LOCUS       sequence-36503-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6350 MSCV-CMV-CMV-Flag-HA-ATAD5
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             165..188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             201..227
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    243..267
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      301..310
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tagcgactct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccggg gatctgccac catggactac aaggatgacg
      181 atgacaagct cgatggagga tacccatacg atgttccaga ttacgctgct cgaggttatc
      241 aaacaagttt gtacaaaaaa gcagaagggc cgtcaaggcc caccatggtg ggggtcctgg
      301 ccatggcggc tgcagctgct ccgcctcccg tgaaggactg cgagattgag ccatgcaaaa
      361 agcgaaagaa agatgatgac acatctacct gcaaaacaat tacaaaatat ttatcaccac
      421 tagggaagac tagagacagg gtttttgctc caccaaaacc tagtaatatt ctggattatt
      481 ttagaaagac ttcacccaca aatgagaaga cacaattagg gaaagagtgc aagataaagt
      541 cacctgaatc agtacctgtt gacagcaaca aagactgtac gacacctttg gaaatgttct
      601 caaatgtaga gtttaagaag aaaagaaaga gggttaattt atctcatcaa ctaaataata
      661 ttaaaactga aaatgaagct ccaattgaaa ttagtagcga cgatagcaaa gaagactata
      721 gtttaaataa tga
//
