LOCUS       sequence-365052-        7630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct to ubiquitously express mouse Cyp26a1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Amamoto R, Wallick GK, Cepko CL
  TITLE     Retinoic acid signaling mediates peripheral cone photoreceptor 
            survival in a mouse model of retina degeneration.
  JOURNAL   Elife. 2022 Mar 22;11. pii: 76389. doi: 10.7554/eLife.76389.
  PUBMED    35315776
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-22"; volume: "11. pii: 76389. doi"; pages: " 
            10.7554/eLife.76389"
COMMENT     Sequence Label: AAV-CMV-Cyp26a1
FEATURES             Location/Qualifiers
     source          1..7630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     primer_bind     complement(192..211)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        313..692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        693..896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     847..867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     893..917
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1010..1485
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(3054..3070)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3055..3071)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3164..3558
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     repeat_region   3913..4042
                     /label=AAV2 ITR
     rep_origin      4413..4926
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4500..4519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4710..4731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5408..5427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5527..5549
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5587..5605)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5673..5777
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5778..5846,5847..6638)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5996..6015)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6809..7397
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7298..7317
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 akgcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttggcc
      241 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
      301 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      361 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      421 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      481 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      541 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      601 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      661 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      721 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      781 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      841 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
      901 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      961 accgggaccg atccagcctc ccctcgaagc tgatcctgag aacttcaggg tgagtctatg
     1021 ggacccttga tgttttcttt ccccttcttt tctatggtta agttcatgtc ataggaaggg
     1081 gagaagtaac agggtacaca tattgaccaa atcagggtaa ttttgcattt gtaattttaa
     1141 aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata ctttccctaa
     1201 tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac cattctaaag
     1261 aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat aaatatttct
     1321 gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct acaatccagc
     1381 taccattctg cttttatttt atggttggga taaggctgga ttattctgag tccaagctag
     1441 gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct gggcaacgtg
     1501 ctggtctgtg tgctggccca tcactttggc aaagaattcc gcgggcccgg gatccaatgg
     1561 ggctcccggc gctgctggcc agtgcgctct gcaccttcgt gctgccgctg ctgctcttcc
     1621 tggcggcgct caagctctgg gacctgtact gtgtgagcag ccgcgatcgc agctgcgccc
     1681 tccccttgcc ccccggtacc atgggcttcc cattctttgg ggaaacattg cagatggtgc
     1741 ttcagcggag gaagtttctg cagatgaagc gcaggaaata cggcttcatc tacaagacgc
     1801 atctgtttgg gcggcccacg gtgcgggtga tgggcgcgga taatgtgcgg cgcatcttgc
     1861 tgggagagca ccggttggtg tcggtgcact ggcccgcgtc ggtgcgcacc atcctgggcg
     1921 ctggctgcct ctccaacctg cacgattcct cgcacaagca gcgaaagaag gtgattatgc
     1981 aggccttcag ccgcgaggca ctccagtgct acgtgcccgt gatcgctgag gaagtcagca
     2041 gttgtctgga gcagtggcta agctgcggcg agcgcggcct cctggtctac cccgaggtga
     2101 agcgcctcat gttccgcatc gccatgcgca tcctgctggg ctgcgagccg ggtccagcgg
     2161 gcggcgggga ggacgagcag cagctcgtgg aggctttcga ggagatgacc cgcaatctct
     2221 tctctcttcc cattgacgtg ccctttagcg gcctgtaccg gggcgtgaag gcgcggaacc
     2281 ttatacacgc gcgcatcgag gagaacattc gcgccaagat ccgccggctt caggctacag
     2341 agccggatgg gggttgcaag gacgcgctgc agctcctgat tgagcactcg tgggagaggg
     2401 gagagaggct ggatatgcag gcactaaaac aatcgtcaac agagctcctc tttggtggtc
     2461 atgaaactac agccagtgct gcgacgtcac tgatcactta cctaggactc tacccacatg
     2521 tcctccagaa agttcgagaa gagataaaga gcaagggctt actttgcaag agcaatcaag
     2581 acaacaagtt agacatggaa actttggaac agcttaaata cactgggtgt gtcattaagg
     2641 agaccctgcg attgaatcct ccggttccag gagggtttcg ggttgctctg aagacttttg
     2701 agctgaatgg ataccagatc cccaagggct ggaatgttat ttacagtatc tgtgacaccc
     2761 acgatgtggc agatatcttc actaacaagg aggaatttaa tcccgaccgc tttatagtgc
     2821 ctcatccaga ggatgcttcc cggttcagct tcattccatt tggaggaggc cttcggagct
     2881 gtgtaggcaa agagtttgca aaaattcttc ttaagatatt tacagtggag ctggctaggc
     2941 actgtgattg gcagcttcta aatggacctc ctacaatgaa gacaagcccc actgtgtacc
     3001 ctgtggacaa tctccctgca agatttaccc acttccaggg agatatctga tatcgatacc
     3061 gtcgacctcg agggcccaga tctaattcac cccaccagtg caggctgcct atcagaaagt
     3121 ggtggctggt gtggctaatg ccctggccca caagtatcac taagctcgct ttcttgctgt
     3181 ccaatttcta ttaaaggttc ctttgttccc taagtccaac tactaaactg ggggatatta
     3241 tgaagggcct tgagcatctg gattctgcct aataaaaaac atttattttc attgcaatga
     3301 tgtatttaaa ttatttctga atattttact aaaaagggaa tgtgggaggt cagtgcattt
     3361 aaaacataaa gaaatgaaga gctagttcaa accttgggaa aatacactat atcttaaact
     3421 ccatgaaaga aggtgaggct gcaaacagct aatgcacatt ggcaacagcc cctgatgcct
     3481 atgccttatt catccctcag aaaaggattc aagtagaggc ttgatttgga ggttaaagtt
     3541 ttgctatgct gtattttaca ttacttattg ttttagctgt cctcatgaat gtcttttcac
     3601 tacccatttg cttatcctgc atctctcagc cttgactcca ctcagttctc ttgcttagag
     3661 ataccacctt tcccctgaag tgttccttcc atgttttacg gcgagatggt ttctcctcgc
     3721 ctggccactc agccttagtt gtctctgttg tcttatagag gtctacttga agaaggaaaa
     3781 acagggggca tggtttgact gtcctgtgag cccttcttcc ctgcctcccc cactcacagt
     3841 gacccggaat ccctcgacat ctagagcatg gctacgtaga taagtagcat ggcgggttaa
     3901 tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     3961 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4021 cagtgagcga gcgagcgcgc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4081 cccaacagtt gcgcagcctg aatggcgaat ggcgattccg ttgcaatggc tggcggtaat
     4141 attgttctgg atattaccag caaggccgat agtttgagtt cttctactca ggcaagtgat
     4201 gttattacta atcaaagaag tattgcgaca acggttaatt tgcgtgatgg acagactctt
     4261 ttactcggtg gcctcactga ttataaaaac acttctcagg attctggcgt accgttcctg
     4321 tctaaaatcc ctttaatcgg cctcctgttt agctcccgct ctgattctaa cgaggaaagc
     4381 acgttatacg tgctcgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4441 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4501 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4561 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4621 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4681 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4741 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4801 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     4861 tacaatttaa atatttgctt atacaatctt cctgtttttg gggcttttct gattatcaac
     4921 cggggtacat atgattgaca tgctagtttt acgattaccg ttcatcgatt ctcttgtttg
     4981 ctccagactc tcaggcaatg acctgatagc ctttgtagag acctctcaaa aatagctacc
     5041 ctctccggca tgaatttatc agctagaacg gttgaatatc atattgatgg tgatttgact
     5101 gtctccggcc tttctcaccc gtttgaatct ttacctacac attactcagg cattgcattt
     5161 aaaatatatg agggttctaa aaatttttat ccttgcgttg aaataaaggc ttctcccgca
     5221 aaagtattac agggtcataa tgtttttggt acaaccgatt tagctttatg ctctgaggct
     5281 ttattgctta attttgctaa ttctttgcct tgcctgtatg atttattgga tgttggaatc
     5341 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     5401 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5461 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5521 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5581 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5641 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5701 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5761 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5821 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5881 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5941 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6001 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6061 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6121 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6181 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6241 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6301 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6361 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6421 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6481 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6541 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6601 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6661 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6721 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6781 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6841 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6901 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6961 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7021 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7081 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7141 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7201 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7261 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7321 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7381 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7441 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7501 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7561 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7621 ttcattaatg
//
