ttcggtcaac aaccaccaga agatgatcca
     4201 gctttgaaac aaccatgttt cagaactaag tgcgttgcca aacaaaatca tggtgttaac
     4261 tgctccatct aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     4321 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
     4381 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     4441 aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc
     4501 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
     4561 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
     4621 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     4681 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     4741 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     4801 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     4861 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     4921 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     4981 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     5041 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     5101 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     5161 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     5221 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     5281 tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca
     5341 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5401 aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
     5461 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
     5521 cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
     5581 aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
     5641 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct
     5701 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac
     5761 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt
     5821 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc
     5881 ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc
     5941 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
     6001 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
     6061 agttaccacg gttaagncag ttssssaact gncttaacct tcgatcaaac cacctcccca
     6121 ggtggttttt tcgwwkrcwr rgcmaaagat tacgcgcaga aaaaaaggat ctcaagaaga
     6181 tcctttgatc
//
