LOCUS       sequence-36504-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6351 MSCV-CMV-CMV-Flag-HA-NSD3
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..56
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     66..90
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      163..172
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             172..195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             208..234
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    250..274
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggctttag cgagctctcc tatcagtgat agagatctcc ctatcagtga tagagatcgt
       61 cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
      121 ctccatagaa gacaccggga ccgatccagc ctccggggat ctgccaccat ggactacaag
      181 gatgacgatg acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga
      241 ggttatcaaa caagtttgta caaaaaagtt ggcatggatt tctctttctc tttcatgcaa
      301 gggatcatgg gaaacacaat tcagcaacca cctcaactca ttgactccgc caacatccgt
      361 caggaggatg cctttgataa caacagtgac attgctgaag atggtggcca gacaccatat
      421 gaagctactt tgcagcaagg ctttcagtac ccagctacaa cagaagatct tcctccactc
      481 acaaatgggt atccatcatc aatcagtgtg tatgaaactc aaaccaaata ccagtcatat
      541 aatcagtatc ctaatgggtc agccaatggc tttggtgcag ttagaaactt tagccccact
      601 gactattatc attcagaaat tccaaacaca agaccacatg aaattctgga aaaaccttcc
      661 cctccacagc caccacctcc tccttcggta ccacaaactg tgattccaaa gaagactggc
      721 tcacctgaaa ttaaactaaa aataaccaaa act
//
