LOCUS       sequence-364899-       11739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral delivery of recombinase landing pad .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT,
            Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD
  TITLE     Systematic discovery of recombinases for efficient integration of 
            large DNA sequences into the human genome.
  JOURNAL   Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 
            10.1038/s41587-022-01494-w.
  PUBMED    36217031
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 
            10.1038/s41587-022-01494-w."
COMMENT     Sequence Label: pJT303 Ec04 Lenti  pEF-attB LSR GFP
FEATURES             Location/Qualifiers
     source          1..11739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2682..3860
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2913..3851
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3808..3828
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3971..3980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5636..5689
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5690..5692,5693..6406)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5690..5692,5693..6403)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5732..5753)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5993..6012)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6340..6361
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6431..7019
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6484..6504)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6902..6913)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7022..7038)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7023..7039)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7544..7724
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7750..7767)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7756..7980
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8026..8454
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8113..8132)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8323..8344
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8463..8483)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8468..8797
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8648..8783
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8710..8729
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8845..8892
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8911..9285
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9415..9536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9452..9471)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9506..9525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9585..9601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9585..9601)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9598..9620)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9609..9625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9633..9639,9640..9657,9658..9663))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9678..9699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9816..9833)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9987..10575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10067..10086)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10746..11537,11538..11606))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11369..11388
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11607..11711)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     3001 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3061 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3121 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3181 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3241 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3301 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3361 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3421 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3481 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3541 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3601 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3661 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3721 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3781 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3841 ttccatttca ggtgtcgtga ctacggaata gagataacac gaggagtggt tagaaatggc
     3901 taaagttctg gtgctttatt attccatgta cggacatatt gaaacgatgg cacgcgcagt
     3961 cgctgaggcc gccaccatgg gcaagctgct ggtcacctac atcagatggt ccaccaagga
     4021 acaggactct ggcgacagcc tgcggagaca gaccaacctg atcgatgcct tttacagcaa
     4081 gcacaagaac gactactacc tgctgcccgc tcacagatac gtggacaaag gcaaaagcgg
     4141 ctttcaccaa caacacaagg cccagggcag cgacttcaga cggatgttcg agaacgtgat
     4201 gtctggcgcc atccccgagg gcagcctgat cgtggtggaa aacttcgaca gattcagtag
     4261 agccgacatc gacaccgcca ttgatgacgt gcggcagatc ctccgaaagg gagtgtccat
     4321 cctgacactc ggcgatggcg aactgtacga caagtccgcc ctgaccgacc ctgttaagct
     4381 gatcaagcac atcatcatcg ccgagagagc ccatcaggag agcctggtga agcaaaagag
     4441 aatcgcccaa gtgtggaacc acaagaccca gctggcccgg gagctgaaga aacctatggg
     4501 caagcaggcc cctggctggc tggaactgtc tgaggacggc agccactaca tcgtcgatga
     4561 agataaggcc tctctggtca acatcatcta cgacaagagg ctgagcggga tgagcatgtt
     4621 tgccatctgc aagtggctga acgagcaggg atatcctacc atcaaccaga gaaaggtgcg
     4681 gatttctaag acaaagaagc ccgacggaaa ttggagcgcc ctgtccgtga aacatatcct
     4741 gacaagcaga agcgtgctgg gctacctgcc agctaagatc agcaccgagg atagaaagac
     4801 cgtgctgaga gaagagatcg agggcttcta ccctcagatc gtgacagaca gcaaattcta
     4861 cgccgtgcag cagctgctgg aagagacagg caaaggcaag acgagcagcg gcgagcactg
     4921 gctgtacgtg aatatcctga agggcctgat ccgatgtaaa tgcggcctgg tgatgacccc
     4981 tacaggcatc cggaagcctg tgtaccaggg cacctacaga tgcaacggca acaaggaaag
     5041 cagatgtagc tacggcacag tgtcccggaa actgctggat acacagttgt gcagcagact
     5101 gttcagcaag ctctcccagc tccacgacga agcaaccgac accgccaagc tggacgagct
     5161 gcagcggaga ctgaacaccg tggacagcga gctggaaaag ctgacagaga cactgatcca
     5221 gctgccaaac atcacccaga ttcaggaggc cctgagagtg aagcaggagg aaaaggacga
     5281 gctgatcgtg cagctaagca gagagaaagc cagagtgaaa agcgtgtcta gcctggacct
     5341 gagcggactg gacatggaaa gtgtggaagg cagaaccgag gcccagatca tcatcaagcg
     5401 gctggtgaag gaaatcgtcg tgtccggcaa cgagaagctg gttgatatct acctgcacaa
     5461 cggcaatatg atcagaggct tccccctgga cggaaaagac gaccacaccc tgaccctgga
     5521 agaagctaca gatgagatgc agagcctgga tgatatgctg attttcggcg agcctgtgac
     5581 cagaatctac cctgctggcg acatggaaga ggtggacgcc gctagcggca gtggagaggg
     5641 cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccag tgagcaaggg
     5701 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     5761 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     5821 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     5881 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     5941 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     6001 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     6061 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     6121 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     6181 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     6241 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     6301 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     6361 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagaat tcgatatcaa
     6421 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6481 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6541 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6601 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6661 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6721 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6781 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6841 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6901 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6961 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7021 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     7081 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     7141 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7201 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     7261 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     7321 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     7381 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     7441 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     7501 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     7561 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7621 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7681 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     7741 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7801 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7861 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7921 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7981 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8041 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8101 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8161 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8221 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8281 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8341 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8401 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     8461 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8521 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8641 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8701 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8761 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     8821 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     8881 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     8941 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     9001 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     9061 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     9121 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     9181 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     9241 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     9301 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     9361 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     9421 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9481 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9541 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     9601 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9661 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9721 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9781 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9841 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9901 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9961 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10021 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10081 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10141 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10201 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10261 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10321 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10381 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10441 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10501 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10561 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10621 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10681 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10741 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10801 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10861 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10921 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10981 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11041 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11101 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11161 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11221 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11281 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11341 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11401 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11461 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11521 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11581 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11641 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11701 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
