LOCUS       sequence-365533-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxE via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxE
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1110
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
ORIGIN
        1 atgacttcat atgttgataa acaagaaatt acagcaagct cagaaattga tgatttgatt
       61 ttttcgagcg atccattagt gtggtcttac gacgagcagg aaaaaatcag aaagaaactt
      121 gtgcttgatg catttcgtaa tcattataaa cattgtcgag aatatcgtca ctactgtcag
      181 gcacacaaag tagatgacaa tattacggaa attgatgaca tacctgtatt cccaacatcg
      241 gtttttaagt ttactcgctt attaacttct caggaaaacg agattgaaag ttggtttacc
      301 agtagcggca cgaatggttt aaaaagtcag gtggcgcgtg acagattaag tattgagaga
      361 ctcttaggct ctgtgagtta tggcatgaaa tatgttggta gttggtttga tcatcaaata
      421 gaattagtca atttgggacc agatagattt aatgctcata atatttggtt taaatatgtt
      481 atgagtttgg tggaattgtt atatcctacg acatttaccg taacagaaga acgaatagat
      541 tttgttaaaa cattgaatag tcttgaacga ataaaaaatc aagggaaaga tctttgtctt
      601 attggttcgc catactttat ttatttactc tgccattata tgaaagataa aaaaatctca
      661 ttttctggag ataaaagcct ttatatcata accggaggcg gctggaaaag ttacgaaaaa
      721 gaatctctga aacgtgatga tttcaatcat cttttatttg atactttcaa tctcagtgat
      781 attagtcaga tccgagatat atttaatcaa gttgaactca acacttgttt ctttgaggat
      841 gaaatgcagc gtaaacatgt tccgccgtgg gtatatgcgc gagcgcttga tcctgaaacg
      901 ttgaaacctg tacctgatgg aacgccgggg ttgatgagtt atatggatgc gtcagcaacc
      961 agttatccag catttattgt taccgatgat gtcgggataa ttagcagaga atatggtaag
     1021 tatcccggcg tgctcgttga aattttacgt cgcgtcaata cgaggacgca gaaagggtgt
     1081 gctttaagct taaccgaagc gtttgatagt
//
