LOCUS       sequence-365535-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Fre via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-Fre
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgacgaccc tgtcctgtaa agttacgagc gtagaagcga tcacggatac cgtctaccgc
       61 gtgagaatag tccctgatgc tgccttcagt tttagggcgg ggcagtacct aatggttgtg
      121 atggatgaac gcgacaaacg accgtttagc atggcgagta ccccggacga gaagggcttt
      181 attgaactgc atattggcgc atcagaaatc aatttatatg caaaagctgt gatggaccgc
      241 attctgaaag atcaccagat tgtcgttgat atcccgcatg gcgaagcatg gctgcgggat
      301 gatgaagagc gtccaatgat tcttatcgct ggtggcactg gtttcagcta cgcccggtcg
      361 atattgctca cagcgctggc ccgtaacccg aaccgtgata ttaccatcta ttgggggggt
      421 cgtgaagaac aacacttgta tgatttgtgc gagctggagg ccctgtcgct gaagcatccc
      481 ggcctgcagg tagtgccggt agtggaacaa ccagaggctg ggtggcgtgg acgcactggc
      541 acagttctta ccgcagtgct ccaggaccat ggaacgttag ccgaacacga catttatatt
      601 gccggtcgct ttgagatggc aaaaatcgcg cgcgatttat tttgctctga acgtaatgcg
      661 cgcgaagacc gtctgttcgg tgatgcgttc gcgtttatc
//
