LOCUS       sequence-365525-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxA via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxA
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1080
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
ORIGIN
        1 atgaaatttg gaaacttttt gcttacatac caacctcccc aattttctca aacagaggta
       61 atgaaacgtt tggttaaatt aggtcgcatc tctgaggagt gtggttttga taccgtatgg
      121 ttactggagc atcatttcac ggagtttggt ttgcttggta acccttatgt cgctgctgca
      181 tatttacttg gcgcgactaa aaaattgaat gtaggaactg ccgctattgt tcttcccaca
      241 gcccatccag tacgccaact tgaagatgtg aatttattgg atcaaatgtc aaaaggacga
      301 tttcggtttg gtatttgccg agggctttac aacaaggact ttcgcgtatt cggcacagat
      361 atgaataaca gtcgcgcctt agcggaatgc tggtacgggc tgataaagaa tggcatgaca
      421 gagggatata tggaagctga taatgaacat atcaagttcc ataaggtaaa agtaaacccc
      481 gcggcgtata gcagaggtgg cgcaccggtt tatgtggtgg ctgaatcagc ttcgacgact
      541 gagtgggctg ctcaatttgg cctaccgatg atattaagtt ggattataaa tactaacgaa
      601 aagaaagcac aacttgagct ttataatgaa gtggctcaag aatatgggca cgatattcat
      661 aatatcgacc attgcttatc atatataaca tctgtagatc atgactcaat taaagcgaaa
      721 gagatttgcc ggaaatttct ggggcattgg tatgattctt atgtgaatgc tacgactatt
      781 tttgatgatt cagaccaaac aagaggttat gatttcaata aagggcagtg gcgtgacttt
      841 gtattaaaag gacataaaga tactaatcgc cgtattgatt acagttacga aatcaatccc
      901 gtgggaacgc cgcaggaatg tattgacata attcaaaaag acattgatgc tacaggaata
      961 tcaaatattt gttgtggatt tgaagctaat ggaacagtag acgaaattat tgcttccatg
     1021 aagctcttcc agtctgatgt catgccattt cttaaagaaa aacaacgttc gctattatat
//
