LOCUS       sequence-365336-        7206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Csomos RA, Wright CW, Galban S, Oetjen KA, Duckett CS
  TITLE     Two distinct signalling cascades target the NF-kappaB regulatory 
            factor c-IAP1 for degradation.
  JOURNAL   Biochem J. 2009 Apr 28;420(1):83-91.
  PUBMED    19243308
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "2009-04-28"; volume: "420(1)"; pages: "83-91"
COMMENT     Sequence Label: pEBB HA cIAP1
FEATURES             Location/Qualifiers
     source          1..7206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..27)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10..26)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19..41)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      239..694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(326..345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     536..557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(711..730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     830..852
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(890..908)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        976..1080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1081..1149,1150..1941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1299..1318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2112..2700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2601..2620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2854..2871
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2988..3009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3024..3029,3030..3047,3048..3054)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3062..3078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3067..3089
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3086..3102
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3086..3102
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3134..3344
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3156..3352
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3203..3338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3439..4617
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3670..4608
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4565..4585
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4682..4708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4688..4714
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 accgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
       61 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
      181 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
      241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      301 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      421 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
      481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      541 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
      601 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
      661 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
      721 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
      781 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      841 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
      901 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      961 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1021 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1081 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1141 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1201 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1261 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1321 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1381 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1441 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1501 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1561 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1621 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1681 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1741 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1801 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1861 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1921 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1981 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2041 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2221 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2761 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2821 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2881 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     2941 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     3001 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     3061 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
     3121 aattccctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3181 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3241 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3301 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttg
     3361 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     3421 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     3481 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     3541 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     3601 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     3661 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     3721 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     3781 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     3841 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     3901 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     3961 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     4021 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     4081 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     4141 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     4201 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     4261 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     4321 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     4381 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     4441 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     4501 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     4561 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     4621 attctctaga gatccctcga cctcgagatc cattgtgctg gatctcacca tggcttctag
     4681 ctatccttat gacgtgcctg actatgccag cctgggatcc atgcacaaaa ctgcctccca
     4741 aagacttttc ccaggtccct cgtatcaaaa cattaagagt ataatggaag atagcacgat
     4801 cttgtcagat tggacaaaca gcaacaaaca aaaaatgaag tatgactttt cctgtgaact
     4861 ctacagaatg tctacatatt caactttccc cgccggggtg cctgtctcag aaaggagtct
     4921 tgctcgtgct ggtttttatt atactggtgt gaatgacaag gtcaaatgct tctgttgtgg
     4981 cctgatgctg gataactgga aactaggaga cagtcctatt caaaagcata aacagctata
     5041 tcctagctgt agctttattc agaatctggt ttcagctagt ctgggatcca cctctaagaa
     5101 tacgtctcca atgagaaaca gttttgcaca ttcattatct cccaccttgg aacatagtag
     5161 cttgttcagt ggttcttact ccagcctttc tccaaaccct cttaattcta gagcagttga
     5221 agacatctct tcatcgagga ctaaccccta cagttatgca atgagtactg aagaagccag
     5281 atttcttacc taccatatgt ggccattaac ttttttgtca ccatcagaat tggcaagagc
     5341 tggtttttat tatataggac ctggagatag ggtagcctgc tttgcctgtg gtgggaagct
     5401 cagtaactgg gaaccaaagg atgatgctat gtcagaacac cggaggcatt ttcccaactg
     5461 tccatttttg gaaaattctc tagaaactct gaggtttagc atttcaaatc tgagcatgca
     5521 gacacatgca gctcgaatga gaacatttat gtactggcca tctagtgttc cagttcagcc
     5581 tgagcagctt gcaagtgctg gtttttatta tgtgggtcgc aatgatgatg tcaaatgctt
     5641 ttgttgtgat ggtggcttga ggtgttggga atctggagat gatccatggg tagaacatgc
     5701 caagtggttt ccaaggtgtg agttcttgat acgaatgaaa ggccaagagt ttgttgatga
     5761 gattcaaggt agatatcctc atcttcttga acagctgttg tcaacttcag ataccactgg
     5821 agaagaaaat gctgacccac caattattca ttttggacct ggagaaagtt cttcagaaga
     5881 tgctgtcatg atgaatacac ctgtggttaa atctgccttg gaaatgggct ttaatagaga
     5941 cctggtgaaa caaacagttc aaagtaaaat cctgacaact ggagagaact ataaaacagt
     6001 taatgatatt gtgtcagcac ttcttaatgc tgaagatgaa aaaagagaag aggagaagga
     6061 aaaacaagct gaagaaatgg catcagatga tttgtcatta attcggaaga acagaatggc
     6121 tctctttcaa caattgacat gtgtgcttcc tatcctggat aatcttttaa aggccaatgt
     6181 aattaataaa caggaacatg atattattaa acaaaaaaca cagatacctt tacaagcgag
     6241 agaactgatt gataccattt tggttaaagg aaatgctgcg gccaacatct tcaaaaactg
     6301 tctaaaagaa attgactcta cattgtataa gaacttattt gtggataaga atatgaagta
     6361 tattccaaca gaagatgttt caggtctgtc actggaagaa caattgagga ggttgcaaga
     6421 agaacgaact tgtaaagtgt gtatggacaa agaagtttct gttgtattta ttccttgtgg
     6481 tcatctggta gtatgccagg aatgtgcccc ttctctaaga aaatgcccta tttgcagggg
     6541 tataatcaag ggtactgttc gtacatttct ctcttaaggt caccagatct agtaagccga
     6601 attctgcaga tatccatcac actggcggcc gcgactctag agtgagggtc cccacctggg
     6661 acccttgaga gtatcaggtc tcccacgtgg gagacaagaa atccctgttt aatatttaaa
     6721 cagcagtgtt ccccatctgg gtccttgcac ccctcactct ggcctcagcc gactgcacag
     6781 cggcccctgc atccccttgg ctgtgaggcc cctggacaag cagaggtggc cagagctggg
     6841 aggcatggcc ctggggtccc acgaatttgc tggggaatct cgtttttctt cttaagactt
     6901 ttgggacatg gtttgactcc cgaacatcac cgacgtgtct cctgtttttc tgggtggcct
     6961 cgggacacct gccctgcccc cacgagggtc aggactgtga ctctttttag ggccaggcag
     7021 gtgcctggac atttgccttg ctggacgggg actggggatg tgggagggag cagacaggag
     7081 gaatcatgtc aggcctgtgt gtgaaaggaa gctccactgt caccctccac ctcttcaccc
     7141 cccactcacc agtgtcccct ccactgtcac attgtaactg aacttcagga taataaagtg
     7201 tttgcc
//
