LOCUS       sequence-364976-       17557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV 
            site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1
            each with an MBP affinity tag and TEV site in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17557)
  AUTHORS   Jack A, Kim Y, Strom AR, Lee DSW, Williams B, Schaub JM, Kellogg EH,
            Finkelstein IJ, Ferro LS, Yildiz A, Brangwynne CP
  TITLE     Compartmentalization of telomeres through DNA-scaffolded phase 
            separation.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 
            10.1016/j.devcel.2021.12.017.
  PUBMED    35077681
REFERENCE   2  (bases 1 to 17557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "277-290.e9"
COMMENT     Sequence Label: pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV T...
FEATURES             Location/Qualifiers
     source          1..17557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             442..1233
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1274..1728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1361..1380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1570..1591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1754..1858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1859..1927,1928..2719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2077..2096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2890..3478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3379..3398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3632..3649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3783..4007
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4074..4607)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4796..4801,4802..4818,4819..4824))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(5220..5253)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5299..5318)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5343..5391
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5486..5577
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             5657..5830
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             5831..6004
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             6062..6082
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6104..6124
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    8195..8329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        8485..8576
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             8632..8649
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8659..9759
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             9814..9834
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    11341..11475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        11631..11722
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             11778..11795
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11805..12905
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             12960..12980
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    14175..14309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        14465..14556
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             14636..14809
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             14810..14983
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             15041..15061
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             15083..15103
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    16861..16995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  17310..17475
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgggaggca gaataaatga tcatatcgtc
      121 aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac
      181 ggtcacactg cttccggtag tcaataaacc ggtaagtagc gtatgcgctc acgcaactgg
      241 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      301 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
      361 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
      421 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
      481 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
      541 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
      601 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
      661 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
      721 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
      781 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
      841 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
      901 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
      961 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     1021 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     1081 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     1141 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     1201 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgg
     1261 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1561 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1621 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1681 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     1741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3001 aactggcttc agcagagcgc agataccaaa tactattctt ctagtgtagc cgtagttagg
     3061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3661 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     3721 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     3781 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     3841 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     3901 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     3961 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     4021 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     4081 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     4141 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     4201 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
     4261 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     4321 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     4381 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     4441 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     4501 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     4561 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca
     4621 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     4681 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     4741 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     4801 acgaaccgaa caggcttatg tcaactgggt ttgtgccttc atccgtttcc acggtgtgcg
     4861 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga
     4921 gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg
     4981 caaggtgctg tgcacggatc tgcccttgct tcaggagatc ggtagacctc ggccgtcgcg
     5041 gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg
     5101 cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc tacagctttg
     5161 tttgtactat caacaggttg aactgctgat caacagatcc tctacgcggc cgcggtacca
     5221 taacttcgta tagcatacat tatacgaagt tatctgggtt taaacgctct atggtctaaa
     5281 gtttttatcg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5341 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     5401 gggttcggtc ccagggctgg cactctgtca agtgaacgct ctatggtcta aagatttaaa
     5461 tcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
     5521 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
     5581 ccggattatt cataccgtcc caccatcggg cgcggatcca atgtgcatgg caggccttgc
     5641 gcaacacgat gaagccgtgg acaacaaatt caacaaagaa caacaaaacg cgtactatga
     5701 gatcttacat ttacctaact taaacgaagg acaacgaaac gccttcatcc aaagtttaaa
     5761 agatgaccca agccaaagcg ctaacctttt agcagaagct aaaaagctaa atgatgctca
     5821 ggcgccgaaa gtagacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt
     5881 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga
     5941 cccaagccaa agcgctaacc ttttagcaga agctgaaaag ctaaatgatg ctcaggcgcc
     6001 gaaagtagac gcaaattccg cggggaagtc aaccgattat gatattccaa ctactgctag
     6061 cgagaaccta tatttccagg gagattatga tatccctacg actgagaatc tttattttca
     6121 gggcgactca ttggagttta tcgcatcaaa gctcgcaatg tctttggttc cagcaacaaa
     6181 ttatatatat acacccctga atcaacttaa gggtggtaca attgtcaatg tctatggtgt
     6241 tgtgaagttc tttaagcccc catatctaag caaaggaact gattattgct cagttgtaac
     6301 tattgtggac cagacaaatg taaaactaac ttgcctgctc tttagtggaa actatgaagc
     6361 ccttccaata atttataaaa atggagatat tgttcgcttt cacaggctga agattcaagt
     6421 atataaaaag gagactcagg gtatcaccag ctctggcttt gcatctttga cgtttgaggg
     6481 aactttggga gcccctatca tacctcgcac ttcaagcaag tattttaact tcactactga
     6541 ggaccacaaa atggtagaag ccttacgtgt ttgggcatct actcatatgt caccgtcttg
     6601 gacattacta aaattgtgtg atgttcagcc aatgcagtat tttgacctga cttgtcagct
     6661 cttgggcaaa gcagaagtgg acggagcatc atttcttcta aaggtatggg atggcaccag
     6721 gacaccattt ccatcttgga gagtcttaat acaagacctt gttcttgaag gtgatttaag
     6781 tcacatccat cggctacaaa atctgacaat agacatttta gtctacgata accatgttca
     6841 tgtggcaaga tctctgaagg ttggaagctt tcttagaatc tatagccttc ataccaaact
     6901 tcaatcaatg aattcagaga atcagacaat gttaagttta gagtttcatc ttcatggagg
     6961 taccagttac ggtcggggaa tcagggtctt gccagaaagt aactctgatg tggatcaact
     7021 gaaaaaggat ttagaatctg caaatttgac agccaatcag cattcagatg ttatctgtca
     7081 atcagaacct gacgacagct ttccaagctc tggatcagta tcattatacg aggtagaaag
     7141 atgtcaacag ctatctgcta caatacttac agatcatcag tatttggaga ggacaccact
     7201 atgtgccatt ttgaaacaaa aagctcctca acaataccgc atccgagcaa aattgaggtc
     7261 atataagccc agaagactat ttcagtctgt taaacttcat tgccctaaat gtcatttgct
     7321 gcaagaagtt ccacatgagg gcgatttgga tataattttt caggatggtg caactaaaac
     7381 cccagatgtc aagctacaaa atacatcatt atatgattca aaaatctgga ccactaaaaa
     7441 tcaaaaagga cgaaaagtag cagttcattt tgtgaaaaat aatggtattc tcccgctttc
     7501 aaatgaatgt ctacttttga tagaaggagg tacactcagt gaaatttgca aactctcgaa
     7561 caagtttaat agtgtaattc ctgtgagatc tggccacgaa gacctggaac ttttggacct
     7621 ttcagcacca tttcttatac aaggaacaat acatcactat ggatgtaaac agtgttctag
     7681 tttgagatcc atacaaaatc taaattccct ggttgataaa acatcgtgga ttccttcttc
     7741 tgtggcagaa gcactgggta ttgtacccct ccaatatgtg tttgttatga cctttacact
     7801 tgatgatgga acaggagtac tagaagccta tctcatggat tctgacaaat tcttccagat
     7861 tccagcatca gaagttctga tggatgatga ccttcagaaa agtgtggata tgatcatgga
     7921 tatgttttgt cctccaggaa taaaaattga tgcatatccg tggttggaat gcttcatcaa
     7981 gtcatacaat gtcacaaatg gaacagataa tcaaatttgc tatcagattt ttgacaccac
     8041 agttgcagaa gatgtaatct aatgaaagct tgtcgagaag tactagagga tcataatcag
     8101 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     8161 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     8221 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8281 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct
     8341 tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     8401 ccgctgagca ataactatca taaccattta aataaactgg atactattgc acgtttaaat
     8461 cgacctactc cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa
     8521 ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc
     8581 cggattattc ataccgtccc accatcgggc gcggatccaa tgggttcttc tcaccatcac
     8641 catcaccatg gttcttctat gaaaatcgaa gaaggtaaac tggtaatctg gattaacggc
     8701 gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt
     8761 aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tcccacaggt tgcggcaact
     8821 ggcgatggcc ctgacattat cttctgggca cacgaccgct ttggtggcta cgctcaatct
     8881 ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc
     8941 tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta
     9001 tcgctgattt ataacaaaga tctgctgccg aacccgccaa aaacctggga agagatcccg
     9061 gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa
     9121 ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac
     9181 ggcaagtacg acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc
     9241 ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca
     9301 gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc
     9361 aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa
     9421 ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccaaacaaa
     9481 gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt
     9541 aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa
     9601 gatccacgta ttgccgccac tatggaaaac gcccagaaag gtgaaatcat gccgaacatc
     9661 ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt
     9721 cgtcagactg tcgatgaagc cctgaaagac gcgcagacta attcgagctc gaacaacaac
     9781 aacaataaca ataacaacaa cctcgggatc gaggaaaacc tgtacttcca atccgcaggt
     9841 tcggggaggc tggtcctacg gccctggatt cgggagctga ttctggggtc agagacaccc
     9901 tccagtccac gagccgggca gctgcttgag gtactacagg acgccgaggc cgcggtcgcg
     9961 ggcccatccc acgcccctga tacgtccgac gtcggggcca cgctgcttgt gtctgacggg
    10021 acccacagtg tccgatgcct ggtgacgcgg gaggccctgg acacctcgga ctgggaggag
    10081 aaggagttcg gcttccgcgg gacagagggc cggctgctgc tgctgcagga ctgcggggtt
    10141 catgtccagg tcgctgaggg cggcgcgccc gcagagttct atctccaggt ggaccgcttc
    10201 agcctgctgc ccacggagca gccccggcta cgggtgcctg gttgcaacca agacttagat
    10261 gttcagaaaa agctctatga ctgccttgag gagcaccttt cagagtccac ctcgtccaat
    10321 gcaggcctat cactgtccca gcttctggat gaaatgcggg aggaccagga gcatcagggg
    10381 gcactcgtgt gcctggctga aagctgcctg acactggagg gcccttgcac agcaccccct
    10441 gtcacccact gggctgcctc acgatgcaag gccacgggag aagctgtgta cactgtcccc
    10501 agctcaatgc tgtgcatctc tgagaatgac cagctaattc tgagctctct aggcccctgt
    10561 cagaggacac agggccctga gctgccccca ccagacccgg ctctgcagga cctatctctg
    10621 accctcatag cctctcctcc ttcctcaccc agttcctcag gaaccccggc cttacccggc
    10681 cacatgtcat ccgaggaaag tggtaccagc atcagccttc tgcctgccct gtccttggct
    10741 gctccagacc cagggcagag aagcagctcc cagccctcac cagccatctg ctcagcccct
    10801 gccaccctga cccccaggtc cccacacgcc agccgtaccc ccagctcccc actccagagc
    10861 tgcactccca gtctctcacc ccgtagccat gtccccagtc cacaccaggc tcttgtgacc
    10921 aggccccaga aacctagcct ggagttcaag gagtttgtag ggttgccctg caagaatcgg
    10981 ccgccttttc ccaggaccgg agctaccagg ggagcccagg agccctgctc tgtctgggaa
    11041 cccccaaaga ggcatcgtga tggttctgcc ttccagtatg agtatgagcc accctgcacg
    11101 tccctctgtg ctcgggtcca agctgtcagg cttcctcccc agctcatggc ctgggccttg
    11161 cactttctga tggatgcaca gccagggtct gagccaactc cgatgtaatg aaagcttgtc
    11221 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
    11281 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    11341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11461 tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta acaaagcccg
    11521 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac catttaaata
    11581 aacctaatga tgcctgatgt ttaaatcgac ctactccgga atattaatag atcatggaga
    11641 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
    11701 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
    11761 atccaatggg ttcttctcac catcaccatc accatggttc ttctatgaaa atcgaagaag
    11821 gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta
    11881 agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag
    11941 agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg
    12001 accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt
    12061 tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg
    12121 cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc
    12181 cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga
    12241 gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg
    12301 ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata
    12361 acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga
    12421 atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga
    12481 ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa
    12541 cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag
    12601 gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc
    12661 tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga
    12721 agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccactatg gaaaacgccc
    12781 agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta
    12841 ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc
    12901 agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc gggatcgagg
    12961 aaaacctgta cttccaatcc gctacgcccc tggtggcggg tcccgcagct ctacgcttcg
    13021 ccgccgcggc tagctggcag gttgtgcgcg gacgctgcgt ggaacatttt ccgcgagtac
    13081 tggagtttct gcgatctctg cgcgctgttg cccctggctt ggttcgctac cggcaccacg
    13141 aacgcctttg tatgggccta aaggccaagg tggtggtgga gctgatcctg cagggccggc
    13201 cttgggccca agtcctgaaa gccctgaatc accactttcc agaatctgga cctatagtgc
    13261 gggatcccaa ggctacaaag caggatctga ggaagatttt ggaggcacag gaaacttttt
    13321 accagcaggt gaagcagctg tcagaggctc ctgtggattt ggcctcgaag ctgcaggaac
    13381 ttgaacaaga gtatggggaa ccctttctgg ctgccatgga aaagctgctt tttgagtact
    13441 tgtgtcagct ggagaaagca ctgcctacac cgcaggcaca gcagcttcag gatgtgctga
    13501 gttggatgca gcctggagtc tctatcacct cttctcttgc ctggagacaa tatggtgtgg
    13561 acatggggtg gctgcttcca gagtgctctg ttactgactc agtgaacctg gctgagccca
    13621 tggaacagaa tcctcctcag caacaaagac tagcactcca caatcccctg ccaaaagcca
    13681 agcctggcac acatcttcct cagggaccat cttcaaggac gcacccagaa cctctagctg
    13741 gccgacactt caatctggcc cctctaggcc gacgaagagt tcagtcccaa tgggcctcca
    13801 ctaggggatg ccataaggag cgccccacag tcatgctgtt tccctttagg aatctcggct
    13861 caccaaccca ggtcatatct aagcctgaga gcaaggaaga acatgcgata tacacagcag
    13921 acctagccat gggcacaaga gcagcctcca ctgggaagtc taagagtcca tgccagaccc
    13981 tggggggaag ggctctgaag gagaacccag ttgacttgcc tgccacagag caaaaggagt
    14041 aatgaaagct tgtcgagaag tactagagga tcataatcag ccataccaca tttgtagagg
    14101 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
    14161 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    14221 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    14281 tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctaga agatccggct
    14341 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactatca
    14401 taaccattta aataaacggt tcacatagct tagtttaaat cgacctactc cggaatatta
    14461 atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
    14521 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
    14581 accatcgggc gcggatccaa tgtgcatggc aggccttgcg caacacgatg aagccgtgga
    14641 caacaaattc aacaaagaac aacaaaacgc gtactatgag atcttacatt tacctaactt
    14701 aaacgaagga caacgaaacg ccttcatcca aagtttaaaa gatgacccaa gccaaagcgc
    14761 taacctttta gcagaagcta aaaagctaaa tgatgctcag gcgccgaaag tagacaacaa
    14821 attcaacaaa gaacaacaaa acgcgttcta tgagatctta catttaccta acttaaacga
    14881 agaacaacga aacgccttca tccaaagttt aaaagatgac ccaagccaaa gcgctaacct
    14941 tttagcagaa gctgaaaagc taaatgatgc tcaggcgccg aaagtagacg caaattccgc
    15001 ggggaagtca accgattatg atattccaac tactgctagc gagaacctat atttccaggg
    15061 agattatgat atccctacga ctgagaatct ttattttcag ggcatggccg cgggagccgg
    15121 gacggcgggc cccgcttccg gcccgggcgt cgtgcgtgac ccagcggcgt cacagccgag
    15181 gaagcggccc ggccgggagg gcggggaggg cgcgcggcga tcggacacga tggcgggagg
    15241 aggcgggagt agcgacggca gcgggcgggc agctggcagg cgggcgtccc gcagtagcgg
    15301 gcgggcccgg cgggggcgcc acgagccggg gctggggggc ccggcggagc gcggcgcggg
    15361 ggaggcacgg ctggaagagg cagtcaatcg ctgggtgctc aagttctact tccacgaggc
    15421 gctgcgggcc tttcggggta gccggtacgg ggacttcaga cagatccggg acatcatgca
    15481 ggctttgctt gtcaggccct tggggaagga gcacaccgtg tcccgattgc tgcgggttat
    15541 gcagtgtctg tcgcggattg aagaagggga aaatttagac tgttcctttg atatggaggc
    15601 tgagctcaca ccactggaat cagctatcaa tgtgctggag atgattaaaa cggaatttac
    15661 actgacagaa gcagtggtcg aatccagtag aaaactggtc aaggaagctg ctgtcattat
    15721 ttgtatcaaa aacaaagaat ttgaaaaggc ttcaaaaatt ttgaaaaaac atatgtccaa
    15781 ggaccccaca actcagaagc tgagaaatga tctcctgaat attattcgag aaaagaactt
    15841 ggcccatcct gttatccaga acttttcata tgagaccttc cagcagaaga tgctgcgctt
    15901 cctggagagc cacctggatg acgccgagcc ctacctcctc acgatggcca aaaaggcttt
    15961 gaaatctgag tccgctgcct caagtacagg gaaggaagat aaacagccag caccagggcc
    16021 tgtggaaaag ccacccagag aacccgcaag gcagctacgg aatcctccaa ccaccattgg
    16081 aatgatgact ctgaaagcag ctttcaagac tctgtctggt gcacaggatt ctgaggcagc
    16141 ctttgcaaaa ctggaccaga aggatctggt tcttcctact caagctctcc cagcatcacc
    16201 agccctcaaa aacaagagac ccagaaaaga tgaaaacgaa agttcagccc cggctgacgg
    16261 tgagggtggc tcggaactgc agcccaagaa caagcgcatg acaataagca gattggtctt
    16321 ggaggaggac agccagagta ctgagcccag cgcaggcctc aactcctccc aggaggccgc
    16381 ttcagcgcca ccatccaagc ccaccgttct caaccaaccc ctccctggag agaagaatcc
    16441 caaagtaccc aaaggcaagt ggaacagctc taatggggtt gaagaaaagg agacttgggt
    16501 ggaagaggat gaactgtttc aagttcaggc accagatgaa gacagtacaa ccaatataac
    16561 aaaaaagcag aagtggactg tagaagaaag cgagtgggtc aaggctggag tgcagaaata
    16621 tggggaagga aactgggctg ccatttctaa aaattaccca tttgttaacc gaacagctgt
    16681 gatgattaag gatcgctggc ggaccatgaa aagacttggc atgaactaat gaaagctgtc
    16741 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
    16801 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    16861 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    16921 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    16981 tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta acaaagcccg
    17041 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac catttaaata
    17101 aatctatatc tcaatcgggg ttacgtcgat accccaccga gaccccattg ggaccaatac
    17161 gcccgcgttt cttccttttc cccaccccaa cccccaagtt cgggtgaagg cccagggctc
    17221 gcagccaacg tcggggcggc aagccctgcc atagccacta cgggttaatt aaaaactata
    17281 tctcaatcgg ggtttaaaca ggagatccga accagataag tgaaatctag ttccaaacta
    17341 ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc catctatttt
    17401 gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta
    17461 ttttgtccgc ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc
    17521 caactccatg tgacaaaccg tcatcttcgg ctacttt
//
