LOCUS       sequence-364927-        6730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human TRF2 with a zz affinity tag, YBBR labeling site, and
            TEV cleavage site in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Jack A, Kim Y, Strom AR, Lee DSW, Williams B, Schaub JM, Kellogg EH,
            Finkelstein IJ, Ferro LS, Yildiz A, Brangwynne CP
  TITLE     Compartmentalization of telomeres through DNA-scaffolded phase 
            separation.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 
            10.1016/j.devcel.2021.12.017.
  PUBMED    35077681
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "277-290.e9"
COMMENT     Sequence Label: pOmniBac zz TEV YBBR TRF2
FEATURES             Location/Qualifiers
     source          1..6730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(505..510,511..527,528..533))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(604..637)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        714..805
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     730..747
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             872..895
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             938..1111
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1112..1285
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             1343..1363
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1385..1405
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3201..3335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3238..3257)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3292..3311
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3456..3621
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_recomb     3628..4333
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3716..4088
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3745..4333)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      4523..5111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5012..5031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_recomb     5367..6194
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5367..5491
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5529..6134)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     mobile_element  6223..6446
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(6513..6730,1..316))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg
       61 cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt
      121 aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa
      181 gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga
      241 tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt
      301 tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
      361 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
      421 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
      481 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt
      541 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
      601 gccataactt cgtatagcat acattatacg aagttatctg taactataac ggtcctaagg
      661 tagcgagttt aaacactagt atcgattcgc gacctactcc ggaatattaa tagatcatgg
      721 agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag
      781 ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg
      841 cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacc atcacggttc
      901 tatgtgcatg gcaggccttg cgcaacacga tgaagccgtg gacaacaaat tcaacaaaga
      961 acaacaaaac gcgtactatg agatcttaca tttacctaac ttaaacgaag gacaacgaaa
     1021 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
     1081 taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca
     1141 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt
     1201 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctgaaaa
     1261 gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc gcggggaagt caaccgatta
     1321 tgatattcca actactgcta gcgagaacct atatttccag ggagattatg atatccctac
     1381 gactgagaat ctttattttc agggcatgga ctcattggag tttatcgcat caaagctcgc
     1441 aatggccgcg ggagccggga cggcgggccc cgcttccggc ccgggcgtcg tgcgtgaccc
     1501 agcggcgtca cagccgagga agcggcccgg ccgggagggc ggggagggcg cgcggcgatc
     1561 ggacacgatg gcgggaggag gcgggagtag cgacggcagc gggcgggcag ctggcaggcg
     1621 ggcgtcccgc agtagcgggc gggcccggcg ggggcgccac gagccggggc tggggggccc
     1681 ggcggagcgc ggcgcggggg aggcacggct ggaagaggca gtcaatcgct gggtgctcaa
     1741 gttctacttc cacgaggcgc tgcgggcctt tcggggtagc cggtacgggg acttcagaca
     1801 gatccgggac atcatgcagg ctttgcttgt caggcccttg gggaaggagc acaccgtgtc
     1861 ccgattgctg cgggttatgc agtgtctgtc gcggattgaa gaaggggaaa atttagactg
     1921 ttcctttgat atggaggctg agctcacacc actggaatca gctatcaatg tgctggagat
     1981 gattaaaacg gaatttacac tgacagaagc agtggtcgaa tccagtagaa aactggtcaa
     2041 ggaagctgct gtcattattt gtatcaaaaa caaagaattt gaaaaggctt caaaaatttt
     2101 gaaaaaacat atgtccaagg accccacaac tcagaagctg agaaatgatc tcctgaatat
     2161 tattcgagaa aagaacttgg cccatcctgt tatccagaac ttttcatatg agaccttcca
     2221 gcagaagatg ctgcgcttcc tggagagcca cctggatgac gccgagccct acctcctcac
     2281 gatggccaaa aaggctttga aatctgagtc cgctgcctca agtacaggga aggaagataa
     2341 acagccagca ccagggcctg tggaaaagcc acccagagaa cccgcaaggc agctacggaa
     2401 tcctccaacc accattggaa tgatgactct gaaagcagct ttcaagactc tgtctggtgc
     2461 acaggattct gaggcagcct ttgcaaaact ggaccagaag gatctggttc ttcctactca
     2521 agctctccca gcatcaccag ccctcaaaaa caagagaccc agaaaagatg aaaacgaaag
     2581 ttcagccccg gctgacggtg agggtggctc ggaactgcag cccaagaaca agcgcatgac
     2641 aataagcaga ttggtcttgg aggaggacag ccagagtact gagcccagcg caggcctcaa
     2701 ctcctcccag gaggccgctt cagcgccacc atccaagccc accgttctca accaacccct
     2761 ccctggagag aagaatccca aagtacccaa aggcaagtgg aacagctcta atggggttga
     2821 agaaaaggag acttgggtgg aagaggatga actgtttcaa gttcaggcac cagatgaaga
     2881 cagtacaacc aatataacaa aaaagcagaa gtggactgta gaagaaagcg agtgggtcaa
     2941 ggctggagtg cagaaatatg gggaaggaaa ctgggctgcc atttctaaaa attacccatt
     3001 tgttaaccga acagctgtga tgattaagga tcgctggcgg accatgaaaa gacttggcat
     3061 gaactgataa tgagcttgtc gagaagtact agaggatcat aatcagccat accacatttg
     3121 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3181 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3301 ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag cctagaagat
     3361 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     3421 ctatcataac ccctagggta tacccatcta attggaacca gataagtgaa atctagttcc
     3481 aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc
     3541 tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc
     3601 caactatttt gtccgcccac aaccggtgga ggaaattctc cttgaagttt ccctggtgtt
     3661 caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt attaaaacac
     3721 gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga
     3781 caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag
     3841 agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat
     3901 agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc
     3961 tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa
     4021 tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa
     4081 aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt
     4141 aaacacgtta aatagagctt ggacatattt aacatcgggc gtgttagctt tattaggccg
     4201 attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat
     4261 agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga
     4321 gctttttgga attaccggtt gacttgggtc aactgtcaga ccaagtttac tcatatatac
     4381 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4441 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4501 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4561 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4621 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4681 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4741 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4801 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4861 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4921 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4981 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5041 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5101 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5161 ttgctcacat gttctttcct gcgttatccc ctgattgact tgggtcgctc ttcctgtgga
     5221 tgcgcaggta tgtacaggaa gaggtttata ctaaactgtt acattgcaaa cgtggtttcg
     5281 tgtgccaagt gtgaaaaccg atgtttaatc aaggctctga cgcatttcta caaccacgac
     5341 tctaagtgtg tgggtgaagt catgcatgca tcttttaatc aaatcccaag atgtgtataa
     5401 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
     5461 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgtcaaattt
     5521 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
     5581 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
     5641 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
     5701 tcgtattcct tctctttttc atttttctct tcataaaaat taacatagtt attatcgtat
     5761 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
     5821 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
     5881 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
     5941 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
     6001 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     6061 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     6121 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     6181 aatatatagt tgctgattgc gcagatgccc tgcgtaagcg ggtgtgggcg gacaataaag
     6241 tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata
     6301 gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg
     6361 ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg
     6421 gccaagggca tgtaaagact atattcgcgg cgttgtgaca atttaccgaa caactccgcg
     6481 gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca
     6541 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca
     6601 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg
     6661 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca
     6721 tagatataga
//
