LOCUS       sequence-36529-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Lindsey-Boltz LA, Sancar A
  TITLE     Tethering DNA Damage Checkpoint Mediator Proteins Topoisomerase 
            II{beta}-binding Protein 1 (TopBP1) and Claspin to DNA Activates 
            Ataxia-Telangiectasia Mutated and RAD3-related (ATR) Phosphorylation
            of Checkpoint Kinase 1 (Chk1).
  JOURNAL   J Biol Chem. 2011 Jun 3. 286(22):19229-36.
  PUBMED    21502314
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jun 3. 286(22):19229-36."
COMMENT     Sequence Label: pcDNA3-LacR-TopBP1
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(113..132)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cgggggctnt agcagactct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag cttaccatgc cagtaacgtt atacgatgtc
      121 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
      181 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
      241 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
      301 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
      361 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
      421 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
      481 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat
      541 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg
      601 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
      661 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
      721 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
      781 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
      841 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgtnggtgc ggatatctcg
      901 gtagtgggat acgacgatac cgaaaagtag tnncgctacc ta
//
