LOCUS       sequence-364960-         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human ATG13 HORMA W50D mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Ren X, Nguyen TN, Lam WK, Buffalo CZ, Lazarou M, Yokom AL, Hurley JH
  TITLE     Structural basis for ATG9A recruitment to the ULK1 complex in 
            mitophagy initiation.
  JOURNAL   Sci Adv. 2023 Feb 15;9(7):eadg2997. doi: 10.1126/sciadv.adg2997. 
            Epub 2023 Feb 15.
  PUBMED    36791199
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-02-15"; volume: "9(7):eadg2997. doi"; pages: " 
            10.1126/sciadv.adg2997. Epub 2023 Feb 15"
COMMENT     Sequence Label: pCAG-OSF-ATG13 (2-197)-W50D
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             524..535
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gagaccgacc tcaacagcca ggaccgtaaa gacctcgaca agttcattaa gtttttcgcc
       61 ctcaagaccg tgcaggtgat cgtgcaggcc cggctgggag agaaaatctg cacacgcagc
      121 tcctcttcac ccaccggttc tgacgatttc aacctggcta ttaaggatat ccctgaagtg
      181 actcacgaag caaagaaagc actcgctgga cagctgccag cagtgggtcg ttcaatgtgt
      241 gtcgagatct ccctgaagac atctgaagga gactcaatgg agctcgaaat ttggtgcctg
      301 gagatgaacg aaaagtgtga taaagagatc aaagtgagct acaccgtcta taatcgtctg
      361 tccctgctcc tgaaatctct cctggccatc actcgggtga cacctgctta ccgcctcagt
      421 aggaagcagg gtcatgaata tgtgattctg tacaggatct atttcggcga ggtccagctc
      481 tccggactgg gtgaaggctt tcagactgtg cgtgtcggta ccgtgggaac tccagtcggc
      541 acaattaccc tctcttgcgc ataccgtatc aatctggcct tcatgtcata a
//
