LOCUS       sequence-365531-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxD via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxD
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..921
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
ORIGIN
        1 atggaaaatg aatcaaaata taaaaccatc gaccacgtta tttgtgttga aggaaataaa
       61 aaaattcatg tttgggaaac gctgccagaa gaaaacagcc caaagagaaa gaatgccatt
      121 attattgcgt ctggttttgc ccgcaggatg gatcattttg ctggtctggc ggaatattta
      181 tcgcggaatg gatttcatgt gatccgctat gattcgcttc accacgttgg attgagttca
      241 gggacaattg atgaatttac aatgtctata ggaaagcaga gcttgttagc agtggttgat
      301 tggttaacta cacgaaaaat aaataacttc ggtatgttgg cttcaagctt atctgcgcgg
      361 atagcttatg caagcctatc tgaaatcaat gcttcgtttt taatcaccgc agtcggtgtt
      421 gttaacttaa gatattctct tgaaagagct ttagggtttg attatctcag tctacccatt
      481 aatgaattgc cgaataatct agattttgaa ggccataaat tgggtgctga agtctttgcg
      541 agagattgtc ttgattttgg ttgggaagat ttagcttcta caattaataa catgatgtat
      601 cttgatatac cgtttattgc ttttactgca aataacgata attgggtcaa gcaagatgaa
      661 gttatcacat tgttatcaaa tattcgtagt aatcgatgca agatatattc tttgttagga
      721 agttcgcatg acttgagtga aaatttagtg gtcctgcgca atttttatca atcggttacg
      781 aaagccgcta tcgcgatgga taatgatcat ctggatattg atgttgatat tactgaaccg
      841 tcatttgaac atttaactat tgcgacagtc aatgaacgcc gaatgagaat tgagattgaa
      901 aatcaagcaa tttctctgtc t
//
