LOCUS       sequence-365332-        1876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-2-adrenergic receptor internally tagged with miRFP670nano3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Oliinyk OS, Baloban M, Clark CL, Carey E, Pletnev S, Nimmerjahn A, 
            Verkhusha VV
  TITLE     Single-domain near-infrared protein provides a scaffold for 
            antigen-dependent fluorescent nanobodies.
  JOURNAL   Nat Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 
            10.1038/s41592-022-01467-6.
  PUBMED    35606446
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 
            10.1038/s41592-022-01467-6."
COMMENT     Sequence Label: pB2AR-miRFP670nano3
FEATURES             Location/Qualifiers
     source          1..1876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1832..1849)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct ggctagcatg gggcaacccg ggaacggcag cgccttcttg ctggcaccca
      121 atagaagcca tgcgccggac cacgacgtca cgcagcaaag ggacgaggtg tgggtggtgg
      181 gcatgggcat cgtcatgtct ctcatcgtcc tggccatcgt gtttggcaat gtgctggtca
      241 tcacagccat tgccaagttc gagcgtctgc agacggtcac caactacttc atcacttcac
      301 tggcctgtgc tgatctggtc atgggcctgg cagtggtgcc ctttggggcc gcccatattc
      361 ttatgaaaat gtggactttt ggcaacttct ggtgcgagtt ttggacttcc attgatgtgc
      421 tgtgcgtcac ggccagcatt gagaccctgt gcgtgatcgc agtggatcgc tactttgcca
      481 ttacttcacc tttcaagtac cagagcctgc tgaccaagaa taaggcccgg gtgatcattc
      541 tgatggtgtg gattgtgtca ggccttacct ccttcttgcc cattcagatg cactggtacc
      601 gggccaccca ccaggaagcc atcaactgct atgccaatga gacctgctgt gacttcttca
      661 cgaaccaagc ctatgccatt gcctcttcca tcgtgtcctt ctacgttccc ctggtgatca
      721 tggtcttcgt ctactccagg gtctttcagg aggccaaaag gcagctccag aagattgaca
      781 aatctgaggg ccgcttccat gtccagaacc ttagccaggt ggagggatcc ggtgggggag
      841 gcagcatggc aaacctggac aagatgctga acaccaccgt gaccgaggtg cgcaagttcc
      901 tgcaagcgga cagagtgtgc gtgttcaagt tcgaggaaga ttactccggc accgtctcgc
      961 acgaagccgt ggacgacaga tggattagca tcctgaagac ccaggtgcag gacagatact
     1021 tcatggaaac cagaggcgag gaatacgtcc acggcagata ccaggccatc gccgacatct
     1081 acacagccaa tctggtcgag tgctacagag acctgctgat cgagtttcag gtgcgggcca
     1141 ttctggctgt ccccatcctg caaggcaaga agctgtgggg cctgctggtg gcccaccaac
     1201 tggccggccc tcgggagtgg cagacctggg aaatcgactt cctgaaacag caagccgtgg
     1261 tgatgggcat cgccatccag cagagcaccg gtcaggatgg gcggacgggg catggactcc
     1321 gcagatcttc caagttctgc ttgaaggagc acaaagccct caagacgtta ggcatcatca
     1381 tgggcacttt caccctctgc tggctgccct tcttcatcgt taacattgtg catgtgatcc
     1441 aggataacct catccgtaag gaagtttaca tcctcctaaa ttggataggc tatgtcaatt
     1501 ctggtttcaa tccccttatc tactgccgga gcccagattt caggattgcc ttccaggagc
     1561 ttctgtgcct gcgcaggtct tctttgaagg cctatgggaa tggctactcc agcaacggca
     1621 acacagggga gcagagtgga tatcacgtgg aacaggagaa agaaaataaa ctgctgtgtg
     1681 aagacctccc aggcacggaa gactttgtgg gccatcaagg tactgtgcct agcgataaca
     1741 ttgattcaca agggaggaat tgtagtacaa atgactcact gctgtaagcg gccgctcgag
     1801 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     1861 tctgttgttt gcccct
//
