LOCUS       sequence-365314-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus gRNA targeting human LIPT1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, 
            Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel 
            E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub 
            TR
  TITLE     Copper induces cell death by targeting lipoylated TCA cycle 
            proteins.
  JOURNAL   Science. 2022 Mar 18;375(6586):1254-1261. doi: 
            10.1126/science.abf0529. Epub 2022 Mar 17.
  PUBMED    35298263
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf0529"; journalName: "Science"; date: 
            "2022-03-18- 18"; volume: "375"; issue: "6586"; pages: "1254-1261"
COMMENT     Sequence Label: pXPR_BRD003 LIPT1-1
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    249..370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(286..305)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     340..359
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      410..545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(565..584)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(566..584)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(594..611)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(594..610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(603..625)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      752..1207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(839..858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1049..1070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1233..1337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1338..1406,1407..2198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1556..1575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2369..2957
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2858..2877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3111..3128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3245..3266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3281..3286,3287..3304,3305..3311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3319..3335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3324..3346
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3343..3359
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3343..3359
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3378..3398
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3380..3398
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3426..3652
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3653..3833
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3880..4005
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4498..4731
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4916..4960
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5138..5386
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5138..5158
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5309..5328
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5408..5483
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5539..5656
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5795..6973
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6026..6964
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6921..6941
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6986..7585
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6986..7005)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7482..7502
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7601..8189
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7654..7674)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8072..8083)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8261..8323,1..171)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
       61 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      121 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
      181 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
      241 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      301 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      361 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
      421 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      481 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      541 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
      601 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      661 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      721 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      781 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      841 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      901 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      961 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     1021 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1081 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     1141 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     1201 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1261 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1321 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1381 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1441 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1501 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1561 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1621 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1681 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1741 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1801 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1861 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1921 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1981 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2041 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2101 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2161 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2221 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2281 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2341 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2401 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2461 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2521 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2581 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2641 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2701 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2761 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2821 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2881 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2941 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3001 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3061 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3121 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3181 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3241 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     3301 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     3361 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     3421 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     3481 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     3541 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     3601 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     3661 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     3721 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     3781 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     3841 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     3901 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     3961 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     4021 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     4081 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     4141 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     4201 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     4261 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     4321 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     4381 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     4441 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     4501 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     4561 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     4621 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     4681 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     4741 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     4801 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     4861 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     4921 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     4981 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5041 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5101 gtttcagacc cacctcccaa ccccgagggg acccagagag ggcctatttc ccatgattcc
     5161 ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt
     5221 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
     5281 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
     5341 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgatg cctaccaatt
     5401 acaacaggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     5461 aaaaagtggc accgagtcgg tgctttttta agcttggcgt aactagatct tgagacaaat
     5521 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     5581 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     5641 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     5701 cgagggggcc cgggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     5761 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     5821 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc
     5881 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     5941 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     6001 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     6061 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     6121 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     6181 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     6241 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     6301 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     6361 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     6421 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     6481 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     6541 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     6601 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     6661 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     6721 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     6781 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     6841 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     6901 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     6961 tcaggtgtcg tgacgtacgg ccaccatgac cgagtacaag cccacggtgc gcctcgccac
     7021 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     7081 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     7141 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     7201 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     7261 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     7321 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gagtctcgcc
     7381 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     7441 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     7501 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     7561 catgacccgc aagcccggtg cctgaacgcg ttaagtcgac aatcaacctc tggattacaa
     7621 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7681 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7741 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7801 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7861 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7921 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7981 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     8041 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     8101 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     8161 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     8221 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     8281 caacgaagac aagatctgct ttttgcttgt actgggtctc tct
//
