LOCUS       sequence-365302-        9529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of TRIP13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Mittal K, Cooper GW, Lee BP, Su Y, Skinner KT, Shim J, Jonus HC, Kim
            WJ, Doshi M, Almanza D, Kynnap BD, Christie AL, Yang X, Cowley GS, 
            Leeper BA, Morton CL, Dwivedi B, Lawrence T, Rupji M, Keskula P, 
            Meyer S, Clinton CM, Bhasin M, Crompton BD, Tseng YY, Boehm JS, 
            Ligon KL, Root DE, Murphy AJ, Weinstock DM, Gokhale PC, Spangle JM, 
            Rivera MN, Mullen EA, Stegmaier K, Goldsmith KC, Hahn WC, Hong AL
  TITLE     Targeting TRIP13 in favorable histology Wilms tumor with nuclear 
            export inhibitors synergizes with doxorubicin.
  JOURNAL   Commun Biol. 2024 Apr 8;7(1):426. doi: 10.1038/s42003-024-06140-6.
  PUBMED    38589567
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-04-8"; volume: "7(1):426. doi"; pages: " 
            10.1038/s42003-024-06140-6"
COMMENT     Sequence Label: pLX311_TRIP13
FEATURES             Location/Qualifiers
     source          1..9529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    424..445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(460..465,466..483,484..490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    498..514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     503..525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     522..538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     522..538
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     557..577
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        559..577
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        605..831
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2370..2580
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2392..2588
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2439..2574
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2610..3005
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3079..3196
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3334..4512
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3565..4503
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4460..4480
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4532..4556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5862..5886)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5894..5935
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5986..6574
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6039..6059)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6457..6468)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6646..6879
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6957..7078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6994..7013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7048..7067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7118..7253
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7273..7292)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7274..7292)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7302..7319)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7302..7318)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7311..7333)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7460..7915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7547..7566)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7757..7778
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7941..8045
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8046..8114,8115..8906)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8264..8283)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9077..9529,1..136)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
       61 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      361 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      421 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      481 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
      541 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
      601 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
      661 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      721 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      781 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     2341 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     2401 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2461 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2521 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2581 tttgcaaaaa gctttctaga ggtaccacca tggccaagcc tttgtctcaa gaagaatcca
     2641 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     2701 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     2761 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     2821 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     2881 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     2941 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     3001 agggcctgca gctgcagtag taaggcgcgc cgttaacgaa ttctagatct tgagacaaat
     3061 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     3121 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     3181 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     3241 cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     3301 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     3361 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     3421 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     3481 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     3541 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     3601 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     3661 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     3721 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     3781 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     3841 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     3901 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     3961 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     4021 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     4081 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     4141 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     4201 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     4261 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     4321 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     4381 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     4441 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     4501 caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag caggctccac
     4561 catggacgag gccgtgggcg acctgaagca ggcgcttccc tgtgtggccg agtcgccaac
     4621 ggtccacgtg gaggtgcatc agcgcggcag cagcactgca aagaaagaag acataaacct
     4681 gagtgttaga aagctactca acagacataa tattgtgttt ggtgattaca catggactga
     4741 gtttgatgaa ccttttttga ccagaaatgt gcagtctgtg tctattattg acacagaatt
     4801 aaaggttaaa gactcacagc ccatcgattt gagtgcatgc actgttgcac ttcacatttt
     4861 ccagctgaat gaagatggcc ccagcagtga aaatctggag gaagagacag aaaacataat
     4921 tgcagcaaat cactgggttc tacctgcagc tgaattccat gggctttggg acagcttggt
     4981 atacgatgtg gaagtcaaat cccatctcct cgattatgtg atgacaactt tactgttttc
     5041 agacaagaac gtcaacagca acctcatcac ctggaaccgg gtggtgctgc tccacggtcc
     5101 tcctggcact ggaaaaacat ccctgtgtaa agcgttagcc cagaaattga caattagact
     5161 ttcaagcagg taccgatatg gccaattaat tgaaataaac agccacagcc tcttttctaa
     5221 gtggttttcg gaaagtggca agctggtaac caagatgttt cagaagattc aggatttgat
     5281 tgatgataaa gacgccctgg tgttcgtgct gattgatgag gtggagagtc tcacagccgc
     5341 ccgaaatgcc tgcagggcgg gcaccgagcc atcagatgcc atccgcgtgg tcaatgctgt
     5401 cttgacccaa attgatcaga ttaaaaggca ttccaatgtt gtgattctga ccacttctaa
     5461 catcaccgag aagatcgacg tggccttcgt ggacagggct gacatcaagc agtacattgg
     5521 gccaccctct gcagcagcca tcttcaaaat ctacctctct tgtttggaag aactgatgaa
     5581 gtgtcagatc atataccctc gccagcagct gctgaccctc cgagagctag agatgattgg
     5641 cttcattgaa aacaacgtgt caaaattgag ccttcttttg aatgacattt caaggaagag
     5701 cgagggcctc agcggccggg tcctgagaaa actccccttt ctggctcatg cgctgtatgt
     5761 ccaggccccc accgtcacca tagaggggtt cctccaggcc ctgtctctgg cagtggacaa
     5821 gcagtttgaa gagagaaaga agcttgcagc ttacatcttg gacccagctt tcttgtacaa
     5881 agtggttgat atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta
     5941 atgaactagt accggttaag tcgacaatca acgcgttaag tcgacaatca acctctggat
     6001 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6061 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6121 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6181 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6241 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6301 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6361 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6421 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6481 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6541 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     6601 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6661 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6721 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6781 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6841 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     6901 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6961 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7021 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7081 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7141 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7201 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     7261 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     7321 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7381 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7441 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7501 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7561 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7621 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7681 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7741 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7801 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7861 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     7921 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7981 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8041 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8101 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8161 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8221 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8281 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8341 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8401 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8461 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8521 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8581 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8641 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8701 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8761 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8821 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8881 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8941 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9001 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9061 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcag
//
