LOCUS       sequence-36500-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..48
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     58..82
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             164..187
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             200..226
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    242..266
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             290..310
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttagcgactc tcctatagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
       61 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      121 aagacaccgg gaccgatcca gcctccgggg atctgccacc atggactaca aggatgacga
      181 tgacaagctc gatggaggat acccatacga tgttccagat tacgctgctc gaggttatca
      241 aacaagtttg tacaaaaaag caggctccgc ggccgccccc ttcacccctc ctaagaagaa
      301 gaggaaggtt cggcaccaca agtcggaccc ctactcaacc ggtcacctcc gcgaagcccc
      361 ctccccgctt atgatacatt ccccccagat gtcacagttc cagagcctga cccaccagtc
      421 tccaccccag caaaacgtcc agcctaagaa acaggagctg cgtgctgcct ccgtggtcca
      481 gccccagccc ctcgtggtgg tgaaggagga gaagatccac tcacccatca tccgcagcga
      541 gcccttcagc ccctcgctgc ggccggagcc ccccaagcac ccggagagca tcaaggcccc
      601 cgtccacctg ccccagcggc cggaaatgaa gcctgtggat gtcgggaggc ctgtgatccg
      661 gcccccagag cagaacgcac cgccaccagg ggcccctgac aaggacaaac agaaacagga
      721 gccgaagact ccagttgcgc ccaaaaagga cctgaaaatc aagaacatgg gctcctgggc
      781 cagcctagtg cagaagcatc cgaccacccc ctcctccaca gccaagtcat ccagcgacag
      841 cttcgagcag ttccgccgcg ccgctcggga gaaagaggag cgtgagaagg ccctgaaggc
      901 tcaggccgag cacgctg
//
