LOCUS       sequence-365310-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus gRNA targeting human FDX1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, 
            Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel 
            E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub 
            TR
  TITLE     Copper induces cell death by targeting lipoylated TCA cycle 
            proteins.
  JOURNAL   Science. 2022 Mar 18;375(6586):1254-1261. doi: 
            10.1126/science.abf0529. Epub 2022 Mar 17.
  PUBMED    35298263
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf0529"; journalName: "Science"; date: 
            "2022-03-18- 18"; volume: "375"; issue: "6586"; pages: "1254-1261"
COMMENT     Sequence Label: pXPR_BRD003 FDX1-2
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1946..2401)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2083..2104)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2295..2314
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2528..2550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2542..2559
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2543..2559
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2569..2588
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2569..2587
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2608..2743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2783..2904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2794..2813)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2848..2867
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2982..3215
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3287..3875
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3393..3404
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3802..3822
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3891..4490)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3974..3994)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4471..4490
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4503..5681)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4512..5450
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4535..4555)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5820..5937
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(5993..6068)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(6090..6338)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6148..6167)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6318..6338)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(6516..6560)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6745..6978
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7471..7596
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7643..7823
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7824..8050)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8078..8098)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8078..8096)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8117..8133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8117..8133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8130..8152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8141..8157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8165..8171,8172..8189,8190..8195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8210..8231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     1981 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2041 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2101 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2161 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2221 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2281 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2341 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     2401 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     2461 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     2521 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
     2581 ctatagggcg aattgggtac gtccctaggc ctccaaaaaa gcctcctcac tacttctgga
     2641 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     2701 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     2761 ggatagctag agccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     2821 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     2881 attataagct gcaataaaca agttcctctc actctctgat attcatttct ttgcaagtta
     2941 taaatactga ataataagat gacatgaact actatacgta ctgctagaga ttttccacac
     3001 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     3061 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     3121 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag
     3181 atcttgtctt cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg
     3241 gctaagatct acagctgcct tgtaagtcat tggtcttaaa gtcgacgcgg ggaggcggcc
     3301 caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc
     3361 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
     3421 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
     3481 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
     3541 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
     3601 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
     3661 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     3721 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     3781 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     3841 aatctttcac aaattttgta atccagaggt tgattgtcga cttaacgcgt tcaggcaccg
     3901 ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg
     3961 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg
     4021 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
     4081 ttgccctggt ggtcgggcga gactccgacg gtggccagga accacgcggg ctccttgggc
     4141 cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac
     4201 tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg
     4261 gtccagaccg ccacggcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg
     4321 acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg
     4381 acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg
     4441 gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggtggccgta
     4501 cgtcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg
     4561 aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct
     4621 ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc
     4681 ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc
     4741 gcccggtact ccgtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt
     4801 tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctccct
     4861 gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc
     4921 cttgccgccc agggcggggc gatacacggc ggcgcgaggc caggcaccag agcaggccgg
     4981 ccagcttgag actacccccg tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc
     5041 gaacatgtgc gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata
     5101 ccagtgtgca gatcttggcc cgcatttaca agactatctt gccagaaaaa aagcgtcgca
     5161 gcaggtcatc aaaaatttta aatggctaga gacttatcga aagcagcgag acaggcgcga
     5221 aggtgccacc agattcgcac gcggcggccc cagcgcccag gccaggcctc aactcaagca
     5281 cgaggcgaag gggctcctta agcgcaaggc ctcgaactct cccacccact tccaacccga
     5341 agctcgggat caagaatcac gtactgcagc caggtggaag taattcaagg cacgcaaggg
     5401 ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg
     5461 gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct
     5521 cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg
     5581 cgccaccttc tctaggcacc ggatcaattg ccgacccctc cccccaactt ctcggggact
     5641 gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc caattcccac tcctttcaag
     5701 acctagaagg tccattagct gcaaagattc ctctctgttt aaaactttat ccatctttgc
     5761 acccgggccc cctcgagccg gcgccaaagt ggatctctgc tgtccctgta ataaacccga
     5821 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt
     5881 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg
     5941 tggatgaata ctgccatttg tctcaagatc tagttacgcc aagcttaaaa aagcaccgac
     6001 tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc
     6061 tctaaaacgt ctgttagtcc atatgccacg gtgtttcgtc ctttccacaa gatatataaa
     6121 gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat
     6181 aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact
     6241 aatatctttg tgtttacagt caaattaatt ctaattatct ctctaacagc cttgtatcgt
     6301 atatgcaaat atgaaggaat catgggaaat aggccctctc tgggtcccct cggggttggg
     6361 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     6421 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     6481 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     6541 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     6601 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     6661 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     6721 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     6781 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     6841 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     6901 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     6961 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     7021 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     7081 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg
     7141 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     7201 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     7261 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     7321 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     7381 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     7441 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     7501 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     7561 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     7621 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     7681 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     7741 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     7801 atctggtcta accagagaga cccgtttatg tatcgagcta ggcacttaaa tacaatatct
     7861 ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca gacccgtctg ttgccttcct
     7921 aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg ctttttctct
     7981 ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa gagtattgca
     8041 taagactaca ttaagcttgc agctccagct tttgttccct ttagtgaggg ttaattgcgc
     8101 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg ttt
//
