LOCUS       sequence-36514-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Wirsing A, Johnstone KA, Harries LW, Ellard S, Ryffel GU, Stanik J, 
            Gasperikova D, Klimes I, Murphy R
  TITLE     Novel monogenic diabetes mutations in the P2 promoter of the HNF4A 
            gene are associated with impaired function in vitro.
  JOURNAL   Diabet Med. 2010 Jun . 27(6):631-5.
  PUBMED    20546279
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Diabet Med.
            2010 Jun . 27(6):631-5."
COMMENT     Sequence Label: HNF4A_P2-285/-192
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    410..501
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(442..461)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    515..563
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(831..852)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta
       61 ccggaatgcc aagcttgggc caagcccacc cagccggaga gctgggggca tggaggagca
      121 ggggcccgct cacagcagca gcacagccac caggagcccg tccaccagga aggcggtgag
      181 tgcggccagc tgccaccgcc cggttatctt attgattctt ctaatcaccc aaggtgggtg
      241 gatacgttaa agagtaacca gtcacttacg gaacccgcgg ctggggactg ttgctgtctc
      301 catggcaatg gcgacctggc cagacctgag gtccactcgg gtcctagtcc tgcactctgg
      361 ggccgctcga gcccgggcta gcacgcgtaa gagctcggta cctatcgata gagaaatgtt
      421 ctggcacctg cacttgcact ggggacagcc tattttgcta gtttgttttg tttcgttttg
      481 ttttgatgga gagcgtatgt tagtactatc gattcacaca aaaaaccaac acacagatgt
      541 aatgaaaata aagatatttt attgcggccg ctccaagtac ctcccgtacc ttaatattac
      601 ttacttatca tggtagcttg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt
      661 cccaacagtt gcgcagcctg aatggcgaat ggcaaattgt aagcgttaat attttgttaa
      721 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      781 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      841 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa ccgtctatca
      901 agggcgatgg cccactacgt gaaccatcac cctaatcaag tttttttggg cangctagat
      961 acg
//
