LOCUS       sequence-364978-       11580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV 
            site, and human TRF2 with a zz tag and TEV site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Jack A, Kim Y, Strom AR, Lee DSW, Williams B, Schaub JM, Kellogg EH,
            Finkelstein IJ, Ferro LS, Yildiz A, Brangwynne CP
  TITLE     Compartmentalization of telomeres through DNA-scaffolded phase 
            separation.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 
            10.1016/j.devcel.2021.12.017.
  PUBMED    35077681
REFERENCE   2  (bases 1 to 11580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "277-290.e9"
COMMENT     Sequence Label: pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2
FEATURES             Location/Qualifiers
     source          1..11580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             442..1233
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1274..1728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1361..1380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1570..1591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1754..1858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1859..1927,1928..2719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2077..2096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2890..3478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3379..3398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3632..3649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3783..4007
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4074..4607)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4796..4801,4802..4818,4819..4824))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(5220..5253)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5299..5318)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5343..5391
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5486..5577
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             5633..5650
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5660..6760
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     6734..6757
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             6815..6835
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    8198..8332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        8488..8579
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             8659..8832
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             8833..9006
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             9064..9084
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             9106..9126
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    10884..11018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  11333..11498
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgggaggca gaataaatga tcatatcgtc
      121 aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac
      181 ggtcacactg cttccggtag tcaataaacc ggtaagtagc gtatgcgctc acgcaactgg
      241 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      301 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
      361 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
      421 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
      481 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
      541 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
      601 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
      661 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
      721 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
      781 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
      841 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
      901 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
      961 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     1021 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     1081 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     1141 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     1201 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgg
     1261 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1561 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1621 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1681 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     1741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3001 aactggcttc agcagagcgc agataccaaa tactattctt ctagtgtagc cgtagttagg
     3061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3661 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     3721 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     3781 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     3841 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     3901 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     3961 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     4021 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     4081 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     4141 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     4201 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
     4261 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     4321 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     4381 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     4441 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     4501 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     4561 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca
     4621 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     4681 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     4741 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     4801 acgaaccgaa caggcttatg tcaactgggt ttgtgccttc atccgtttcc acggtgtgcg
     4861 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga
     4921 gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg
     4981 caaggtgctg tgcacggatc tgcccttgct tcaggagatc ggtagacctc ggccgtcgcg
     5041 gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg
     5101 cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc tacagctttg
     5161 tttgtactat caacaggttg aactgctgat caacagatcc tctacgcggc cgcggtacca
     5221 taacttcgta tagcatacat tatacgaagt tatctgggtt taaacgctct atggtctaaa
     5281 gtttttatcg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5341 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     5401 gggttcggtc ccagggctgg cactctgtca agtgaacgct ctatggtcta aagatttaaa
     5461 tcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
     5521 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
     5581 ccggattatt cataccgtcc caccatcggg cgcggatcca atgggttctt ctcaccatca
     5641 ccatcaccat ggttcttcta tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg
     5701 cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat
     5761 taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac
     5821 tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc
     5881 tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac
     5941 ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt
     6001 atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc
     6061 ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga
     6121 accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa
     6181 cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac
     6241 cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
     6301 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
     6361 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
     6421 accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa
     6481 agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt
     6541 taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa
     6601 agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat
     6661 cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg
     6721 tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa
     6781 caacaataac aataacaaca acctcgggat cgaggaaaac ctgtacttcc aatccgactc
     6841 attggagttt atcgcatcaa agctcgcaat ggcggaggcg atggatttgg gcaaagaccc
     6901 caacgggccc acccattcct cgactctgtt cgtgagggac gacggcagct ccatgtcctt
     6961 ctacgtgcgg cccagcccgg ccaagcgtcg gctgtcgacg ctcatcctgc acggcggcgg
     7021 caccgtgtgc cgagtgcagg agcccggggc cgtgctgctg gcccagcccg gggaggcgct
     7081 ggccgaggcc tcgggtgatt tcatctccac gcagtacatc ctggactgcg tggagcgcaa
     7141 cgagaggctg gagctggagg cctatcggct gggccccgcc tcggcggcgg acaccggctc
     7201 ggaagcaaag cccggggccc tggccgaggg cgccgcggag ccggagccgc agcggcacgc
     7261 cgggcggatc gccttcacgg atgcggacga cgtagccatc cttacctacg tgaaggaaaa
     7321 tgcccgctcg cccagctccg tcaccggtaa cgccttgtgg aaagcgatgg agaagagctc
     7381 gctcacgcag cactcgtggc agtccctgaa ggaccgctac ctcaagcacc tgcggggcca
     7441 ggagcataag tacctgctgg gggacgcgcc ggtgagcccc tcctcccaga agctcaagcg
     7501 gaaggcggag gaggacccgg aggccgcgga tagcggggaa ccacagaata agagaactcc
     7561 agatttgcct gaagaagagt atgtgaagga agaaatccag gagaatgaag aagcagtcaa
     7621 aaagatgctt gtggaagcca cccgggagtt tgaggaggtt gtggtggatg agagccctcc
     7681 tgattttgaa atacatataa ctatgtgtga tgatgatcca cccacacctg aggaagactc
     7741 agaaacacag cctgatgagg aggaagaaga agaagaagaa aaagtttctc aaccagaggt
     7801 gggagctgcc attaagatca ttcggcagtt aatggagaag tttaacttgg atctatcaac
     7861 agttacacag gccttcctaa aaaatagtgg tgagctggag gctacttccg ccttcttagc
     7921 gtctggtcag agagctgatg gatatcccat ttggtcccga caagatgaca tagatttgca
     7981 aaaagatgat gaggatacca gagaggcatt ggtcaaaaaa tttggtgctc agaatgtagc
     8041 tcggaggatt gaatttcgaa agaaataaaa gcttgtcgag aagtactaga ggatcataat
     8101 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8161 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     8221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     8341 gcttgagcct agaagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     8401 ccaccgctga gcaataacta tcataaccat ttaaataaac tggatactat tgcacgttta
     8461 aatcgaccta ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg
     8521 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
     8581 ttccggatta ttcataccgt cccaccatcg ggcgcggatc caatgtgcat ggcaggcctt
     8641 gcgcaacacg atgaagccgt ggacaacaaa ttcaacaaag aacaacaaaa cgcgtactat
     8701 gagatcttac atttacctaa cttaaacgaa ggacaacgaa acgccttcat ccaaagttta
     8761 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct
     8821 caggcgccga aagtagacaa caaattcaac aaagaacaac aaaacgcgtt ctatgagatc
     8881 ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat
     8941 gacccaagcc aaagcgctaa ccttttagca gaagctgaaa agctaaatga tgctcaggcg
     9001 ccgaaagtag acgcaaattc cgcggggaag tcaaccgatt atgatattcc aactactgct
     9061 agcgagaacc tatatttcca gggagattat gatatcccta cgactgagaa tctttatttt
     9121 cagggcatgg ccgcgggagc cgggacggcg ggccccgctt ccggcccggg cgtcgtgcgt
     9181 gacccagcgg cgtcacagcc gaggaagcgg cccggccggg agggcgggga gggcgcgcgg
     9241 cgatcggaca cgatggcggg aggaggcggg agtagcgacg gcagcgggcg ggcagctggc
     9301 aggcgggcgt cccgcagtag cgggcgggcc cggcgggggc gccacgagcc ggggctgggg
     9361 ggcccggcgg agcgcggcgc gggggaggca cggctggaag aggcagtcaa tcgctgggtg
     9421 ctcaagttct acttccacga ggcgctgcgg gcctttcggg gtagccggta cggggacttc
     9481 agacagatcc gggacatcat gcaggctttg cttgtcaggc ccttggggaa ggagcacacc
     9541 gtgtcccgat tgctgcgggt tatgcagtgt ctgtcgcgga ttgaagaagg ggaaaattta
     9601 gactgttcct ttgatatgga ggctgagctc acaccactgg aatcagctat caatgtgctg
     9661 gagatgatta aaacggaatt tacactgaca gaagcagtgg tcgaatccag tagaaaactg
     9721 gtcaaggaag ctgctgtcat tatttgtatc aaaaacaaag aatttgaaaa ggcttcaaaa
     9781 attttgaaaa aacatatgtc caaggacccc acaactcaga agctgagaaa tgatctcctg
     9841 aatattattc gagaaaagaa cttggcccat cctgttatcc agaacttttc atatgagacc
     9901 ttccagcaga agatgctgcg cttcctggag agccacctgg atgacgccga gccctacctc
     9961 ctcacgatgg ccaaaaaggc tttgaaatct gagtccgctg cctcaagtac agggaaggaa
    10021 gataaacagc cagcaccagg gcctgtggaa aagccaccca gagaacccgc aaggcagcta
    10081 cggaatcctc caaccaccat tggaatgatg actctgaaag cagctttcaa gactctgtct
    10141 ggtgcacagg attctgaggc agcctttgca aaactggacc agaaggatct ggttcttcct
    10201 actcaagctc tcccagcatc accagccctc aaaaacaaga gacccagaaa agatgaaaac
    10261 gaaagttcag ccccggctga cggtgagggt ggctcggaac tgcagcccaa gaacaagcgc
    10321 atgacaataa gcagattggt cttggaggag gacagccaga gtactgagcc cagcgcaggc
    10381 ctcaactcct cccaggaggc cgcttcagcg ccaccatcca agcccaccgt tctcaaccaa
    10441 cccctccctg gagagaagaa tcccaaagta cccaaaggca agtggaacag ctctaatggg
    10501 gttgaagaaa aggagacttg ggtggaagag gatgaactgt ttcaagttca ggcaccagat
    10561 gaagacagta caaccaatat aacaaaaaag cagaagtgga ctgtagaaga aagcgagtgg
    10621 gtcaaggctg gagtgcagaa atatggggaa ggaaactggg ctgccatttc taaaaattac
    10681 ccatttgtta accgaacagc tgtgatgatt aaggatcgct ggcggaccat gaaaagactt
    10741 ggcatgaact aatgaaagct gtcgagaagt actagaggat cataatcagc cataccacat
    10801 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    10861 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    10921 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10981 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa
    11041 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
    11101 taactatcat aaccatttaa ataaatctat atctcaatcg gggttacgtc gataccccac
    11161 cgagacccca ttgggaccaa tacgcccgcg tttcttcctt ttccccaccc caacccccaa
    11221 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaagccct gccatagcca
    11281 ctacgggtta attaaaaact atatctcaat cggggtttaa acaggagatc cgaaccagat
    11341 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
    11401 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
    11461 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
    11521 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
//
