LOCUS       sequence-36512-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A
  TITLE     Biochemical analysis of the canonical model for the mammalian 
            circadian clock.
  JOURNAL   J Biol Chem. 2011 Jul 22;286(29):25891-902. doi: 
            10.1074/jbc.M111.254680. Epub 2011 May 25.
  PUBMED    21613214
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.254680"; journalName: "J Biol Chem"; date: 
            "2011-07-22- 22"; volume: "286"; issue: "29"; pages: "25891-902"
COMMENT     Sequence Label: pCKPC4
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             121..138
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             139..162
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagttaag cttggtaccg agctcggatc caccatggac
      121 catcaccatc accatcacga ctacaaagac gatgacgaca agatggtgtt taccgtaagc
      181 tgtagtaaaa tgagctcaat tgttgacaga gatgacagta gtatttttga tggattggtg
      241 gaagaagatg acaaggacaa agcaaaaaga gtatctagaa acaaatcaga aaagaaacgt
      301 agagatcagt tcaatgtcct cattaaggag ctggggtcta tgcttcctgg taacgcgaga
      361 aagatggaca agtctactgt tctacagaag agcattgatt ttttgcgcaa acataaagag
      421 accactgcac agtcagatgc tagtgagatt cgacaggact ggaaacccac attccttagt
      481 aatgaagagt ttacacagtt aatgttagag gctcttgatg gttttttttt agcgatcatg
      541 acagatggaa gtataatata tgtatctgag agtgtaactt cgttacttga acatttacca
      601 tctgatcttg tggatcaaag tatatttaat tttatcccag agggagaaca ttcagaggtt
      661 tataagatac tctctactcc tcctgctgga aagtgactca tta
//
