LOCUS       sequence-364930-        6415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human TRF1 with a zz affinity tag, YBBR labeling site, and
            TEV cleavage site in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Jack A, Kim Y, Strom AR, Lee DSW, Williams B, Schaub JM, Kellogg EH,
            Finkelstein IJ, Ferro LS, Yildiz A, Brangwynne CP
  TITLE     Compartmentalization of telomeres through DNA-scaffolded phase 
            separation.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 
            10.1016/j.devcel.2021.12.017.
  PUBMED    35077681
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "277-290.e9"
COMMENT     Sequence Label: pOmniBac zz TEV YBBR TRF1
FEATURES             Location/Qualifiers
     source          1..6415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  586..809
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(876..1409)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1598..1603,1604..1620,1621..1626))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1697..1730)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1807..1898
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1823..1840
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1965..1988
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             2031..2204
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2205..2378
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             2436..2456
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2478..2498
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3979..4113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4016..4035)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4070..4089
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4234..4399
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_recomb     4406..5111
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           4494..4866
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(4523..5111)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      5301..5889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5790..5809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_recomb     join(6145..6415,1..557)
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             6145..6269
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(join(6307..6415,1..497))
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 cagttaattt gcgacaatat aattttattt tcacataaac tagacgcctt gtcgtcttct
       61 tcttcgtatt ccttctcttt ttcatttttc tcttcataaa aattaacata gttattatcg
      121 tatccatata tgtatctatc gtatagagta aattttttgt tgtcataaat atatatgtct
      181 tttttaatgg ggtgtatagt accgctgcgc atagtttttc tgtaatttac aacagtgcta
      241 ttttctggta gttcttcgga gtgtgttgct ttaattatta aatttatata atcaatgaat
      301 ttgggatcgt cggttttgta caatatgttg ccggcatagt acgcagcttc ttctagttca
      361 attacaccat tttttagcag caccggatta acataacttt ccaaaatgtt gtacgaaccg
      421 ttaaacaaaa acagttcacc tcccttttct atactattgt ctgcgagcag ttgtttgttg
      481 ttaaaaataa cagccattgt aatgagacgc acaaactaat atcacaaact ggaaatgtct
      541 atcaatatat agttgctgat tgcgcagatg ccctgcgtaa gcgggtgtgg gcggacaata
      601 aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa actagacaga
      661 atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg acttttgtta
      721 tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc
      781 gtggccaagg gcatgtaaag actatattcg cggcgttgtg acaatttacc gaacaactcc
      841 gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata
      901 tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg
      961 tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg
     1021 aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga
     1081 tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga
     1141 gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc
     1201 aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac
     1261 tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta
     1321 catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc
     1381 tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     1441 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc
     1501 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     1561 ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta
     1621 tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
     1681 cagcagcgaa gtcgccataa cttcgtatag catacattat acgaagttat ctgtaactat
     1741 aacggtccta aggtagcgag tttaaacact agtatcgatt cgcgacctac tccggaatat
     1801 taatagatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
     1861 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
     1921 ccaccatcgg gcgcggatct cggtccgaaa ccatgtcgta ctaccatcac catcaccatc
     1981 accatcacgg ttctatgtgc atggcaggcc ttgcgcaaca cgatgaagcc gtggacaaca
     2041 aattcaacaa agaacaacaa aacgcgtact atgagatctt acatttacct aacttaaacg
     2101 aaggacaacg aaacgccttc atccaaagtt taaaagatga cccaagccaa agcgctaacc
     2161 ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac aacaaattca
     2221 acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta aacgaagaac
     2281 aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct aaccttttag
     2341 cagaagctga aaagctaaat gatgctcagg cgccgaaagt agacgcaaat tccgcgggga
     2401 agtcaaccga ttatgatatt ccaactactg ctagcgagaa cctatatttc cagggagatt
     2461 atgatatccc tacgactgag aatctttatt ttcagggcga ctcattggag tttatcgcat
     2521 caaagctcgc aatggcggag gatgtttcct cagcggcccc gagcccgcgg ggctgtgcgg
     2581 atggtaggga tgccgaccct actgaggagc agatggcaga aacagagaga aacgacgagg
     2641 agcagttcga atgccaggaa ctgctcgagt gccaggtgca ggtgggggcc cccgaggagg
     2701 aggaggagga ggaggaggac gcgggcctgg tggccgaggc cgaggccgtg gctgccggct
     2761 ggatgctcga tttcctctgc ctctctcttt gccgagcttt ccgcgacggc cgctccgagg
     2821 acttccgcag gacccgcaac agcgcagagg ctattattca tggactatcc agtctaacag
     2881 cttgccagtt gagaacgata tacatatgtc agtttttgac aagaattgca gcaggaaaaa
     2941 cccttgatgc acagtttgaa aatgatgaac gaattacacc cttggaatca gccctgatga
     3001 tttggggttc aattgaaaag gaacatgaca aacttcatga agaaatacag aatttaatta
     3061 aaattcaggc tatagctgtt tgtatggaaa atggcaactt taaagaagca gaagaagtct
     3121 ttgaaagaat atttggtgat ccaaattctc atatgccttt caaaagcaaa ttgcttatga
     3181 taatctctca gaaagataca tttcattcct tttttcaaca cttcagctac aaccacatga
     3241 tggagaaaat taagagttat gtgaattatg tgctaagtga aaaatcatca acctttctaa
     3301 tgaaggcagc ggcaaaagta gtagaaagca aaaggacaag aacaataact tctcaagata
     3361 aacctagtgg taatgatgtt gaaatggaaa ctgaagctaa tttggataca agaaaaagtg
     3421 ttagtgacaa acagtctgcg gtaactgaat cctcagaggg tacagtatcc ttattgaggt
     3481 ctcacaagaa tcttttctta tctaagttgc aacatggaac ccagcaacaa gaccttaata
     3541 agaaagaaag aagagtagga actcctcaaa gtacaaaaaa gaaaaaagaa agcagaagag
     3601 ccactgaaag cagaatacct gtttcaaaga gtcagccggt aactcctgaa aaacatcgag
     3661 ctagaaaaag acaggcatgg ctttgggaag aagacaagaa tttgagatct ggcgtgagga
     3721 aatatggaga gggaaactgg tctaaaatac tgttgcatta taaattcaac aaccggacaa
     3781 gtgtcatgtt aaaagacaga tggaggacca tgaagaaact aaaactgatt tcctcagaca
     3841 gcgaagactg agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta
     3901 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3961 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4081 aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc tagaagatcc
     4141 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     4201 atcataaccc ctagggtata cccatctaat tggaaccaga taagtgaaat ctagttccaa
     4261 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
     4321 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
     4381 actattttgt ccgcccacaa ccggtggagg aaattctcct tgaagtttcc ctggtgttca
     4441 aagtaaagga gtttgcacca gacgcacctc tgttcactgg tccggcgtat taaaacacga
     4501 tacattgtta ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca
     4561 attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag
     4621 cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag
     4681 atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg
     4741 gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc
     4801 tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa
     4861 tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa
     4921 acacgttaaa tagagcttgg acatatttaa catcgggcgt gttagcttta ttaggccgat
     4981 tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat tttgccatag
     5041 ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt gtagttgagc
     5101 tttttggaat taccggttga cttgggtcaa ctgtcagacc aagtttactc atatatactt
     5161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5941 gctcacatgt tctttcctgc gttatcccct gattgacttg ggtcgctctt cctgtggatg
     6001 cgcaggtatg tacaggaaga ggtttatact aaactgttac attgcaaacg tggtttcgtg
     6061 tgccaagtgt gaaaaccgat gtttaatcaa ggctctgacg catttctaca accacgactc
     6121 taagtgtgtg ggtgaagtca tgcatgcatc ttttaatcaa atcccaagat gtgtataaac
     6181 caccaaactg ccaaaaaatg aaaactgtcg acaagctctg tccgtttgct ggcaactgca
     6241 agggtctcaa tcctatttgt aattattgaa taataaaaca attataaatg tcaaatttgt
     6301 tttttattaa cgatacaaac caaacgcaac aagaacattt gtagtattat ctataattga
     6361 aaacgcgtag ttataatcgc tgaggtaata tttaaaatca ttttcaaatg attca
//
