LOCUS       sequence-364910-       11670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral delivery of recombinase landing pad .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT,
            Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD
  TITLE     Systematic discovery of recombinases for efficient integration of 
            large DNA sequences into the human genome.
  JOURNAL   Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 
            10.1038/s41587-022-01494-w.
  PUBMED    36217031
REFERENCE   2  (bases 1 to 11670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 
            10.1038/s41587-022-01494-w."
COMMENT     Sequence Label: pJT305 Pa01 Lenti  pEF-attB LSR GFP
FEATURES             Location/Qualifiers
     source          1..11670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2682..3860
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2913..3851
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3808..3828
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3968..3977
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5567..5620
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5621..5623,5624..6337)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5621..5623,5624..6334)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5663..5684)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5924..5943)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6271..6292
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6362..6950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6415..6435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6833..6844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6953..6969)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6954..6970)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7475..7655
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7681..7698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7687..7911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7957..8385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8044..8063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8254..8275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8394..8414)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8399..8728
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8579..8714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8641..8660
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8776..8823
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8842..9216
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9346..9467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9383..9402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9437..9456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9516..9532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9516..9532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9529..9551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9540..9556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9564..9570,9571..9588,9589..9594))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9609..9630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9747..9764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9918..10506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9998..10017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10677..11468,11469..11537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11300..11319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11538..11642)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     3001 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3061 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3121 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3181 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3241 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3301 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3361 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3421 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3481 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3541 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3601 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3661 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3721 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3781 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3841 ttccatttca ggtgtcgtga ccggtttccc ttcgcacccg caccgcggct tcgagaccgt
     3901 gacctacatg ctcgaagggc gtatgcgcca cgaagaccac ctcggcaatc gcggcctgct
     3961 caaggccgcc accatgggcc ctagcgcctt cagctacgtg cgctttagca gcggcaagca
     4021 ggccaagggc agctctgaac acagacagag agccatgctg ggacagtggc tggaacagca
     4081 ccctagcttc acactgagtg atctgcggtt cgaggacctg ggcagaagcg gattcagcgg
     4141 cgagcacctg gatcacggcc tgggccagct gctggctgct atcgacagcg gcgccatcaa
     4201 gagcggagat gtgatcctgg tcgaggccgt ggacagaatc ggcagactgg agcctctgga
     4261 aatgctgcct ctgttctcca gaatcgtgaa ggccggcgtg tccgttatca ccctggaaga
     4321 tggccacgtt tacgaccgga gctccgtgaa cgagacatct ctgtttctgc tggtggccaa
     4381 aatccagcag gcccacgagt actctaatag actgtctcga agaatcaacg cctcttacac
     4441 cgctcggaga gaaaaagcta aagcaggcct gggcatcaag agagagacac ctgtgtggct
     4501 gaccacagac ggccaactgg tgcctcacgt ggcccctcac atcgcccagg cttttcagga
     4561 ctacgccgat ggcctgggag agagaaggat ttgtagaaag ctgcgggaga gcggcctgga
     4621 agaattctcc aagaccaacg ccaccaccgt gcggagatgg ctgaagaacc ggaccgccat
     4681 tggatattgg aacgacatcc ctgatgtgta ccctcatgtc gtggaccctg ctctgttcta
     4741 ccaggtgcag cagcgcctgg acgcccctaa ggtggacaga gccaagccca gcgctcacta
     4801 cctgacaggc ctcgtgaagt gcgccgtgtg cggcagaaac tacaactaca aacagcggaa
     4861 gcacaccgac cccgctatgc tgtgcaccag cagagccaga ctggccgggg aaggctgcag
     4921 caatagcaag acataccccg tgatagttct ggaccaggtg cggaagctga ccagcctgcc
     4981 gttcctgcaa cacgccatgg aatccgcctc atctcaggcc gacccaagca gccagagact
     5041 ggccgtgatc gacggcgaaa tcggcgagct gtccagaaag atcagcgagg ccacaaaggc
     5101 cctgctggtg ctgggcttca cccctgagat ccaggagagc ctggaacagc tcaagaccgc
     5161 cagagaagcc ctggaggaag aaagagccac cctgctgctg cctcaggccg agaagctgac
     5221 aacagctcag ctggaggcct tcagcaacgg cctgctggac gacgagccca tgaagctcaa
     5281 ccacgtgctt cagaccgccg gctacagcat ggtggtgcac cccgacggct ctatcgacgt
     5341 ggatggaaaa agattcgtgt acgagggcgc tagccggaaa gagaaagtgt acaagctgag
     5401 actgatcggc gaggataagc agtggagcct gcccatcctg accccacaga tggccactta
     5461 caagtccctg ttcatggccg ctgtgcggct gcctggcgac cccagcgagg aagagctgag
     5521 acggttcgag gaagccaagc acagcgagcg ggctagcggc agtggagagg gcagaggaag
     5581 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca gtgagcaagg gcgaggagct
     5641 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5701 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     5761 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     5821 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5881 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     5941 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     6001 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6061 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6121 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     6181 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     6241 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     6301 cgggatcact ctcggcatgg acgagctgta caagtaagaa ttcgatatca agcttatcga
     6361 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6421 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6481 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6541 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6601 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6661 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6721 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     6781 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6841 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6901 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     6961 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     7021 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     7081 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7141 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     7201 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     7261 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     7321 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     7381 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     7441 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     7501 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7561 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7621 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     7681 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     7741 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     7801 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     7861 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     7921 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     7981 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8041 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8101 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8161 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8221 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8281 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8341 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     8401 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8461 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8521 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8581 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8641 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     8701 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     8761 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     8821 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     8881 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     8941 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     9001 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     9061 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     9121 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     9181 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     9241 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9301 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     9361 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9421 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     9481 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9541 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9601 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9661 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9721 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    10381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    11041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    11161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    11221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    11461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11641 cgcacatttc cccgaaaagt gccacctgac
//
