LOCUS       sequence-364994-        9738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus expression for SLC31A1 gene  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, 
            Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel 
            E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub 
            TR
  TITLE     Copper induces cell death by targeting lipoylated TCA cycle 
            proteins.
  JOURNAL   Science. 2022 Mar 18;375(6586):1254-1261. doi: 
            10.1126/science.abf0529. Epub 2022 Mar 17.
  PUBMED    35298263
REFERENCE   2  (bases 1 to 9738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf0529"; journalName: "Science"; date: 
            "2022-03-18- 18"; volume: "375"; issue: "6586"; pages: "1254-1261"
COMMENT     Sequence Label: PLX_307 SLC31A1
FEATURES             Location/Qualifiers
     source          1..9738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        197..407
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        219..415
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      266..401
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             447..1049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     943..963
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1092..1209
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1347..2525
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1578..2516
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2473..2493
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2545..2569
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3140..3164)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3172..3213
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3324..3912
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3377..3397)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3795..3806)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3984..4217
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4295..4416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4332..4351)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4386..4405
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4456..4591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4611..4630)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4612..4630)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4640..4657)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4640..4656)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4649..4671)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4798..5253
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4885..4904)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5095..5116
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5279..5383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5384..5452,5453..6244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5602..5621)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6415..7003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6904..6923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7157..7174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7291..7312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7327..7332,7333..7350,7351..7357)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7365..7381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7370..7392
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7389..7405
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7389..7405
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7424..7444
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7426..7444
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7472..7698
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7699..7879
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7926..8051
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8544..8777
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8962..9006
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9237..9447
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9259..9455
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9306..9441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
       61 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
      121 tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa
      181 gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac
      241 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      301 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      361 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
      421 ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
      481 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
      541 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
      601 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
      661 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
      721 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
      781 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
      841 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
      901 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
      961 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     1021 gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta
     1081 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1141 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1201 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc
     1261 tgcaggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     1321 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     1381 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     1441 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     1501 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     1561 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     1621 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     1681 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     1741 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     1801 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     1861 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     1921 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     1981 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     2041 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     2101 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     2161 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     2221 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     2281 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     2341 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     2401 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     2461 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     2521 cgtgaggcta gcatcgattg atcaacaagt ttgtacaaaa aagttggcat ggatcattcc
     2581 caccatatgg ggatgagcta tatggactcc aacagtacca tgcaaccttc tcaccatcac
     2641 ccaaccactt cagcctcaca ctcccatggt ggaggagaca gcagcatgat gatgatgcct
     2701 atgaccttct actttggctt taagaatgtg gaactactgt tttccggttt ggtgatcaat
     2761 acagctggag aaatggctgg agcttttgtg gcagtgtttt tactagcaat gttctatgaa
     2821 ggactcaaga tagcccgaga gagcctgctg cgtaagtcac aagtcagcat tcgctacaat
     2881 tccatgcctg tcccaggacc aaatggaacc atccttatgg agacacacaa aactgttggg
     2941 caacagatgc tgagctttcc tcacctcctg caaacagtgc tgcacatcat ccaggtggtc
     3001 ataagctact tcctcatgct catcttcatg acctacaacg ggtacctctg cattgcagta
     3061 gcagcagggg ccggtacagg atacttcctc ttcagctgga agaaggcagt ggtagtggat
     3121 atcacagagc attgccattg cccaactttc ttgtacaaag tggttgatat cggtaagcct
     3181 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     3241 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacagact ttctcataga
     3301 ttaggaatac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     3361 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3421 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3481 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     3541 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3601 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3661 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3721 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3781 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3841 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3901 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     3961 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     4021 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     4081 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4141 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4201 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     4261 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     4321 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     4441 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4501 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4561 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     4621 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     4681 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     4741 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     4801 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4861 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4921 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4981 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5041 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5101 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5161 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5221 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     5281 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5341 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5401 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5461 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5521 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5581 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5641 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5701 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5761 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5821 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5881 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5941 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6001 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6061 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6121 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6181 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6241 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6301 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6541 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7081 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7141 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7201 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7261 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7321 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7381 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     7441 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     7501 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     7561 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     7621 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     7681 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7741 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7801 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7861 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     7921 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     7981 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     8041 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     8101 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     8161 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     8221 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     8281 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     8341 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     8401 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     8461 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     8521 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     8581 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     8641 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8701 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8761 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8821 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     8881 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     8941 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     9001 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     9061 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     9121 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     9181 atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9241 gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc
     9301 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9361 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     9421 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag
     9481 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     9541 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     9601 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     9661 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg
     9721 ccggagagcg tcgaagcg
//
