LOCUS       sequence-365527-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxB via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxB
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..981
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
ORIGIN
        1 atgaaatttg gattgttctt ccttaacttc atcaattcaa caactgttca agaacaaagt
       61 atagttcgca tgcaggaaat aacggagtat gttgataagt tgaattttga acagatttta
      121 gtgtatgaaa atcatttttc agataatggt gttgtcggcg ctcctctgac tgtttctggt
      181 tttctgctcg gtttaacaga gaaaattaaa attggttcat taaatcacat cattacaact
      241 catcatcctg tccgcatagc ggaggaagct tgcttattgg atcagttaag tgaagggaga
      301 tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg
      361 gttgaatatc aacagcaact atttgaagag tgttatgaaa tcattaacga tgctttaaca
      421 acaggctatt gtaatccaga taacgatttt tatagcttcc ctaaaatatc tgtaaatccc
      481 catgcttata cgccaggcgg acctcggaaa tatgtaacag caaccagtca tcatattgtt
      541 gagtgggcgg ccaaaaaagg tattcctctc atctttaagt gggatgattc taatgatgtt
      601 agatatgaat atgctgaaag atataaagcc gttgcggata aatatgacgt tgacctatca
      661 gagatagacc atcagttaat gatattagtt aactataacg aagatagtaa taaagctaaa
      721 caagagacgc gtgcatttat tagtgattat gttcttgaaa tgcaccctaa tgaaaatttc
      781 gaaaataaac ttgaagaaat aattgcagaa aacgctgtcg gaaattatac ggagtgtata
      841 actgcggcta agttggcaat tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa
      901 ccaatgaatg atttgatgag ccaaaaaaat gtaatcaata ttgttgatga taatattaag
      961 aagtaccaca tggaatatac c
//
