LOCUS       sequence-364912-        9391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of Vesicle Nucleating peptide-mNeongreen and 
            cydAB-mCherry fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Eastwood TA, Baker K, Streather BR, Allen N, Wang L, Botchway SW, 
            Brown IR, Hiscock JR, Lennon C, Mulvihill DP
  TITLE     High-yield vesicle-packaged recombinant protein production from E. 
            coli.
  JOURNAL   Cell Rep Methods. 2023 Feb 2;3(2):100396. doi: 
            10.1016/j.crmeth.2023.100396. eCollection 2023 Feb 27.
  PUBMED    36936078
REFERENCE   2  (bases 1 to 9391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-02-2"; volume: "3(2):100396. doi"; pages: " 
            10.1016/j.crmeth.2023.100396. eCollection 2023 Feb 27"
COMMENT     Sequence Label: pETDuet-1_VNp-mNeongreen_CydAB-mCherry
FEATURES             Location/Qualifiers
     source          1..9391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     569..588
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(608..685)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     891..910
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        998..1016
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1017..1041
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1056..1078
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(3741..3759)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4009..4028
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        4371..4389
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4390..4414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4589..5296
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     CDS             5321..5338
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5354..5398
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(5436..5454)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5450..5497
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5534..5989
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5621..5640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5831..5852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(6104..6895,6896..6964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6727..6746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6965..7057)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7138..7726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7627..7646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7880..7897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7912..8051
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7977..7996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8137..8159
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8153..8344)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(8916..9391,1..607))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
       61 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
      121 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
      181 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
      241 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
      301 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
      361 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
      421 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
      481 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
      541 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
      601 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
      661 aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc
      721 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
      781 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
      841 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
      901 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
      961 gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac tataggggaa
     1021 ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata
     1081 taccatggag ttccagttcg ggactaactg gtcttactat tcccactatg taggggatat
     1141 cttcggtgcg ccgctggcaa tcgaaggtct gatggccttc ttcctcgaat ccacctttgt
     1201 aggtctgttc ttcttcggtt gggatcgtct gggtaaagtt cagcatatgt gtgtcacctg
     1261 gctggtggcg ctcggttcaa acctgtccgc actgtggatt ctggttgcga acggctggat
     1321 gcaaaaccca atcgcgtccg atttcaactt tgaaactatg cgtatggaga tggtgagctt
     1381 ctccgagctg gtgcttaacc cggttgctca ggtgaaattc gttcacactg tagcgtctgg
     1441 ttatgtgact ggcgcgatgt tcatcctcgg tatcagcgca tggtatatgc tgaaaggtcg
     1501 tgacttcgcc ttcgctaaac gctcctttgc tatcgctgcc agcttcggta tggctgctgt
     1561 tctgtctgtt attgttctgg gtgatgaatc cggctacgaa atgggcgacg tgcagaaaac
     1621 caaactggct gctattgaag ccgagtggga aacgcaacct gcgcctgctg cctttactct
     1681 gttcggcatt cctgatcagg aagaggagac gaacaaattt gcgattcaga tcccttacgc
     1741 actgggcatc attgcaacgc gttccgtgga taccccggtt atcggcctga aagagctgat
     1801 ggtgcagcat gaagaacgca ttcgtaacgg gatgaaggcg tactctctgc tcgaacaact
     1861 gcgttctggt tctaccgacc aggcggttcg tgaccagttc aatagcatga agaaagacct
     1921 cggttacggt ctgctgctga aacgctatac gccaaacgtg gctgatgcga ctgaagcgca
     1981 gattcaacag gcaaccaaag actccatccc gcgtgtagcg ccgctgtact ttgcgttccg
     2041 tatcatggtg gcgtgtggct tcctgcttct ggcaatcatc gcgctctctt tctggagtgt
     2101 catccgcaac cgcattggcg agaaaaaatg gcttctgcgc gccgcgctgt acggtattcc
     2161 gctgccgtgg attgctgtag aagcgggctg gttcgtggct gaatatggcc gccaaccgtg
     2221 ggctatcggt gaagtgctgc cgacagctgt ggcgaactcg tcactgaccg caggcgatct
     2281 catcttctca atggtgctga tttgcggcct gtataccctg ttcctggtgg cagaattgtt
     2341 cttaatgttc aagtttgcac gcctcggccc aagcagcctg aaaaccggtc gctatcactt
     2401 tgagcagtct tccacgacta ctcagccggc acgctaagac aggagtcgtc aaatgatcga
     2461 ttatgaagta ttgcgtttta tctggtggct gctggttggc gttctgctga ttggttttgc
     2521 agtcactgac ggtttcgaca tgggggtggg catgctcacc cgtttcctcg gtcgtaacga
     2581 caccgagcgt cgaattatga ttaactccat tgcaccacac tgggacggta accaggtttg
     2641 gctgatcacc gcgggcggcg cactctttgc tgcctggccg atggtctatg ccgctgcgtt
     2701 ctccggcttc tatgtggcga tgatcctcgt gctggcgtct ttgttcttcc gtccggtcgg
     2761 ttttgactac cgctccaaga ttgaagaaac ccgctggcgt aacatgtggg actggggcat
     2821 cttcattggt agcttcgttc cgccgctggt aattggtgta gcgttcggta acctgttgca
     2881 gggcgtaccg ttcaacgttg atgaatatct gcgtctgtac tacaccggta acttcttcca
     2941 gttgcttaac ccgttcggcc tgctggcagg cgtggtgagc gtagggatga tcattactca
     3001 gggcgcaacc tatctgcaaa tgcgtaccgt gggcgaactg cacctgcgta cccgtgcaac
     3061 ggctcaggtg gctgcgctgg tgacactggt ctgtttcgca ctggctggcg tatgggtgat
     3121 gtacggtatc gatggttatg tcgtgaaatc gacaatggac cattacgcag cctctaaccc
     3181 actgaataaa gaagtggttc gtgaagctgg cgcatggctg gttaacttca acaacacgcc
     3241 aattctgtgg gctattccgg cactgggtgt ggttctgccg ctgctgacca tcctgactgc
     3301 acgtatggat aaagccgcgt gggcgtttgt gttctcctcc ctgacgctgg cctgcatcat
     3361 cctgacagcc ggtatcgcaa tgttcccgtt tgtgatgccg tccagcacca tgatgaacgc
     3421 aagtctgaca atgtgggatg caacttccag ccagctgacg cttaacgtca tgacctgggt
     3481 tgcggtggtt ctggtaccga tcattctgct ctacaccgcc tggtgttact ggaaaatgtt
     3541 cggtcgtatc accaaagaag atattgaacg taacacccac tctctgtacg tcgacatggt
     3601 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3661 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3721 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3781 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcttacgtga agcaccccgc
     3841 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3901 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3961 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     4021 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4081 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4141 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4201 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4261 cgagggccgc cactccaccg gcggcatgga cgagtgtaca agtaagcggc cgcataatgc
     4321 ttaagtcgaa cagaaagtaa tcgtattgta cacggccgca taatcgaaat taatacgact
     4381 cactataggg gaattgtgag cggataacaa ttccccatct tagtatatta gttaagtata
     4441 agaaggagat atacatatgg atgtattcat gaaaggactt tcaaaggcca aggagggagt
     4501 tgtggcagct gctgagaaaa ccaaacaggg tgtggcagaa gcagcaggaa agacaaaaga
     4561 gggtgttctc agatctggag gaagcggaat ggtgagcaag ggcgaggagg ataacatggc
     4621 ctctctccca gcgacacatg agttacacat ctttggctcc atcaacggtg tggactttga
     4681 catggtgggt cagggcaccg gcaatccaaa tgatggttat gaggagttaa acctgaagtc
     4741 caccaagggt gacctccagt tctccccctg gattctggtc cctcatatcg ggtatggctt
     4801 ccatcagtac ctgccctacc ctgacgggat gtcgcctttc caggccgcga tggtagatgg
     4861 ctccggatac caagtccatc gcacaatgca gtttgaagat ggtgcctccc ttactgttaa
     4921 ctaccgctac acctacgagg gaagccacat caaaggagag gcccaggtga aggggactgg
     4981 tttccctgct gacggtcctg tgatgaccaa ctcgctgacc gctgcggact ggtgcaggtc
     5041 gaagaagact taccccaacg acaaaaccat catcagtacc tttaagtgga gttacaccac
     5101 tggaaatggc aagcgctacc ggagcactgc gcggaccacc tacacctttg ccaagccaat
     5161 ggcggctaac tatctgaaga accagccgat gtacgtgttc cgtaagacgg agctcaagca
     5221 ctccaagacc gagctcaact tcaaggagtg gcaaaaggcc tttaccgatg tgatgggcat
     5281 ggacgagctg tacaaggcta gcggaggaag cggaggtacc catcatcatc atcatcatta
     5341 actcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc
     5401 tactagcgca gcttaattaa cctaggctgc tgccaccgct gagcaataac tagcataacc
     5461 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
     5521 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5581 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5641 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5701 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5761 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5821 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5881 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5941 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc tggcggcacg
     6001 atggcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6721 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6961 tcatactctt cctttttcaa tcatgattga agcatttatc agggttattg tctcatgagc
     7021 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gtcatgacca aaatccctta
     7081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7261 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7801 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7861 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     7921 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca
     7981 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     8041 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8101 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8161 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     8221 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     8281 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     8341 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
     8401 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
     8461 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
     8521 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg
     8581 cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg
     8641 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
     8701 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
     8761 gccagcctag ccgggtcctc aacgacagga gcacgatcat gctagtcatg ccccgcgccc
     8821 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     8881 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8941 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9001 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     9061 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     9121 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     9181 tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     9241 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     9301 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     9361 atcggctgaa tttgattgcg agtgagatat t
//
