LOCUS       sequence-365305-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus gRNA targeting human DLAT gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, 
            Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel 
            E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub 
            TR
  TITLE     Copper induces cell death by targeting lipoylated TCA cycle 
            proteins.
  JOURNAL   Science. 2022 Mar 18;375(6586):1254-1261. doi: 
            10.1126/science.abf0529. Epub 2022 Mar 17.
  PUBMED    35298263
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf0529"; journalName: "Science"; date: 
            "2022-03-18- 18"; volume: "375"; issue: "6586"; pages: "1254-1261"
COMMENT     Sequence Label: pXPR_BRD003 DLAT-2
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    84..317
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             502..546
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        724..972
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     724..744
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     895..914
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        994..1069
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    1125..1242
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1381..2559
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1612..2550
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2507..2527
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2572..3171
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2572..2591)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3068..3088
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3187..3775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3240..3260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3658..3669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3847..4080
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4158..4279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4195..4214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4249..4268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4319..4454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4474..4493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4475..4493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4503..4520)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4503..4519)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4512..4534)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4661..5116
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4748..4767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4958..4979
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5142..5246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5247..5315,5316..6107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5465..5484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6278..6866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6767..6786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7020..7037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7154..7175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7190..7195,7196..7213,7214..7220)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7228..7244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7233..7255
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7252..7268
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7252..7268
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7287..7307
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7289..7307
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7335..7561
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7562..7742
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7789..7914
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
       61 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
      121 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
      181 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
      241 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
      301 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
      361 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
      421 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
      481 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
      541 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
      601 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
      661 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
      721 agagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
      781 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
      841 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
      901 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
      961 ggacgaaaca ccgagatggt agaatcacca agagttttag agctagaaat agcaagttaa
     1021 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct
     1081 tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     1141 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     1201 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     1261 agcagagatc cactttggcg ccggctcgag ggggcccggg tgcaaagatg gataaagttt
     1321 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt
     1381 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     1441 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     1501 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     1561 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     1621 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     1681 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     1741 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     1801 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     1861 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     1921 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     1981 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     2041 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     2101 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     2161 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     2221 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     2281 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     2341 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     2401 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     2461 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     2521 gtggttcaaa gtttttttct tccatttcag gtgtcgtgac gtacggccac catgaccgag
     2581 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     2641 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     2701 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     2761 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
     2821 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     2881 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     2941 ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     3001 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     3061 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     3121 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aacgcgttaa
     3181 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3241 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3301 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3361 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3421 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3481 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3541 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3601 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3661 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3721 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     3781 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3841 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3901 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3961 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4021 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4081 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4141 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4201 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4261 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4381 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4441 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4501 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4561 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4621 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4681 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4741 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4801 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4861 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4921 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4981 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5041 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5101 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5161 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5221 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5281 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5341 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5401 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5461 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5521 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5581 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5641 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5701 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5761 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5821 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5881 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5941 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6001 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6061 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6121 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6661 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6841 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6961 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7021 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7081 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7141 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7201 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7261 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7321 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7381 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7441 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7501 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7561 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7621 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7681 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7741 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7801 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7861 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7921 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7981 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8041 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8101 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8161 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8221 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8281 agatatgagg gacaattgga gaagtgaatt atataaatat aaa
//
