LOCUS       sequence-364999-        9601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus expression for GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, 
            Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel 
            E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub 
            TR
  TITLE     Copper induces cell death by targeting lipoylated TCA cycle 
            proteins.
  JOURNAL   Science. 2022 Mar 18;375(6586):1254-1261. doi: 
            10.1126/science.abf0529. Epub 2022 Mar 17.
  PUBMED    35298263
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf0529"; journalName: "Science"; date: 
            "2022-03-18- 18"; volume: "375"; issue: "6586"; pages: "1254-1261"
COMMENT     Sequence Label: PLEX_313 GFP
FEATURES             Location/Qualifiers
     source          1..9601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    54..175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(65..84)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     119..138
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             253..486
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    558..1146
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             664..675
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1073..1093
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1197..1238)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1246..1270
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1290..2000,2001..2003,2004..2006))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1332..1353)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1681..1700
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1940..1961
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(2027..2051)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2071..3249)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2080..3018
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(2103..2123)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3387..3504
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3575..4594)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     promoter        complement(4616..4812)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(4624..4834)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4630..4765
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(5065..5109)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5294..5527
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6020..6145
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6192..6372
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6373..6599)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6627..6647)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6627..6645)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6666..6682)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6666..6682)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6679..6701)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6690..6706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6714..6720,6721..6738,6739..6744))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6759..6780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6897..6914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7068..7656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7148..7167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7827..8618,8619..8687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8450..8469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8688..8792)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8818..9273)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8955..8976)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9167..9186
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9400..9422
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9414..9431
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9415..9431
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9441..9460
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9441..9459
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      join(9480..9601,1..14)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gttaggggcg ggatgggcgg agttaggggc gggatagcta gagccagaca tgataagata
       61 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
      121 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttcctct
      181 cactctctga tattcatttc tttgcaagtt ataaatactg aataataaga tgacatgaac
      241 tactatacgt actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
      301 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
      361 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
      421 accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgaattagcc
      481 cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
      541 ttggtcttaa agtcgacgcg gggaggcggc ccaaagggag atccgactcg tctgagggcg
      601 aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc
      661 tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac
      721 acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt
      781 gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt
      841 ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc
      901 ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac
      961 gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa
     1021 agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt
     1081 aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt aatccagagg
     1141 ttgattgtcg acttaacgcg ttgattgtcg acttaaccgg tactagttca ttactacgta
     1201 gaatcgagac cgaggagagg gttagggata ggcttaccga tatcaaccac tttgtacaag
     1261 aaagctgggt cggcgcgccc acccttggac ttgtacagct cgtccatgcc gagagtgatc
     1321 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     1381 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     1441 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
     1501 cggatcttga agttcgcctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
     1561 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
     1621 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
     1681 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
     1741 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
     1801 acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     1861 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccagacacg
     1921 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     1981 aacagctcct cgcccttgct caccatggtg aagggggcgg ccgcggagcc tgcttttttg
     2041 tacaaacttg ttgatcaatc gatgctagcc tcacgacacc tgaaatggaa gaaaaaaact
     2101 ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc
     2161 caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc actcagtgtg
     2221 gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac tccaaaagct
     2281 cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac atgaagcgac
     2341 ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc gctctcccga
     2401 gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc atctttccgc
     2461 tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga tacacggcgg
     2521 cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc cgattctcgg
     2581 tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac gggccccgtc
     2641 gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg catttacaag
     2701 actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga
     2761 cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag attcgcacgc ggcggcccca
     2821 gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct
     2881 cgaactctcc cacccacttc caacccgaag ctcgggatca agaatcacgt actgcagcca
     2941 ggtggaagta attcaaggca cgcaagggcc ataacccgta aagaggccag gcccgcggga
     3001 accacacacg gcacttacct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac
     3061 ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta
     3121 cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc
     3181 gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc
     3241 accggagcca attcccactc ctttcaagac ctagaaggtc cattagctgc aaagattcct
     3301 ctctgtttaa aactttatcc atctttgcac ctgcaggcct cgagccggcg ccaaagtgga
     3361 tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat
     3421 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
     3481 ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct caagatctag
     3541 aattcgttaa cggcgcgcct cattactgca gatcctttgc cctaggacga gtgctggggc
     3601 gtctgtttcc actatcggcg agcacttcca cacagccatc ggtccagacg gcagcgcttc
     3661 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga ccggacgatt gcgtcgcagc
     3721 gaccctgagc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     3781 caagaccaat gcggagcata taagcccgga gccgaggaga tcctgcaagc tcgggatgcc
     3841 tccgctcgaa gtagcgggtc tgctgctcca tacaagccaa ccagggcctc cagaagaaga
     3901 tgttggcgac ctcgtattgg gaatccccga acatggcctc gctccagtca atgacggctg
     3961 ttatgcggcc attgtcggtc aggacattgt tggagccgaa atcggcgtgc acgaggtgcc
     4021 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgg gcgacggagg
     4081 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatagcgc
     4141 agatgaaatc gcgccatgta gtgtattgac cgattccttg aggtccgaat gggccgaacc
     4201 cgctggtctg gctaagatcg gcggcagcga tggcatccat ggcctcggcg acgggctgga
     4261 gaacagcggg cagttcggtt tcaggcaggt cttgcaaggt gacaccctgt gcacggcggg
     4321 agatgcaata ggtcaggctc tcgctgaact ccccaatgtc aagcacttcg ggaataggga
     4381 gagcggcgga tgcaaagtgc cgataaacat agcgatcttt gtagaaacca tcggcgcagc
     4441 tattcacccg caggacatat ccacgccctc ccacatcgaa gctgaaagcc cgagattctt
     4501 cgccctcgga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     4561 cgacagaggt agcggtgagt tcaggctttt tcatggtggt acctctagaa agctttttgc
     4621 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     4681 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     4741 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
     4801 aattgagacg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     4861 tggttgctga ctaattgaga tgcaatgcat gggtcccctc ggggttggga ggtgggtctg
     4921 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
     4981 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
     5041 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     5101 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     5161 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     5221 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     5281 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     5341 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     5401 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     5461 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     5521 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     5581 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
     5641 gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct
     5701 tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta
     5761 tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg
     5821 atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt
     5881 ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt
     5941 ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa
     6001 ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag
     6061 tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag
     6121 caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt
     6181 tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     6241 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     6301 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     6361 ccagagagac ccgtttatgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg
     6421 caattcagtg gttcgtccaa tccatgtcag acccgtctgt tgccttccta ataaggcacg
     6481 atcgtaccac cttacttcca ccaatcggca tgcacggtgc tttttctctc cttgtaaggc
     6541 atgttgctaa ctcatcgtta ccatgttgca agactacaag agtattgcat aagactacat
     6601 taagcttgca gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     6661 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6721 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6781 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6841 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6961 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7021 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7081 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7141 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7201 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7261 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7321 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7381 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7441 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7501 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7561 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7621 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7681 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7741 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7801 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7861 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7921 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7981 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8041 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8101 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8161 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8221 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8281 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8341 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8401 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8461 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8521 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8581 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8641 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     8821 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     8881 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     8941 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     9001 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     9061 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     9121 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     9181 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     9241 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     9301 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     9361 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     9421 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt acgtccctag
     9481 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     9541 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     9601 a
//
