LOCUS       sequence-365313-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus gRNA targeting Ch2-2 (control) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, 
            Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel 
            E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub 
            TR
  TITLE     Copper induces cell death by targeting lipoylated TCA cycle 
            proteins.
  JOURNAL   Science. 2022 Mar 18;375(6586):1254-1261. doi: 
            10.1126/science.abf0529. Epub 2022 Mar 17.
  PUBMED    35298263
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf0529"; journalName: "Science"; date: 
            "2022-03-18- 18"; volume: "375"; issue: "6586"; pages: "1254-1261"
COMMENT     Sequence Label: pXPR_BRD003 Ch2-2
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    244..365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(281..300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     335..354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      405..540
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(560..579)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(561..579)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(589..606)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(589..605)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(598..620)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      747..1202
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(834..853)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1044..1065
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1228..1332
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1333..1401,1402..2193)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1551..1570)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2364..2952
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2853..2872
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3106..3123
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3240..3261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3276..3281,3282..3299,3300..3306)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3314..3330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3319..3341
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3338..3354
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3338..3354
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3373..3393
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3375..3393
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3421..3647
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3648..3828
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3875..4000
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4493..4726
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4911..4955
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5133..5381
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5133..5153
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5304..5323
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5403..5478
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5534..5651
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5790..6968
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6021..6959
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6916..6936
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6981..7580
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6981..7000)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7477..7497
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7596..8184
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7649..7669)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8067..8078)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8256..8323,1..166)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
       61 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
      181 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
      241 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      301 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
      361 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
      421 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
      481 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      541 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
      601 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      661 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      721 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      781 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      841 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      901 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      961 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     1021 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     1081 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     1141 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     1201 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1261 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1321 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1381 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1441 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1501 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1561 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1621 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1681 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1741 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1801 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1861 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1921 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1981 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2041 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2101 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2161 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2221 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2281 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2341 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2401 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2461 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2521 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2581 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2641 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2701 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2761 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2821 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2881 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2941 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3001 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3061 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3121 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3181 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3241 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     3301 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     3361 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     3421 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     3481 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     3541 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     3601 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     3661 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3721 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3781 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     3841 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     3901 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     3961 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     4021 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     4081 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     4141 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     4201 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     4261 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     4321 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     4381 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     4441 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     4501 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     4561 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     4621 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     4681 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     4741 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     4801 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     4861 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     4921 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     4981 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     5041 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     5101 agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat
     5161 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     5221 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     5281 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     5341 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgggtgtgcg tatgaagcag
     5401 tggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     5461 gtggcaccga gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga caaatggcag
     5521 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     5581 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     5641 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     5701 gggcccgggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
     5761 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     5821 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga
     5881 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     5941 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     6001 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     6061 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     6121 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     6181 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     6241 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     6301 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     6361 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     6421 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     6481 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     6541 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     6601 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     6661 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     6721 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     6781 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     6841 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     6901 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     6961 tgtcgtgacg tacggccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7021 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7081 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     7141 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg
     7201 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7261 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     7321 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc
     7381 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     7441 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     7501 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     7561 cccgcaagcc cggtgcctga acgcgttaag tcgacaatca acctctggat tacaaaattt
     7621 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7681 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7741 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7801 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7861 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7921 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7981 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     8041 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     8101 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     8161 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     8221 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8281 aagacaagat ctgctttttg cttgtactgg gtctctctgg tta
//
