LOCUS       sequence-36491-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Arnold CP, Tan R, Zhou B, Yue SB, Schaffert S, Biggs JR, Doyonnas R,
            Lo MC, Perry JM, Renault VM, Sacco A, Somervaille T, Viatour P, 
            Brunet A, Cleary ML, Li L, Sage J, Zhang DE, Blau HM, Chen C, Chen 
            CZ
  TITLE     MicroRNA programs in normal and aberrant stem and progenitor cells.
  JOURNAL   Genome Res. 2011 May . 21(5):798-810.
  PUBMED    21451113
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2011 May . 21(5):798-810."
COMMENT     Sequence Label: MDH1-miR-221SM-PGK-GFP-Blast
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    105..566
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(161..178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     388..407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gnantctgga ggagagctgt cagtaatgag gatcaccggg caattgaaga tctagtcgac
       61 caattccggt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
      121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
      181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
      241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
      301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
      361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
      421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
      481 tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg
      541 ttttcctttg aaaaacacga tgataatacc atgaaaacat ttaacatttc tcaacaggat
      601 ctagaattag tagaagtagc gacagagaag attacaatgc tttatgagga taataaacat
      661 catgtgggag cggcaattcg tacgaaaaca ggagaaatca tttcggcagt acatattgaa
      721 gcgtatatag gacgagtaac tgtttgtgca gaagccattg cgattggtag tgcagtttcg
      781 aatggacaaa aggattttga cacgattgta gctgttagac acccttattc tgacgaagta
      841 gatagaagta ttcgagtggt aagtccttgt ggtatgtgta gggagtttga tttcagacta
      901 tgcaccagat tgttttgtaa a
//
