LOCUS       sequence-36546-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL Traffic Light Reporter 1.1 (Sce target) E...
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..88
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             89..796
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(231..249)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     499..518
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tctggctgag actgtacaag taactggatc cggtgagggc agaggaagtc ttctaacatg
       61 cggtgacgtg gaggagaatc cgggccctgt gagcaagggc gaggaggata actccgccat
      121 catcaaggag ttcctgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      181 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      241 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
      301 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
      361 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      421 gacccaggac tcctctctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      481 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
      541 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
      601 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
      661 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
      721 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
      781 cgagctgtac aagtgaatgc agaggctccg gtgcccgtca gtgggcagag cgcacatcgc
      841 ccacagtccc cgagaagttg gggggaaggg gtcggcaatt gaaccggtgc ctagagaa
//
