LOCUS       sequence-365311-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus gRNA targeting human LIPT2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, 
            Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel 
            E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub 
            TR
  TITLE     Copper induces cell death by targeting lipoylated TCA cycle 
            proteins.
  JOURNAL   Science. 2022 Mar 18;375(6586):1254-1261. doi: 
            10.1126/science.abf0529. Epub 2022 Mar 17.
  PUBMED    35298263
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf0529"; journalName: "Science"; date: 
            "2022-03-18- 18"; volume: "375"; issue: "6586"; pages: "1254-1261"
COMMENT     Sequence Label: pXPR_BRD003 LIPT2-2
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    197..318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(234..253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     288..307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      358..493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(513..532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(514..532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(542..559)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(542..558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(551..573)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      700..1155
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(787..806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     997..1018
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1181..1285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1286..1354,1355..2146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1504..1523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2317..2905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2806..2825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3059..3076
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3193..3214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3229..3234,3235..3252,3253..3259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3267..3283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3272..3294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3291..3307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3291..3307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3326..3346
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3328..3346
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3374..3600
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3601..3781
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3828..3953
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4446..4679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4864..4908
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5086..5334
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5086..5106
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5257..5276
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5356..5431
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5487..5604
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5743..6921
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5974..6912
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6869..6889
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6934..7533
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6934..6953)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7430..7450
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7549..8137
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7602..7622)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8020..8031)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8209..8323,1..119)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
       61 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      121 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
      181 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      241 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
      301 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
      361 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      421 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
      481 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      541 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      601 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      661 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      721 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      781 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      841 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      901 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      961 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1021 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1081 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1141 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1201 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1261 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1321 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1381 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1441 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1501 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1561 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1621 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1681 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1741 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1801 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1861 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1921 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1981 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2041 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2101 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2161 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2221 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2281 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2341 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2401 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2461 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2521 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2581 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2641 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2701 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2761 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2821 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2881 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2941 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3001 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3061 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3121 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3181 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     3241 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3301 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     3361 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3421 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3481 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3541 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     3601 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3661 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3721 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3781 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3841 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3901 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3961 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     4021 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     4081 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     4141 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     4201 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     4261 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     4321 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     4381 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4441 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4501 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4561 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4621 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4681 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     4741 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4801 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4861 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4921 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4981 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     5041 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc
     5101 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     5161 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     5221 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     5281 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcatgc
     5341 acgtagcgtc gctgggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     5401 tcaacttgaa aaagtggcac cgagtcggtg cttttttaag cttggcgtaa ctagatcttg
     5461 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     5521 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     5581 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     5641 cgccggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt
     5701 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     5761 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5821 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5881 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5941 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     6001 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     6061 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     6121 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     6181 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     6241 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     6301 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     6361 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6421 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6481 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6541 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     6601 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     6661 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     6721 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     6781 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     6841 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     6901 cttccatttc aggtgtcgtg acgtacggcc accatgaccg agtacaagcc cacggtgcgc
     6961 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     7021 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     7081 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     7141 ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     7201 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     7261 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcgga
     7321 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     7381 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     7441 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     7501 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg
     7561 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7621 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7681 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7741 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7801 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7861 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7921 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7981 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8041 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8101 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     8161 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8221 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8281 agatctgagc ctgggagctc tctggctaac tagggaaccc act
//
