LOCUS       sequence-36497-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pKEB31
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(145..150,151..168,169..175)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(295..314)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
       61 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
      121 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
      181 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      241 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      301 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      361 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      421 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
      481 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
      541 ctctggagtg aataccacga cgatttccgg cagtttctac acatataatt cgcaagatgt
      601 ggcgtgttac ggtgaaaacc tgg
//
