LOCUS       sequence-364984-       10785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal 
            monomeric EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Jack A, Ferro LS, Trnka MJ, Wehri E, Nadgir A, Nguyenla X, Fox D, 
            Costa K, Stanley S, Schaletzky J, Yildiz A
  TITLE     SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic
            RNA.
  JOURNAL   PLoS Biol. 2021 Oct 11;19(10):e3001425. doi: 
            10.1371/journal.pbio.3001425. eCollection 2021 Oct.
  PUBMED    34634033
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2021-10-11"; volume: "19(10):e3001425. doi"; pages: "
            10.1371/journal.pbio.3001425. eCollection 2021 Oct"
COMMENT     Sequence Label: pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..10785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(9..583,584..586,587..595)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(176..193)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     403..422
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             615..1214
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(615..634)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1111..1131
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1228..1816
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1281..1301)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1699..1710)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2023..2656
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2784..2800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2784..2800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2797..2819)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2808..2824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2832..2838,2839..2856,2857..2862))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2877..2898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3015..3032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3186..3774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3266..3285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3945..4736,4737..4805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4568..4587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4806..4910)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4958..5092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4995..5014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5049..5068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5260..5893
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5940..6065
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6562..6795
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6980..7024
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7287..7402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7596..8778
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7827..8769
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8726..8746
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(10066..10068,10069..10071,10072..10785)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10111..10132)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10372..10391)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10719..10740
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatcccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
       61 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
      121 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
      181 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
      241 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
      301 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
      361 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
      421 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
      481 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
      541 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa
      601 ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      661 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
      721 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
      781 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
      841 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
      901 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
      961 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     1021 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     1081 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     1141 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     1201 agcccggtgc ctagacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     1261 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1321 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     1381 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     1441 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     1501 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     1561 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     1621 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     1681 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     1741 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     1801 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     1861 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     1921 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     1981 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     2041 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     2101 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     2161 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     2221 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     2281 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     2341 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     2401 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     2461 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2521 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2581 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2641 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     2701 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
     2761 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2821 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2881 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2941 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3001 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3061 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3361 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3601 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4921 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
     4981 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5041 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     5101 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     5161 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     5221 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     5281 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     5341 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     5401 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     5461 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     5521 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     5581 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     5641 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     5701 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5761 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5821 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5881 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     5941 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     6001 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     6061 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     6121 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     6181 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     6241 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     6301 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     6361 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     6421 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     6481 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     6541 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     6601 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     6661 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     6721 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     6781 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     6841 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     6901 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     6961 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     7021 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     7081 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     7141 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     7201 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     7261 agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg
     7321 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     7381 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc
     7441 gatgagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt
     7501 aaactcccac taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct
     7561 ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc cggtgcccgt cagtgggcag
     7621 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     7681 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     7741 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     7801 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     7861 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacgcccctg gctgcagtac
     7921 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     7981 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ttgggcgctg gggccgccgc
     8041 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     8101 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     8161 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     8221 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     8281 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     8341 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     8401 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     8461 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     8521 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     8581 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     8641 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     8701 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     8761 ccatttcagg tgtcgtgagg atctatttcc ggtgaattcg ccgccaccat gagcgataac
     8821 ggcccccaaa accaacggaa tgcccctcgg ataacatttg gtggccctag tgattccaca
     8881 ggaagtaacc aaaacggaga acggtctgga gcccgctcta aacagaggcg accacaggga
     8941 ttgccaaata acacagcgtc atggtttaca gccctgactc aacatggaaa ggaggacttg
     9001 aaatttccac gaggacaggg tgtaccaata aacacaaaca gtagtccaga cgatcagatc
     9061 gggtattata gaagggccac gagacgcatc agaggtggag atggtaaaat gaaagatctc
     9121 agtccgcgct ggtactttta ttatttggga accggcccag aagcgggcct cccatatgga
     9181 gccaataagg acgggattat atgggtggct acagagggag ccctcaatac gccaaaagat
     9241 cacatcggaa caagaaaccc tgctaataat gccgcaatcg tgctgcagtt gcctcagggg
     9301 acgacgcttc ctaaaggctt ttacgcagaa ggatcacgcg gcggcagcca agcatcctca
     9361 aggtctagtt ccagaagtcg aaacagctcc agaaactcca caccagggtc cagtaggggc
     9421 acaagtccgg cgcggatggc gggcaacggc ggagacgccg cactcgctct gttgctcctg
     9481 gaccggctca accaacttga atccaagatg agtggtaagg gacagcagca acaaggtcaa
     9541 accgtaacca agaaaagcgc tgcagaagct tctaaaaaac ctcgacaaaa acggaccgct
     9601 acgaaggcat ataacgttac tcaagccttc gggagacgag ggccggagca aacccagggg
     9661 aattttggag atcaggagct catccggcaa gggacagatt acaaacattg gcctcaaatc
     9721 gctcagttcg cgccgtccgc aagtgctttt ttcggaatgt ctcgcatcgg tatggaagta
     9781 acaccatccg ggacatggct cacttatacc ggcgccatca agctggacga caaagatcca
     9841 aatttcaaag atcaggtgat tctcttgaat aaacacatag acgcgtataa gacgtttcca
     9901 cccaccgaac ccaagaagga caaaaagaaa aaagctgatg aaacacaagc gttgccccag
     9961 cgacagaaga agcagcaaac cgtcaccctc ctgccagccg ctgatctcga tgacttctcc
    10021 aagcaacttc aacagtccat gagctcagcc gattctactc aggcgatggt gagcaagggc
    10081 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
    10141 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
    10201 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
    10261 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
    10321 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
    10381 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
    10441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
    10501 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
    10561 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
    10621 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
    10681 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
    10741 accgccgccg ggatcactct cggcatggac gagctgtaca agtaa
//
