LOCUS       sequence-364998-        9402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus expression for SLC31A1 gene  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, 
            Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel 
            E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub 
            TR
  TITLE     Copper induces cell death by targeting lipoylated TCA cycle 
            proteins.
  JOURNAL   Science. 2022 Mar 18;375(6586):1254-1261. doi: 
            10.1126/science.abf0529. Epub 2022 Mar 17.
  PUBMED    35298263
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf0529"; journalName: "Science"; date: 
            "2022-03-18- 18"; volume: "375"; issue: "6586"; pages: "1254-1261"
COMMENT     Sequence Label: PLEX_313 SLC31A1
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     391..408
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    525..546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(561..566,567..584,585..591)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    599..615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     604..626
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     623..639
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..639
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     658..678
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        660..678
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        706..932
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             933..1113
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1160..1285
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1778..2011
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2196..2240
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2471..2681
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2493..2689
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2540..2675
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2711..3730
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    3801..3918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4056..5234
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4287..5225
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5182..5202
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5254..5278
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5849..5873)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5881..5922
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5973..6561
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6026..6046)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6444..6455)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6633..6866
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6944..7065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6981..7000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7035..7054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7105..7240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7260..7279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7261..7279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7289..7306)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7289..7305)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7298..7320)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7447..7902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7534..7553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7744..7765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7928..8032
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8033..8101,8102..8893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8251..8270)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9064..9402,1..237)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgatgatgc tcgtcagggg ggcggagcct atggaaaaac
      241 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      301 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      361 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      421 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      481 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      541 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      601 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
      661 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
      721 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      781 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      841 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      901 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      961 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     1021 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     1081 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     1141 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     1201 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     1261 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     1321 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     1381 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     1441 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1501 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1561 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1621 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1681 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1741 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1801 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1861 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1921 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1981 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     2041 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     2101 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     2161 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     2221 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     2281 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     2341 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     2401 ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     2461 aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt
     2521 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     2581 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     2641 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag
     2701 aggtaccacc atgaaaaagc ctgaactcac cgctacctct gtcgagaagt ttctgatcga
     2761 aaagttcgac agcgtctccg acctgatgca gctctccgag ggcgaagaat ctcgggcttt
     2821 cagcttcgat gtgggagggc gtggatatgt cctgcgggtg aatagctgcg ccgatggttt
     2881 ctacaaagat cgctatgttt atcggcactt tgcatccgcc gctctcccta ttcccgaagt
     2941 gcttgacatt ggggagttca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     3001 tgtcaccttg caagacctgc ctgaaaccga actgcccgct gttctccagc ccgtcgccga
     3061 ggccatggat gccatcgctg ccgccgatct tagccagacc agcgggttcg gcccattcgg
     3121 acctcaagga atcggtcaat acactacatg gcgcgatttc atctgcgcta ttgctgatcc
     3181 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcctccg tcgcccaggc
     3241 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     3301 cgatttcggc tccaacaatg tcctgaccga caatggccgc ataacagccg tcattgactg
     3361 gagcgaggcc atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     3421 ctggttggct tgtatggagc agcagacccg ctacttcgag cggaggcatc ccgagcttgc
     3481 aggatctcct cggctccggg cttatatgct ccgcattggt cttgaccaac tctatcagag
     3541 cttggttgac ggcaatttcg atgatgcagc ttgggctcag ggtcgctgcg acgcaatcgt
     3601 ccggtccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg ctgccgtctg
     3661 gaccgatggc tgtgtggaag tgctcgccga tagtggaaac agacgcccca gcactcgtcc
     3721 tagggcaaag gatctgcagt aatgaggcgc gccgttaacg aattctagat cttgagacaa
     3781 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     3841 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     3901 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     3961 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     4021 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     4081 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     4141 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     4201 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     4261 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     4321 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     4381 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     4441 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     4501 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     4561 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     4621 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     4681 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     4741 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     4801 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     4861 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     4921 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     4981 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     5041 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     5101 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     5161 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     5221 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agttggcatg
     5281 gatcattccc accatatggg gatgagctat atggactcca acagtaccat gcaaccttct
     5341 caccatcacc caaccacttc agcctcacac tcccatggtg gaggagacag cagcatgatg
     5401 atgatgccta tgaccttcta ctttggcttt aagaatgtgg aactactgtt ttccggtttg
     5461 gtgatcaata cagctggaga aatggctgga gcttttgtgg cagtgttttt actagcaatg
     5521 ttctatgaag gactcaagat agcccgagag agcctgctgc gtaagtcaca agtcagcatt
     5581 cgctacaatt ccatgcctgt cccaggacca aatggaacca tccttatgga gacacacaaa
     5641 actgttgggc aacagatgct gagctttcct cacctcctgc aaacagtgct gcacatcatc
     5701 caggtggtca taagctactt cctcatgctc atcttcatga cctacaacgg gtacctctgc
     5761 attgcagtag cagcaggggc cggtacagga tacttcctct tcagctggaa gaaggcagtg
     5821 gtagtggata tcacagagca ttgccattgc ccaactttct tgtacaaagt ggttgatatc
     5881 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtacc
     5941 ggttaagtcg acaatcaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     6001 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6061 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6121 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6181 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6241 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6301 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6361 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6421 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6481 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6541 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     6601 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6661 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6721 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6781 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6841 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     6901 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     6961 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7021 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7081 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7141 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7201 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     7261 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     7321 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7381 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7441 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7501 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7561 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7621 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7681 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7741 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7801 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7861 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     7921 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7981 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8041 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8101 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8161 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8221 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8281 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8341 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8401 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8461 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8521 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8581 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8641 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8701 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8761 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8821 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8881 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8941 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9001 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9061 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9121 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9181 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9241 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9301 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9361 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca ca
//
