LOCUS       sequence-365346-        4137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGF receptor (EGFR) labelled with near-infrared fluorescent protein 
            miRFP670nano3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Oliinyk OS, Baloban M, Clark CL, Carey E, Pletnev S, Nimmerjahn A, 
            Verkhusha VV
  TITLE     Single-domain near-infrared protein provides a scaffold for 
            antigen-dependent fluorescent nanobodies.
  JOURNAL   Nat Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 
            10.1038/s41592-022-01467-6.
  PUBMED    35606446
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 
            10.1038/s41592-022-01467-6."
COMMENT     Sequence Label: pEGFR-miRFP670nano3
FEATURES             Location/Qualifiers
     source          1..4137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(16..87,88..1950,1951..2019,2020..3645)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     regulatory      3670..3679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cttcggggag cagcgatgcg accctccggg acggccgggg cagcgctcct ggcgctgctg
       61 gctgcgctct gcccggcgag tcgggctctg gaggaaaaga aagtttgcca aggcacgagt
      121 aacaagctca cgcagttggg cacttttgaa gatcattttc tcagcctcca gaggatgttc
      181 aataactgtg aggtggtcct tgggaatttg gaaattacct atgtgcagag gaattatgat
      241 ctttccttct taaagaccat ccaggaggtg gctggttatg tcctcattgc cctcaacaca
      301 gtggagcgaa ttcctttgga aaacctgcag atcatcagag gaaatatgta ctacgaaaat
      361 tcctatgcct tagcagtctt atctaactat gatgcaaata aaaccggact gaaggagctg
      421 cccatgagaa atttacagga aatcctgcat ggcgccgtgc ggttcagcaa caaccctgcc
      481 ctgtgcaacg tggagagcat ccagtggcgg gacatagtca gcagtgactt tctcagcaac
      541 atgtcgatgg acttccagaa ccacctgggc agctgccaaa agtgtgatcc aagctgtccc
      601 aatgggagct gctggggtgc aggagaggag aactgccaga aactgaccaa aatcatctgt
      661 gcccagcagt gctccgggcg ctgccgtggc aagtccccca gtgactgctg ccacaaccag
      721 tgtgctgcag gctgcacagg cccccgggag agcgactgcc tggtctgccg caaattccga
      781 gacgaagcca cgtgcaagga cacctgcccc ccactcatgc tctacaaccc caccacgtac
      841 cagatggatg tgaaccccga gggcaaatac agctttggtg ccacctgcgt gaagaagtgt
      901 ccccgtaatt atgtggtgac agatcacggc tcgtgcgtcc gagcctgtgg ggccgacagc
      961 tatgagatgg aggaagacgg cgtccgcaag tgtaagaagt gcgaagggcc ttgccgcaaa
     1021 gtgtgtaacg gaataggtat tggtgaattt aaagactcac tctccataaa tgctacgaat
     1081 attaaacact tcaaaaactg cacctccatc agtggcgatc tccacatcct gccggtggca
     1141 tttaggggtg actccttcac acatactcct cctctggatc cacaggaact ggatattctg
     1201 aaaaccgtaa aggaaatcac agggtttttg ctgattcagg cttggcctga aaacaggacg
     1261 gacctccatg cctttgagaa cctagaaatc atacgcggca ggaccaagca acatggtcag
     1321 ttttctcttg cagtcgtcag cctgaacata acatccttgg gattacgctc cctcaaggag
     1381 ataagtgatg gagatgtgat aatttcagga aacaaaaatt tgtgctatgc aaatacaata
     1441 aactggaaaa aactgtttgg gacctccggt cagaaaacca aaattataag caacagaggt
     1501 gaaaacagct gcaaggccac aggccaggtc tgccatgcct tgtgctcccc cgagggctgc
     1561 tggggcccgg agcccaggga ctgcgtctct tgccggaatg tcagccgagg cagggaatgc
     1621 gtggacaagt gcaaccttct ggagggtgag ccaagggagt ttgtggagaa ctctgagtgc
     1681 atacagtgcc acccagagtg cctgcctcag gccatgaaca tcacctgcac aggacgggga
     1741 ccagacaact gtatccagtg tgcccactac attgacggcc cccactgcgt caagacctgc
     1801 ccggcaggag tcatgggaga aaacaacacc ctggtctgga agtacgcaga cgccggccat
     1861 gtgtgccacc tgtgccatcc aaactgcacc tacggatgca ctgggccagg tcttgaaggc
     1921 tgtccaacga atgggcctaa gatcccgtcc atcgccactg ggatggtggg ggccctcctc
     1981 ttgctgctgg tggtggccct ggggatcggc ctcttcatgc gaaggcgcca catcgttcgg
     2041 aagcgcacgc tgcggaggct gctgcaggag agggagcttg tggagcctct tacacccagt
     2101 ggagaagctc ccaaccaagc tctcttgagg atcttgaagg aaactgaatt caaaaagatc
     2161 aaagtgctgg gctccggtgc gttcggcacg gtgtataagg gactctggat cccagaaggt
     2221 gagaaagtta aaattcccgt cgctatcaag gaattaagag aagcaacatc tccgaaagcc
     2281 aacaaggaaa tcctcgatga agcctacgtg atggccagcg tggacaaccc ccacgtgtgc
     2341 cgcctgctgg gcatctgcct cacctccacc gtgcaactca tcacgcagct catgcccttc
     2401 ggctgcctcc tggactatgt ccgggaacac aaagacaata ttggctccca gtacctgctc
     2461 aactggtgtg tgcagatcgc aaagggcatg aactacttgg aggaccgtcg cttggtgcac
     2521 cgcgacctgg cagccaggaa cgtactggtg aaaacaccgc agcatgtcaa gatcacagat
     2581 tttgggctgg ccaaactgct gggtgcggaa gagaaagaat accatgcaga aggaggcaaa
     2641 gtgcctatca agtggatggc attggaatca attttacaca gaatctatac ccaccagagt
     2701 gatgtctgga gctacggggt gaccgtttgg gagttgatga cctttggatc caagccatat
     2761 gacggaatcc ctgccagcga gatctcctcc atcctggaga aaggagaacg cctccctcag
     2821 ccacccatat gtaccatcga tgtctacatg atcatggtca agtgctggat gatagacgca
     2881 gatagtcgcc caaagttccg tgagttgatc atcgaattct ccaaaatggc ccgagacccc
     2941 cagcgctacc ttgtcattca gggggatgaa agaatgcatt tgccaagtcc tacagactcc
     3001 aacttctacc gtgccctgat ggatgaagaa gacatggacg acgtggtgga tgccgacgag
     3061 tacctcatcc cacagcaggg cttcttcagc agcccctcca cgtcacggac tcccctcctg
     3121 agctctctga gtgcaaccag caacaattcc accgtggctt gcattgatag aaatgggctg
     3181 caaagctgtc ccatcaagga agacagcttc ttgcagcgat acagctcaga ccccacaggc
     3241 gccttgactg aggacagcat agacgacacc ttcctcccag tgcctgaata cataaaccag
     3301 tccgttccca aaaggcccgc tggctctgtg cagaatcctg tctatcacaa tcagcctctg
     3361 aaccccgcgc ccagcagaga cccacactac caggaccccc acagcactgc agtgggcaac
     3421 cccgagtatc tcaacactgt ccagcccacc tgtgtcaaca gcacattcga cagccctgcc
     3481 cactgggccc agaaaggcag ccaccaaatt agcctggaca accctgacta ccagcaggac
     3541 ttctttccca aggaagccaa gccaaatggc atctttaagg gctccacagc tgaaaatgca
     3601 gaatacctaa gggtcgcgcc acaaagcagt gaatttattg gagcaccgcg ggcccgggat
     3661 ccaccggtcg ccaccatggc aaacctggac aagatgctga acaccaccgt gaccgaggtg
     3721 cgcaagttcc tgcaagcgga cagagtgtgc gtgttcaagt tcgaggaaga ttactccggc
     3781 accgtctcgc acgaagccgt ggacgacaga tggattagca tcctgaagac ccaggtgcag
     3841 gacagatact tcatggaaac cagaggcgag gaatacgtcc acggcagata ccaggccatc
     3901 gccgacatct acacagccaa tctggtcgag tgctacagag acctgctgat cgagtttcag
     3961 gtgcgggcca ttctggctgt ccccatcctg caaggcaaga agctgtgggg cctgctggtg
     4021 gcccaccaac tggccggccc tcgggagtgg cagacctggg aaatcgactt cctgaaacag
     4081 caagccgtgg tgatgggcat cgccatccag cagagctagg cggccgcgac tctagat
//
