LOCUS       sequence-365315-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus gRNA targeting human LIPT1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, 
            Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel 
            E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub 
            TR
  TITLE     Copper induces cell death by targeting lipoylated TCA cycle 
            proteins.
  JOURNAL   Science. 2022 Mar 18;375(6586):1254-1261. doi: 
            10.1126/science.abf0529. Epub 2022 Mar 17.
  PUBMED    35298263
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf0529"; journalName: "Science"; date: 
            "2022-03-18- 18"; volume: "375"; issue: "6586"; pages: "1254-1261"
COMMENT     Sequence Label: pXPR_BRD003 LIPT1-2
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    541..666
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             713..893
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(894..1120)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1148..1168)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1148..1166)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1187..1203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1187..1203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1200..1222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1211..1227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1235..1241,1242..1259,1260..1265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1280..1301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1418..1435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1589..2177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1669..1688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2348..3139,3140..3208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2971..2990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3209..3313)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3339..3794)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3476..3497)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3688..3707
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3921..3943
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3935..3952
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3936..3952
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3962..3981
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3962..3980
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4001..4136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4176..4297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4187..4206)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4241..4260
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4375..4608
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4680..5268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4786..4797
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5195..5215
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5284..5883)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5367..5387)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5864..5883
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5896..7074)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5905..6843
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5928..5948)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7213..7330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(7386..7461)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7483..7731)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7541..7560)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7711..7731)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(7909..7953)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(8138..8323,1..48)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
       61 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
      121 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
      181 ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
      241 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
      301 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
      361 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
      421 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
      481 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
      541 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
      601 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
      661 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
      721 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
      781 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
      841 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg
      901 tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca
      961 atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc
     1021 accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt
     1081 accatgttgc aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct
     1141 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     1201 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1261 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1321 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1381 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1441 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1501 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1561 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1621 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1681 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1741 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1801 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1861 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1921 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1981 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2041 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2101 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2161 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2221 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2281 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2341 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2401 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2461 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2521 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2581 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2641 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2701 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2761 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2821 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2881 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2941 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3001 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3061 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3121 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3181 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3241 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3301 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     3361 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3421 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3481 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3541 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3601 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3661 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3721 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3781 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     3841 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     3901 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
     3961 gtaatacgac tcactatagg gcgaattggg tacgtcccta ggcctccaaa aaagcctcct
     4021 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     4081 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     4141 ggagttaggg gcgggatagc tagagccaga catgataaga tacattgatg agtttggaca
     4201 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     4261 tttatttgta accattataa gctgcaataa acaagttcct ctcactctct gatattcatt
     4321 tctttgcaag ttataaatac tgaataataa gatgacatga actactatac gtactgctag
     4381 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     4441 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     4501 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     4561 aagcaaaaag cagatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt
     4621 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaagtcgacg
     4681 cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag
     4741 aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg
     4801 gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg
     4861 aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc
     4921 tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa
     4981 agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc
     5041 gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata
     5101 aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat
     5161 agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt
     5221 aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacttaacg
     5281 cgttcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
     5341 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
     5401 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
     5461 gctgcccaga cccttgccct ggtggtcggg cgagactccg acggtggcca ggaaccacgc
     5521 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
     5581 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
     5641 gctctccggc gtggtccaga ccgccacggc ggcgccgtcg tccgcgaccc acaccttgcc
     5701 gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg
     5761 gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt
     5821 gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt
     5881 catggtggcc gtacgtcacg acacctgaaa tggaagaaaa aaactttgaa ccactgtctg
     5941 aggcttgaga atgaaccaag atccaaactc aaaaagggca aattccaagg agaattacat
     6001 caagtgccaa gctggcctaa cttcagtctc cacccactca gtgtggggaa actccatcgc
     6061 ataaaacccc tccccccaac ctaaagacga cgtactccaa aagctcgaga actaatcgag
     6121 gtgcctggac ggcgcccggt actccgtgga gtcacatgaa gcgacggctg aggacggaaa
     6181 ggcccttttc ctttgtgtgg gtgactcacc cgcccgctct cccgagcgcc gcgtcctcca
     6241 ttttgagctc cctgcagcag ggccgggaag cggccatctt tccgctcacg caactggtgc
     6301 cgaccgggcc agccttgccg cccagggcgg ggcgatacac ggcggcgcga ggccaggcac
     6361 cagagcaggc cggccagctt gagactaccc ccgtccgatt ctcggtggcc gcgctcgcag
     6421 gccccgcctc gccgaacatg tgcgctggga cgcacgggcc ccgtcgccgc ccgcggcccc
     6481 aaaaaccgaa ataccagtgt gcagatcttg gcccgcattt acaagactat cttgccagaa
     6541 aaaaagcgtc gcagcaggtc atcaaaaatt ttaaatggct agagacttat cgaaagcagc
     6601 gagacaggcg cgaaggtgcc accagattcg cacgcggcgg ccccagcgcc caggccaggc
     6661 ctcaactcaa gcacgaggcg aaggggctcc ttaagcgcaa ggcctcgaac tctcccaccc
     6721 acttccaacc cgaagctcgg gatcaagaat cacgtactgc agccaggtgg aagtaattca
     6781 aggcacgcaa gggccataac ccgtaaagag gccaggcccg cgggaaccac acacggcact
     6841 tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga ctactgcact
     6901 tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga catcactttc
     6961 ccagtttacc ccgcgccacc ttctctaggc accggatcaa ttgccgaccc ctccccccaa
     7021 cttctcgggg actgtgggcg atgtgcgctc tgcccactga cgggcaccgg agccaattcc
     7081 cactcctttc aagacctaga aggtccatta gctgcaaaga ttcctctctg tttaaaactt
     7141 tatccatctt tgcacccggg ccccctcgag ccggcgccaa agtggatctc tgctgtccct
     7201 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     7261 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     7321 tcttttaaaa ttgtggatga atactgccat ttgtctcaag atctagttac gccaagctta
     7381 aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattttaact
     7441 tgctatttct agctctaaaa cctgctagca taccttcctt acggtgtttc gtcctttcca
     7501 caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc
     7561 cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca
     7621 cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta tctctctaac
     7681 agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct ctctgggtcc
     7741 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     7801 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     7861 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     7921 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     7981 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     8041 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     8101 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     8161 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     8221 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     8281 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcg
//
