LOCUS       sequence-36526-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Lindsey-Boltz LA, Sancar A
  TITLE     Tethering DNA Damage Checkpoint Mediator Proteins Topoisomerase 
            II{beta}-binding Protein 1 (TopBP1) and Claspin to DNA Activates 
            Ataxia-Telangiectasia Mutated and RAD3-related (ATR) Phosphorylation
            of Checkpoint Kinase 1 (Chk1).
  JOURNAL   J Biol Chem. 2011 Jun 3. 286(22):19229-36.
  PUBMED    21502314
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jun 3. 286(22):19229-36."
COMMENT     Sequence Label: pcDNA5-FRT/TO-LacR-TopBP1
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(201..220)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cgngggcntt agcgagctct cctatagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 taccatgcca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
      241 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
      301 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
      361 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
      421 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
      481 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
      541 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
      601 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
      661 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
      721 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
      781 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
      841 gagtgccatg tccggttttc aacaaaccat gcaatgctga atgagggcat cgttcccact
      901 gcgatgctgg ttgccaacga tcanatggcg ctgggcgcaa tgcgcncata caatcggtgc
      961 gtgggt
//
