LOCUS       sequence-364972-       11194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coexpresses human TPP1 with a zz affinity tag, YBBR labeling site, 
            and TEV cleavage site and human TIN2 with an MBP affinity tag and 
            TEV cleavage site in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Jack A, Kim Y, Strom AR, Lee DSW, Williams B, Schaub JM, Kellogg EH,
            Finkelstein IJ, Ferro LS, Yildiz A, Brangwynne CP
  TITLE     Compartmentalization of telomeres through DNA-scaffolded phase 
            separation.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 
            10.1016/j.devcel.2021.12.017.
  PUBMED    35077681
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "277-290.e9"
COMMENT     Sequence Label: pBig1a zz TEV YBBR TPP1 MBP TEV TIN2
FEATURES             Location/Qualifiers
     source          1..11194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             442..1233
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1274..1728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1361..1380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1570..1591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1754..1858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1859..1927,1928..2719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2077..2096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2890..3478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3379..3398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3632..3649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3783..4007
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4074..4607)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4796..4801,4802..4818,4819..4824))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(5220..5253)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5299..5318)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5343..5391
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5486..5577
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             5657..5830
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             5831..6004
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             6062..6082
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6104..6124
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    7664..7798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7954..8045
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             8101..8118
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8128..9228
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     9202..9225
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             9283..9303
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    10498..10632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  10947..11112
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgggaggca gaataaatga tcatatcgtc
      121 aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac
      181 ggtcacactg cttccggtag tcaataaacc ggtaagtagc gtatgcgctc acgcaactgg
      241 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      301 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
      361 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
      421 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
      481 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
      541 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
      601 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
      661 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
      721 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
      781 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
      841 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
      901 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
      961 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     1021 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     1081 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     1141 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     1201 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgg
     1261 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1561 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1621 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1681 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     1741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3001 aactggcttc agcagagcgc agataccaaa tactattctt ctagtgtagc cgtagttagg
     3061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3661 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     3721 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     3781 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     3841 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     3901 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     3961 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     4021 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     4081 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     4141 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     4201 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
     4261 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     4321 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     4381 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     4441 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     4501 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     4561 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca
     4621 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     4681 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     4741 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     4801 acgaaccgaa caggcttatg tcaactgggt ttgtgccttc atccgtttcc acggtgtgcg
     4861 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga
     4921 gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg
     4981 caaggtgctg tgcacggatc tgcccttgct tcaggagatc ggtagacctc ggccgtcgcg
     5041 gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg
     5101 cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc tacagctttg
     5161 tttgtactat caacaggttg aactgctgat caacagatcc tctacgcggc cgcggtacca
     5221 taacttcgta tagcatacat tatacgaagt tatctgggtt taaacgctct atggtctaaa
     5281 gtttttatcg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5341 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     5401 gggttcggtc ccagggctgg cactctgtca agtgaacgct ctatggtcta aagatttaaa
     5461 tcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
     5521 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
     5581 ccggattatt cataccgtcc caccatcggg cgcggatcca atgtgcatgg caggccttgc
     5641 gcaacacgat gaagccgtgg acaacaaatt caacaaagaa caacaaaacg cgtactatga
     5701 gatcttacat ttacctaact taaacgaagg acaacgaaac gccttcatcc aaagtttaaa
     5761 agatgaccca agccaaagcg ctaacctttt agcagaagct aaaaagctaa atgatgctca
     5821 ggcgccgaaa gtagacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt
     5881 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga
     5941 cccaagccaa agcgctaacc ttttagcaga agctgaaaag ctaaatgatg ctcaggcgcc
     6001 gaaagtagac gcaaattccg cggggaagtc aaccgattat gatattccaa ctactgctag
     6061 cgagaaccta tatttccagg gagattatga tatccctacg actgagaatc tttattttca
     6121 gggcgactca ttggagttta tcgcatcaaa gctcgcaatg gcaggttcgg ggaggctggt
     6181 cctacggccc tggattcggg agctgattct ggggtcagag acaccctcca gtccacgagc
     6241 cgggcagctg cttgaggtac tacaggacgc cgaggccgcg gtcgcgggcc catcccacgc
     6301 ccctgatacg tccgacgtcg gggccacgct gcttgtgtct gacgggaccc acagtgtccg
     6361 atgcctggtg acgcgggagg ccctggacac ctcggactgg gaggagaagg agttcggctt
     6421 ccgcgggaca gagggccggc tgctgctgct gcaggactgc ggggttcatg tccaggtcgc
     6481 tgagggcggc gcgcccgcag agttctatct ccaggtggac cgcttcagcc tgctgcccac
     6541 ggagcagccc cggctacggg tgcctggttg caaccaagac ttagatgttc agaaaaagct
     6601 ctatgactgc cttgaggagc acctttcaga gtccacctcg tccaatgcag gcctatcact
     6661 gtcccagctt ctggatgaaa tgcgggagga ccaggagcat cagggggcac tcgtgtgcct
     6721 ggctgaaagc tgcctgacac tggagggccc ttgcacagca ccccctgtca cccactgggc
     6781 tgcctcacga tgcaaggcca cgggagaagc tgtgtacact gtccccagct caatgctgtg
     6841 catctctgag aatgaccagc taattctgag ctctctaggc ccctgtcaga ggacacaggg
     6901 ccctgagctg cccccaccag acccggctct gcaggaccta tctctgaccc tcatagcctc
     6961 tcctccttcc tcacccagtt cctcaggaac cccggcctta cccggccaca tgtcatccga
     7021 ggaaagtggt accagcatca gccttctgcc tgccctgtcc ttggctgctc cagacccagg
     7081 gcagagaagc agctcccagc cctcaccagc catctgctca gcccctgcca ccctgacccc
     7141 caggtcccca cacgccagcc gtacccccag ctccccactc cagagctgca ctcccagtct
     7201 ctcaccccgt agccatgtcc ccagtccaca ccaggctctt gtgaccaggc cccagaaacc
     7261 tagcctggag ttcaaggagt ttgtagggtt gccctgcaag aatcggccgc cttttcccag
     7321 gaccggagct accaggggag cccaggagcc ctgctctgtc tgggaacccc caaagaggca
     7381 tcgtgatggt tctgccttcc agtatgagta tgagccaccc tgcacgtccc tctgtgctcg
     7441 ggtccaagct gtcaggcttc ctccccagct catggcctgg gccttgcact ttctgatgga
     7501 tgcacagcca gggtctgagc caactccgat gtgaaagctt gtcgagaagt actagaggat
     7561 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     7621 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     7681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     7801 atcactgctt gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     7861 ctgctgccac cgctgagcaa taactatcat aaccatttaa ataaacactg acattgactt
     7921 ggtttaaatc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     7981 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     8041 taaatattcc ggattattca taccgtccca ccatcgggcg cggatccaat gggttcttct
     8101 caccatcacc atcaccatgg ttcttctatg aaaatcgaag aaggtaaact ggtaatctgg
     8161 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     8221 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     8281 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     8341 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     8401 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     8461 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     8521 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     8581 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     8641 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     8701 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     8761 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     8821 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     8881 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     8941 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     9001 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     9061 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     9121 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     9181 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     9241 aacaacaaca acaataacaa taacaacaac ctcgggatcg aggaaaacct gtacttccaa
     9301 tccgctacgc ccctggtggc gggtcccgca gctctacgct tcgccgccgc ggctagctgg
     9361 caggttgtgc gcggacgctg cgtggaacat tttccgcgag tactggagtt tctgcgatct
     9421 ctgcgcgctg ttgcccctgg cttggttcgc taccggcacc acgaacgcct ttgtatgggc
     9481 ctaaaggcca aggtggtggt ggagctgatc ctgcagggcc ggccttgggc ccaagtcctg
     9541 aaagccctga atcaccactt tccagaatct ggacctatag tgcgggatcc caaggctaca
     9601 aagcaggatc tgaggaagat tttggaggca caggaaactt tttaccagca ggtgaagcag
     9661 ctgtcagagg ctcctgtgga tttggcctcg aagctgcagg aacttgaaca agagtatggg
     9721 gaaccctttc tggctgccat ggaaaagctg ctttttgagt acttgtgtca gctggagaaa
     9781 gcactgccta caccgcaggc acagcagctt caggatgtgc tgagttggat gcagcctgga
     9841 gtctctatca cctcttctct tgcctggaga caatatggtg tggacatggg gtggctgctt
     9901 ccagagtgct ctgttactga ctcagtgaac ctggctgagc ccatggaaca gaatcctcct
     9961 cagcaacaaa gactagcact ccacaatccc ctgccaaaag ccaagcctgg cacacatctt
    10021 cctcagggac catcttcaag gacgcaccca gaacctctag ctggccgaca cttcaatctg
    10081 gcccctctag gccgacgaag agttcagtcc caatgggcct ccactagggg aggccataag
    10141 gagcgcccca cagtcatgct gtttcccttt aggaatctcg gctcaccaac ccaggtcata
    10201 tctaagcctg agagcaagga agaacatgcg atatacacag cagacctagc catgggcaca
    10261 agagcagcct ccactgggaa gtctaagagt ccatgccaga ccctgggggg aagggctctg
    10321 aaggagaacc cagttgactt gcctgccaca gagcaaaagg agtaatgaaa gcttgtcgag
    10381 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
    10441 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    10501 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10561 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10621 catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca aagcccgaaa
    10681 ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataaccat ttaaataaat
    10741 ctatatctca atcggggtta cgtcgatacc ccaccgagac cccattggga ccaatacgcc
    10801 cgcgtttctt ccttttcccc accccaaccc ccaagttcgg gtgaaggccc agggctcgca
    10861 gccaacgtcg gggcggcaag ccctgccata gccactacgg gttaattaaa aactatatct
    10921 caatcggggt ttaaacagga gatccgaacc agataagtga aatctagttc caaactattt
    10981 tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc
    11041 actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt
    11101 tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa
    11161 ctccatgtga caaaccgtca tcttcggcta cttt
//
