LOCUS       sequence-36496-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(74..91)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             complement(110..130)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(188..211)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 cttactagcc accgcaacag ccaggacaac agctcccact accaatattg gattggaagt
      121 acaggttttc ctcgatcccg aggttgttgt tattgttatt gttgttgttg ttcgagctcg
      181 aattagtctg cgcgtctttc agggcttcat cgacagtctg acgaccgctg gcggcgttga
      241 tcaccgcagt acgcacggca taccagaaag cggacatctg cgggatgttc ggcatgattt
      301 cacctttctg ggcgttttcc atagtggcgg caatacgtgg atctttcgcc aactcttcct
      361 cgtaagactt cagcgctacg gcacccagcg gtttgtcttt attaaccgct tccagacctt
      421 catcagtcag cagatagttt tcgaggaact cttttgccag ctctttgttc ggactggcgg
      481 cgttaatacc tgcgctcagc acgccaacga acggtttgga tggttgaccc ttgaaggtcg
      541 gcagtaccgt tacaccataa ttcactttgc tggtgtcgat gttggaccat gcccacgggc
      601 cgttgatggt catcgctgtt tcgcctttat taaaggcagc ttctgcgatg gagtaatcgg
      661 tgtctgcatt catgtgtttg tttttaatca ggtcaaccag gaaggtcaga cccgctttcg
      721 cgccagcgtt atccacgccc acgtctttaa tgtcgtactt gccgttttca tacttgaacg
      781 cataaccccc gtcagcagca atcagcggcc aggtgaagta cggttcttgc aggttgaaca
      841 tcagcgcgct cttacctttc gctttcagtt ctttatccag cgccgggatc tcttcccagg
      901 tttttggcgg gttcggcagc agatctttgt tataaatcag cgataacgct tcaacagcga
      961 tcgggtaagc aatcagcttg ccgt
//
