LOCUS       sequence-365498-       10229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse Nrx3-alpha lacking insertion at the alternative 
            splice site 4 together with EGFP protein separated by 2A peptide. 
            Contains insertions at alternatively splice sites 1,2,3 and 6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, 
            Bischofberger J, Scheiffele P
  TITLE     Targeted proteoform mapping uncovers specific Neurexin-3 variants 
            required for dendritic inhibition.
  JOURNAL   Neuron. 2022 May 8. pii: S0896-6273(22)00362-2. doi: 
            10.1016/j.neuron.2022.04.017.
  PUBMED    35550065
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2022 May 8. pii: S0896-6273(22)00362-2. doi: 
            10.1016/j.neuron.2022.04.017."
COMMENT     Sequence Label: pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+...
FEATURES             Location/Qualifiers
     source          1..10229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(688..690,691..693,694..1404)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(688..690,691..693,694..1404)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(733..754)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(994..1013)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1341..1362
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1429..1482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     sig_peptide     1507..1566
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1570..1596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5980..5997)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5986..6210
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6342..6930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6422..6441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7259..7306
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7331..7350
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7538..8332)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7538..8332)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7649..7668)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8259..8278
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     enhancer        8529..8720
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8677..8697
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8780..9571,9572..9640))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9403..9422
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9641..9745)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9772..10227
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9859..9878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10069..10090
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatctatg gtgagcaagg gcgaggagct gttcaccggg
      721 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      781 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      841 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      901 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      961 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1021 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1081 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1141 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1201 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1261 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1321 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1381 ctcggcatgg acgagctgta caagtccgga ctcagatccg gcagtggaga gggcagagga
     1441 agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagaattcgg cttggggata
     1501 tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc
     1561 actggtgact atccatatga tgttccagat tatgctgggg cccagccggc cagatctttc
     1621 atgggccttc ctaaccagtg ggcccgctac ctgcgttggg atgcaagcac gcgcagtgac
     1681 ctgagcttcc agttcaagac caatgtttcc actgggctgc tcctgtattt ggatgatggt
     1741 ggtgtctgtg acttcctctg tctctccctg gtggatggcc gcgttcagct ccgctttagc
     1801 atggactgtg ccgagaccac tgtgctatcc aacaagcagg tgaacgacag cagttggcac
     1861 ttcctcatgg tgagccgtga tcgtgtgcgc actgggctgg tgattgatgg tgagggccag
     1921 tctggagagc tacggcccca gcggccttat atggatgtgg tcagtgattt gttcctcggt
     1981 ggcgtccccg ctgacattcg accttctgcc ctgaccctcg atggagtaca gagcatgcct
     2041 ggctttaagg gattaatgct ggatctcaag tatggcaact cggaacctcg gcttctgggg
     2101 agccagagcg tccagttaga agcagaggga ccctgtggcg agcgtccctg tgaaaatggt
     2161 gggatctgct tcctcttgga tggtcatccc acctgtgact gttctaccac tggctatggt
     2221 ggcacactct gctcagaaga tgtcagtcaa ggtccaggcc tctcccatct tatgatgagt
     2281 gaacaagcac gagaggagaa cgtggccacc ttccgaggct cagagtatct gtgctatgac
     2341 ctgtcccaga accccatcca gagcagcagc gacgaaatca ccctttcctt taagacctgg
     2401 caacgcaatg ggctcatcct ccacactggc aagtcggccg actatgttaa cctggctctg
     2461 aaggatggtg cggtctcctt ggtcattaac ctggggtccg gggcctttga ggccattgtg
     2521 gagccagtga atgggaaatt caacgacaac gcctggcatg atgtcaaagt gacacgcaat
     2581 cttcggcagg tgacaatctc tgtggatggc atcctaacca caacgggcta cactcaagag
     2641 gactacacca tgctgggctc agatgacttc ttctatgttg gtggaagccc aagtaccgca
     2701 gacttgccag gttcacctgt gagcaacaac ttcatgggct gccttaaaga ggttgtttat
     2761 aagaataatg acatccgtct agagctgtct cgcttggccc ggattggaga caccaagatg
     2821 aaaatctatg gtgaagttgt gttcaagtgt gagaacgtgg ccacactgga tcccatcaac
     2881 tttgagaccc cagaggctta catcagcttg cccaagtgga acaccaaacg tatgggctcc
     2941 atttcctttg actttcgcac cactgagccc aacggcctga ttctcttcac tcacgggaag
     3001 cctcaagaaa ggaaagatgt ccggagccaa aagaacacaa aagttgactt ctttgctgtg
     3061 gaacttcttg atggcaacct gtatttgctg cttgacatgg gctctggcac catcaaagtg
     3121 aaggccactc agaagaaggc caatgatggg gaatggtacc acgtggatat tcagcgtgat
     3181 ggcagatcag gtaccatatc tgtgaacagc aggcgcacgc cattcaccgc cagtggggag
     3241 agtgaaattc tcgacctgga gggggacatg tatctgggcg ggctgccaga gaaccgagct
     3301 ggcctcatcc tccccacgga gctctggacc gccatgctca actacggtta cgtgggctgc
     3361 atccgcgacc tgttcatcga tgggcgcagc aagaacatcc ggcagctggc ggagatgcag
     3421 aacgcagcag gcgtcaagtc ctcctgttca cgcatgagcg ccaagcagtg tgacagctac
     3481 ccctgcaaga acaacgcggt gtgcaaggac ggctggaacc gcttcatctg cgactgcact
     3541 ggcaccggct actgggggag aacctgcgaa agagaggcat ctatcctgag ctatgacggc
     3601 agcatgtaca tgaaggtcat catgcccatg gtgatgcaca cggaggcaga ggatgtgtcc
     3661 ttccgcttca tgtcacagcg agcctatggg ctactggtag ccacaacctc cagggattct
     3721 gcagatacac tgcgtctaga gttggatggt gggcgtgtca agctcatggt taacttagac
     3781 tgtatcagga taaactgtaa ctccagcaaa ggacctgaga ccctgtatgc agggcagaag
     3841 ctcaatgaca atgagtggca caccgtccgg gtagtgcgga gaggaaaaag ccttaagcta
     3901 acagtggatg atgatgtggc tgaggggaca atggtgggcg accacacccg cctggaattc
     3961 cacaatattg aaactggtat catgactgag aagcgttaca tctctgtggt cccctccagt
     4021 ttcattggcc atctacagag cctcatgttc aatggcttac tgtacattga tttgtgcaaa
     4081 aatggcgaca tcgactactg tgaactgaag gctcgctttg gactgagaaa catcatcgcc
     4141 gaccctgtca cttttaagac caagagcagc tacctgaccc tcgccaccct acaggcttac
     4201 acctccatgc acctcttctt ccagttcaag accacttcag ctgatggctt cattctcttc
     4261 aatagcggag atggcaatga ttttattgca gttgagctgg tcaaggggta catacactat
     4321 gtgtttgatc tcggcaatgg tcccaatgtg atcaaaggca acagtgaccg ccccctgaat
     4381 gacaaccagt ggcacaacgt ggtcatcacc agggacagca gtaacaccca cagtctgaag
     4441 gtggacacca aggtagtcac tcaggtcatc aatggtgcca aaaatctgga tttgaaaggt
     4501 gacctctata tggctggctt agcccagggc atgtacagca accttcccaa gcttgtggcc
     4561 tccagggatg gatttcaggg ctgcctggcc tctgtggact tgaatggacg cctgcctgat
     4621 ctcatcaacg atgctctcca caggagtgga cagatcgagc gaggctgtga agttgcgttg
     4681 accaaagctg acctgcaagg acccagtaca acctgtcagg aggattcatg cgccaaccag
     4741 ggggtttgca tgcagcagtg ggaaggtttc acctgtgact gctccatgac atcatattct
     4801 ggaaaccagt gcaatgaccc tggtgcaaca tacatctttg ggaaaagcgg tggcctcatc
     4861 ctctatacct ggccagcaaa tgacagaccc agcacacgct ctgaccgtct cgccgtgggc
     4921 ttcagcacta ctgtgaagga tggtatctta gtacgcattg acagcgcccc tggacttggc
     4981 gacttcctcc agcttcacat agaacaaggg aaaattggag ttgtcttcaa tattggcaca
     5041 gttgacatct ccatcaaaga agagagaact cctgtcaatg atggcaaata ccacgttgtg
     5101 cgcttcacca ggaatggggg aaatgctaca cttcaggtcg acaactggcc agtgaatgag
     5161 cactatccta caggcaacac tgataatgaa cgcctccaaa tggtaaaaca gaaaatcccc
     5221 ttcaaatata accggcccgt agaggagtgg ctgcaggaaa aaggccggca gctaaccatc
     5281 ttcaacaccc aggcgcaaat agccatcgga ggaaaggaca aaggacgtct ctttcaaggt
     5341 caactctctg ggctctatta tgatggtttg aaagtactga acatggcagc tgagaacaac
     5401 cctaatatta aaatcaatgg aagtgtccgg ctagtgggag aagtcccatc agtctcagga
     5461 acaacacaga caacgtccat gccacctgaa atgtctacca ccgtcatgga aaccaccacc
     5521 accatggcta cgaccaccac ccgaaagaac cgctctacag ccagcattca gcccacgtca
     5581 gatgatcttg tttcatctgc tgaatgttca agtgatgatg aagactttgt cgaatgtgaa
     5641 ccaagtacag gtaggtcagc aaaccccacg gagccaggaa tcagacgggt tccgggggcc
     5701 tcagaggtga tccgggagtc cagcagtaca acagggatgg tcgtcggcat tgtggctgct
     5761 gccgccctct gcatcttgat cctcctgtac gccatgtaca agtacaggaa cagggacgag
     5821 gggtcctatc aagtggacga gacgaggaac tacatcagca actcggccca gagcaacggc
     5881 acgctcatga aggagaagca agccagctcc aagagcggcc ataagaaaca gaaaaacaag
     5941 gacaaggagt attatgtgta agcggccgct cgagatcagc ctcgactgtg ccttctagtt
     6001 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6061 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6121 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6181 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agtggcggta
     6241 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7021 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7081 gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct
     7141 tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa
     7201 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga
     7261 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
     7321 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac
     7381 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
     7441 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
     7501 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     7561 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     7621 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     7681 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     7741 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     7801 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     7861 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     7921 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     7981 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     8041 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     8101 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat
     8161 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     8221 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     8281 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     8341 atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct
     8401 cactacttct ggaatagctc agaggccggg cctcggcctc tgcataaata aaaaaaatta
     8461 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     8521 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     8581 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     8641 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     8701 gctggttctt tccgcctcag gactcttcct ttttcaataa atcaatctaa agtatatatg
     8761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
     9781 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     9841 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     9901 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     9961 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    10021 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    10081 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    10141 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    10201 ttaacaaaat attaacgctt acaatttac
//
