LOCUS       sequence-36511-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Wirsing A, Johnstone KA, Harries LW, Ellard S, Ryffel GU, Stanik J, 
            Gasperikova D, Klimes I, Murphy R
  TITLE     Novel monogenic diabetes mutations in the P2 promoter of the HNF4A 
            gene are associated with impaired function in vitro.
  JOURNAL   Diabet Med. 2010 Jun . 27(6):631-5.
  PUBMED    20546279
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Diabet Med.
            2010 Jun . 27(6):631-5."
COMMENT     Sequence Label: HNF4A_P2-285/-169
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    408..499
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(440..459)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    513..561
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(829..850)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggcgccggg cctttcttta tgtttttggc gtcttccatg gtggctttac caacagtacc
       61 ggaatgccaa gcttgggcca agcccaccca gccggagagc tgggggcatg gaggagcagg
      121 ggcccgctca cagcagcagc acagccacca ggagcccgtc caccaggaag gcggtgagtg
      181 cggccagctg ccaccgcccg gttatcttat tgattcttct aatcacccaa ggtgggtgga
      241 tacattaaag agtaaccagt cacttaggga acccgcggct ggggactgtt gctgtctcca
      301 tggcaatggc gacctggcca gacctgaggt ccactcgggt cctagtcctg cactctgggg
      361 ccgctcgagc ccgggctagc acgcgtaaga gctcggtacc tatcgataga gaaatgttct
      421 ggcacctgca cttgcactgg ggacagccta ttttgctagt ttgttttgtt tcgttttgtt
      481 ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac acagatgtaa
      541 tgaaaataaa gatattttat tgcggccgct ccaagtacct cccgtacctt aatattactt
      601 acttatcatg gtagcttggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      661 caacagttgc gcagcctgaa tggcgaatgg caaattgtaa gcgttaatat tttgttaaaa
      721 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
      781 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
      841 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatc
//
